Basic Information | |
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Species | Gossypium raimondii |
Cazyme ID | Gorai.010G241200.2 |
Family | GT20 |
Protein Properties | Length: 711 Molecular Weight: 81336.9 Isoelectric Point: 7.4539 |
Chromosome | Chromosome/Scaffold: 10 Start: 61065683 End: 61068850 |
Description | trehalose-phosphatase/synthase 7 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 57 | 546 | 0 |
SVNQDRIIIVANQLPVKAKRGPDNKGWSFNWDDYSLLLQLKDGLPEEMEVLYVGSLKIDVDINEQDEVSSVLLEKFKCVPAFLPPEIFNKFYHGFCKQHL WSLFHYMLPYSANHGGRFDRSLWEAYVAANKIFSQRVIEVINPEDDYIWIHDYHLMVLPTFLRRRFNRLRMGFFLHSPFPSSEIYRTSPVREEILKALLN SDLIGFHTFDYARHFLSCCSRMLGLEYQSKRGYIGLEYYGRTIGIKIMPVGVHMEQIESALRVADKESRIHEFKQKFDGKTVLLGVDDMDVFKGVNLKLL AMEQMLKQHPKWQGRAVLVQITNPARGRGKDLEDTQAEIQASCKRINEMFGKPGYEPIVFIDRPISLTERVAYYTIAECVVVTAVRDGMNLTPYEYIACR QGVSESASSSESNGPKKSKLVVSEFIGCSPSLSGAIRVNPWNFEATAEAMNEAISMADAETQLQHEKHYRYVSSHDVAFWSHSFFQDMER |
Full Sequence |
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Protein Sequence Length: 711 Download |
MMSRSYTNLL DLASGNFPVM GPVRERKRLT RVMTVPGVVS ELDDDQANSV NSDVHSSVNQ 60 DRIIIVANQL PVKAKRGPDN KGWSFNWDDY SLLLQLKDGL PEEMEVLYVG SLKIDVDINE 120 QDEVSSVLLE KFKCVPAFLP PEIFNKFYHG FCKQHLWSLF HYMLPYSANH GGRFDRSLWE 180 AYVAANKIFS QRVIEVINPE DDYIWIHDYH LMVLPTFLRR RFNRLRMGFF LHSPFPSSEI 240 YRTSPVREEI LKALLNSDLI GFHTFDYARH FLSCCSRMLG LEYQSKRGYI GLEYYGRTIG 300 IKIMPVGVHM EQIESALRVA DKESRIHEFK QKFDGKTVLL GVDDMDVFKG VNLKLLAMEQ 360 MLKQHPKWQG RAVLVQITNP ARGRGKDLED TQAEIQASCK RINEMFGKPG YEPIVFIDRP 420 ISLTERVAYY TIAECVVVTA VRDGMNLTPY EYIACRQGVS ESASSSESNG PKKSKLVVSE 480 FIGCSPSLSG AIRVNPWNFE ATAEAMNEAI SMADAETQLQ HEKHYRYVSS HDVAFWSHSF 540 FQDMERTCKD HFRRRCWGIG LSFGFRVVAL DPNFRKLSID HIESVYVRSK NRAILLDYDG 600 TVMPQTSHNK TPNAEVISIV NRLLGDTKNT VFVVSGRGKE SLDKWFSPCK KLGIAAEHGY 660 FMRWSSDDKW EVCGPNSEFR WKQIAEPVMR LYTEATDGSS IETKENVRPS * 720 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR02400 | trehalose_OtsA | 4.0e-136 | 62 | 546 | 490 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
PRK14501 | PRK14501 | 4.0e-153 | 62 | 705 | 656 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
PLN02205 | PLN02205 | 0 | 1 | 705 | 712 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
pfam00982 | Glyco_transf_20 | 0 | 62 | 546 | 489 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
cd03788 | GT1_TPS | 0 | 62 | 545 | 484 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACD56625.1 | 0 | 11 | 707 | 154 | 850 | trehalose synthase/phosphatase-like protein [Gossypium raimondii] |
GenBank | ACD56638.1 | 0 | 1 | 707 | 1 | 707 | trehalose synthase-like protein [Gossypioides kirkii] |
RefSeq | XP_002305726.1 | 0 | 1 | 706 | 1 | 702 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002317392.1 | 0 | 1 | 707 | 1 | 707 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002522255.1 | 0 | 1 | 706 | 1 | 706 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2wtx_D | 0 | 144 | 546 | 73 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_C | 0 | 144 | 546 | 73 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_B | 0 | 144 | 546 | 73 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_A | 0 | 144 | 546 | 73 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 1gz5_D | 0 | 144 | 546 | 72 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO777775 | 630 | 83 | 706 | 0 |
HO779327 | 512 | 103 | 612 | 0 |
DT563898 | 308 | 332 | 639 | 0 |
HO779327 | 100 | 608 | 707 | 2e-31 |
HO777775 | 80 | 1 | 79 | 0.00002 |
Sequence Alignments (This image is cropped. Click for full image.) |
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