| Basic Information | |
|---|---|
| Species | Gossypium raimondii |
| Cazyme ID | Gorai.008G236700.3 |
| Family | GH32 |
| Protein Properties | Length: 647 Molecular Weight: 72097.3 Isoelectric Point: 6.2472 |
| Chromosome | Chromosome/Scaffold: 08 Start: 52253438 End: 52257349 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 121 | 436 | 0 |
| HFQPEKNWMNGPLFYKGWYHFFYQYNPHAAVWGDIVWGHAVSKDLIHWLHLPLAMVADQWYDKNGVWTGSATILPDGKVVMLYTGSTTENVQVQNLAYPA DHSDPLLINWVKYSGNPVLVPPPGILDKDFRDPTTAWLTSEGKWRITIGSKINKTGIALVYDTKDFIYYELLDGVLHAVPGTGMWECVDFFPVSKTEENG LDTSVNGPSVKHVVKASLDDDRHDYYSIGTYHEKNGTWIPDEPKIDVGIGLRYDYGIYYAAKTFYDQNKNRRVLWGWIGESDSEAADMQKGWASVQSIPR TILFDKKTGTHLLQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 647 Download |
| MVDTRPFLPV SSHAGSDPTG PSENRRKPMK LQLSIFFVLL TVGFFAALLT SNNGSDNGFV 60 KENEHGSLAS PSSEKQLRPV SRGPAVGVSE KSNRLFAKDN ENQLAYPWNN SMLSWQRTAF 120 HFQPEKNWMN GPLFYKGWYH FFYQYNPHAA VWGDIVWGHA VSKDLIHWLH LPLAMVADQW 180 YDKNGVWTGS ATILPDGKVV MLYTGSTTEN VQVQNLAYPA DHSDPLLINW VKYSGNPVLV 240 PPPGILDKDF RDPTTAWLTS EGKWRITIGS KINKTGIALV YDTKDFIYYE LLDGVLHAVP 300 GTGMWECVDF FPVSKTEENG LDTSVNGPSV KHVVKASLDD DRHDYYSIGT YHEKNGTWIP 360 DEPKIDVGIG LRYDYGIYYA AKTFYDQNKN RRVLWGWIGE SDSEAADMQK GWASVQSIPR 420 TILFDKKTGT HLLQWPVEEI ESLRLKSYEF NQVKVQAGSV VPLDVGPATQ LDIIAEFEID 480 KEALEKTTGS NVTFSCSSSG GTIERGALGP FGLLVLADDS LSEQTPVYFY IAKGSDGKLK 540 TFFCNDQSRS SVASDVNKQI YGNLVPVLEG EKLSLRVLVD HSIVESFAQG GRTVITSRVY 600 PTKAIYGAAK LFLFNNATEA SVSASLRIWQ MNSAFIRPYP NNKESQ* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 6.0e-54 | 117 | 601 | 508 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 4.0e-72 | 117 | 602 | 498 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 1.0e-94 | 127 | 439 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 5.0e-150 | 121 | 436 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 121 | 591 | 484 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB82419.1 | 0 | 62 | 646 | 66 | 651 | acid invertase [Citrus unshiu] |
| DDBJ | BAF34362.1 | 0 | 69 | 646 | 74 | 651 | beta-fructofuranosidase [Citrus sinensis] |
| DDBJ | BAF35859.1 | 0 | 1 | 639 | 1 | 649 | soluble acid invertase [Pyrus pyrifolia var. culta] |
| RefSeq | XP_002303519.1 | 0 | 1 | 644 | 1 | 662 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532576.1 | 0 | 1 | 641 | 1 | 647 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 101 | 639 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| PDB | 3ugh_A | 0 | 101 | 639 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| PDB | 3ugg_B | 0 | 101 | 639 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| PDB | 3ugg_A | 0 | 101 | 639 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| PDB | 3ugf_B | 0 | 101 | 639 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
