Basic Information | |
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Species | Gossypium raimondii |
Cazyme ID | Gorai.008G236700.1 |
Family | GH32 |
Protein Properties | Length: 650 Molecular Weight: 72423.6 Isoelectric Point: 6.1332 |
Chromosome | Chromosome/Scaffold: 08 Start: 52253391 End: 52257448 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 121 | 439 | 0 |
HFQPEKNWMNDPNGPLFYKGWYHFFYQYNPHAAVWGDIVWGHAVSKDLIHWLHLPLAMVADQWYDKNGVWTGSATILPDGKVVMLYTGSTTENVQVQNLA YPADHSDPLLINWVKYSGNPVLVPPPGILDKDFRDPTTAWLTSEGKWRITIGSKINKTGIALVYDTKDFIYYELLDGVLHAVPGTGMWECVDFFPVSKTE ENGLDTSVNGPSVKHVVKASLDDDRHDYYSIGTYHEKNGTWIPDEPKIDVGIGLRYDYGIYYAAKTFYDQNKNRRVLWGWIGESDSEAADMQKGWASVQS IPRTILFDKKTGTHLLQWP |
Full Sequence |
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Protein Sequence Length: 650 Download |
MVDTRPFLPV SSHAGSDPTG PSENRRKPMK LQLSIFFVLL TVGFFAALLT SNNGSDNGFV 60 KENEHGSLAS PSSEKQLRPV SRGPAVGVSE KSNRLFAKDN ENQLAYPWNN SMLSWQRTAF 120 HFQPEKNWMN DPNGPLFYKG WYHFFYQYNP HAAVWGDIVW GHAVSKDLIH WLHLPLAMVA 180 DQWYDKNGVW TGSATILPDG KVVMLYTGST TENVQVQNLA YPADHSDPLL INWVKYSGNP 240 VLVPPPGILD KDFRDPTTAW LTSEGKWRIT IGSKINKTGI ALVYDTKDFI YYELLDGVLH 300 AVPGTGMWEC VDFFPVSKTE ENGLDTSVNG PSVKHVVKAS LDDDRHDYYS IGTYHEKNGT 360 WIPDEPKIDV GIGLRYDYGI YYAAKTFYDQ NKNRRVLWGW IGESDSEAAD MQKGWASVQS 420 IPRTILFDKK TGTHLLQWPV EEIESLRLKS YEFNQVKVQA GSVVPLDVGP ATQLDIIAEF 480 EIDKEALEKT TGSNVTFSCS SSGGTIERGA LGPFGLLVLA DDSLSEQTPV YFYIAKGSDG 540 KLKTFFCNDQ SRSSVASDVN KQIYGNLVPV LEGEKLSLRV LVDHSIVESF AQGGRTVITS 600 RVYPTKAIYG AAKLFLFNNA TEASVSASLR IWQMNSAFIR PYPNNKESQ* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-58 | 117 | 604 | 508 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-77 | 117 | 605 | 498 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 3.0e-100 | 127 | 442 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-155 | 121 | 439 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 121 | 594 | 484 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAB82419.1 | 0 | 62 | 649 | 66 | 651 | acid invertase [Citrus unshiu] |
DDBJ | BAF34362.1 | 0 | 69 | 649 | 74 | 651 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35859.1 | 0 | 1 | 642 | 1 | 649 | soluble acid invertase [Pyrus pyrifolia var. culta] |
RefSeq | XP_002303519.1 | 0 | 1 | 647 | 1 | 662 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002532576.1 | 0 | 1 | 644 | 1 | 647 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 101 | 642 | 3 | 544 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
PDB | 3ugh_A | 0 | 101 | 642 | 3 | 544 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
PDB | 3ugg_B | 0 | 101 | 642 | 3 | 544 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
PDB | 3ugg_A | 0 | 101 | 642 | 3 | 544 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
PDB | 3ugf_B | 0 | 101 | 642 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |