Basic Information | |
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Species | Gossypium raimondii |
Cazyme ID | Gorai.010G099300.3 |
Family | GH32 |
Protein Properties | Length: 403 Molecular Weight: 45670.3 Isoelectric Point: 6.1851 |
Chromosome | Chromosome/Scaffold: 10 Start: 17521780 End: 17524608 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 52 | 367 | 0 |
HFQPPKNWMNDPNGPMYYKGVYHLFYQYNPYAAVWGAITWAHSISYDLVNWIHLDIALSPDDPFDINGCWSGSTTFLSGGKPVILYTGGVTMNRQVQNLA EPKNLSDPMLREWVKSSHNPIITHVDGMDSENFRDPTTAWQGPDGLWRVLVGNEMNSHGRALLYRSRDFVTWTQSKEPIHSSTRTGMWECPDFYPVSLDG KNGVDTSSLDKFTKHVLKASFDSSDHYVLGNYTAVTDNFSVDTDFLENGSDLRYDYGNFYASKTFFDSAKKRRILWAWIQESDSTTDDIKKDWSGLQSFP RSILLSKTGKQLIQWP |
Full Sequence |
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Protein Sequence Length: 403 Download |
MTDLKLKVTM DLSVVLVVGF CFVLLGNGVQ SDRDDKKLQS LPQDQPYRTG YHFQPPKNWM 60 NDPNGPMYYK GVYHLFYQYN PYAAVWGAIT WAHSISYDLV NWIHLDIALS PDDPFDINGC 120 WSGSTTFLSG GKPVILYTGG VTMNRQVQNL AEPKNLSDPM LREWVKSSHN PIITHVDGMD 180 SENFRDPTTA WQGPDGLWRV LVGNEMNSHG RALLYRSRDF VTWTQSKEPI HSSTRTGMWE 240 CPDFYPVSLD GKNGVDTSSL DKFTKHVLKA SFDSSDHYVL GNYTAVTDNF SVDTDFLENG 300 SDLRYDYGNF YASKTFFDSA KKRRILWAWI QESDSTTDDI KKDWSGLQSF PRSILLSKTG 360 KQLIQWPVEE IEKQRTVNVS FKNKELKGGS VLEISGITAS QV* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 1.0e-57 | 36 | 376 | 361 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 3.0e-72 | 43 | 394 | 361 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 9.0e-95 | 58 | 370 | 321 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 5.0e-144 | 52 | 367 | 326 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 3.0e-144 | 52 | 401 | 359 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAD19323.1 | 0 | 9 | 402 | 3 | 398 | exocellular acid invertase 2 [Beta vulgaris] |
RefSeq | XP_002278898.1 | 0 | 60 | 402 | 1 | 341 | PREDICTED: hypothetical protein isoform 2 [Vitis vinifera] |
RefSeq | XP_002278918.1 | 0 | 10 | 402 | 1 | 391 | PREDICTED: hypothetical protein isoform 3 [Vitis vinifera] |
RefSeq | XP_002279133.1 | 0 | 64 | 402 | 2 | 337 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002309496.1 | 0 | 10 | 402 | 1 | 392 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 44 | 402 | 4 | 365 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2ade_A | 0 | 44 | 402 | 4 | 365 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 2add_A | 0 | 44 | 402 | 4 | 365 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
PDB | 1st8_A | 0 | 44 | 402 | 4 | 365 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 44 | 402 | 4 | 365 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |