Basic Information | |
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Species | Gossypium raimondii |
Cazyme ID | Gorai.011G147600.3 |
Family | CBM20 |
Protein Properties | Length: 1128 Molecular Weight: 123770 Isoelectric Point: 5.4392 |
Chromosome | Chromosome/Scaffold: 11 Start: 24012041 End: 24023751 |
Description | catalytics;carbohydrate kinases;phosphoglucan, water dikinases |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
CBM20 | 26 | 105 | 2.3e-21 |
NICLDHQVQFGEHVVILGSTKELGSWKKQVPMNWSEDGWICDLELKGGESVEFKFVVVSKDKSVAWEGGNNRVLKLPQGG |
Full Sequence |
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Protein Sequence Length: 1128 Download |
MEREEEKKKK RTKVKPKSGS GKVGLNICLD HQVQFGEHVV ILGSTKELGS WKKQVPMNWS 60 EDGWICDLEL KGGESVEFKF VVVSKDKSVA WEGGNNRVLK LPQGGSFGMI CHWNSTEETL 120 ELLPLSSEEY DDSVDDAGHS ESTSTTDALE VEASPFVGQW QGRPASFMRS NEHHNRELER 180 RWDTTGLEGL ALKLVEGDKS ARNWWRKLEV VRELLVGSLQ SEERLEALIC SAIYLKWINT 240 GQIPCFEDGG HHRPNRHAEI SRLIFRELER ISSRKDSSPQ ELLVIRKIHP CLPSFKAEFT 300 ASVPLTRIRD IAHRNDIPHD LKQEIKHTIQ NKLHRNAGPE DLVATEAMLA RITRDPGQYS 360 EAFVEQFKIF HLELKDFFNA GSLTEQLESI RESLDERGIA ALVMFLECKK SLDAAEGSSS 420 ILDLIKTMRS LGALREVIVR GLESGLRNDA PDAAIAMRQK WRLCEIGLED YSFVLLSRLL 480 NMLEAVGGAN WFADNLESKN ISSWNDPLGA LIVGVHQLSL SGWKPEECAA IQNELTAWQE 540 KGLFAKEGSE DGKRIWALRL KATLDRSRRL TEEYSEVLLQ LFPQKVQMLG KALGIPENSI 600 RTYAEAEIRA GVIFQVSKLC SLLLKAVRTA LGSEGWDVLV PGVVSGTLVQ VENIVPGSLP 660 SSLEGPVILV VNKADGDEEV TAAGSNIAGV VLLQELPHLS HLGVRARQEK VIFVTCEDEE 720 KVSYIQKLEG KCVRLEASSS GVSISPSSLD DRDADSVAKN LSTNGSSAVY MRGPPDLTGL 780 SPKASYSNKG SSSAGLILLA DADAQTSGAK AAACGRLASL AAVSDKVYSD LGVPASFRVP 840 AGVVIPFGSM EWALEQNKSM ETFMSLREKI ETARLEDGEL DNLCHQLQQL VSSVQPPQDL 900 IDSIMRVFPG NVRLIVRSSA NVEDLAGMSA AGLYESIPNV SPSNPTVFAS AVSQVWASLY 960 TRRAVLSRRA AGVSQKDATM AVLVQEMLAP DLSFVLHTLS PTDHDHNYVE AEIAPGLGET 1020 LASGTRGTPW RLSSGKFDGL VKTVAFANFS EEMVVSGASP ADGEVIRLTV DYSKKPLTVD 1080 PVFRQQLSQR LSAVGFFLER KFGCPQDVEG CVLGKDIYVV QTRPQPL* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
COG0574 | PpsA | 2.0e-16 | 835 | 1124 | 306 | + Phosphoenolpyruvate synthase/pyruvate phosphate dikinase [Carbohydrate transport and metabolism] | ||
TIGR01418 | PEP_synth | 2.0e-21 | 833 | 1125 | 312 | + phosphoenolpyruvate synthase. Also called pyruvate,water dikinase and PEP synthase. The member from Methanococcus jannaschii contains a large intein. This enzyme generates phosphoenolpyruvate (PEP) from pyruvate, hydrolyzing ATP to AMP and releasing inorganic phosphate in the process. The enzyme shows extensive homology to other enzymes that use PEP as substrate or product. This enzyme may provide PEP for gluconeogenesis, for PTS-type carbohydrate transport systems, or for other processes [Energy metabolism, Glycolysis/gluconeogenesis]. | ||
PRK06241 | PRK06241 | 5.0e-34 | 835 | 1124 | 299 | + phosphoenolpyruvate synthase; Validated | ||
cd05818 | CBM20_water_dikinase | 1.0e-39 | 25 | 113 | 89 | + Phosphoglucan water dikinase (also known as alpha-glucan water dikinase), N-terminal CBM20 (carbohydrate-binding module, family 20) domain. This domain is found in the chloroplast-encoded phosphoglucan water dikinase, one of two enzymes involved in the phosphorylation of plant starches. In addition to the CBM20 domain, phosphoglucan water dikinase contains a C-terminal pyruvate binding domain. The CBM20 domain is found in a large number of starch degrading enzymes including alpha-amylase, beta-amylase, glucoamylase, and CGTase (cyclodextrin glucanotransferase). CBM20 is also present in proteins that have a regulatory role in starch metabolism in plants (e.g. alpha-amylase) or glycogen metabolism in mammals (e.g. laforin). CBM20 folds as an antiparallel beta-barrel structure with two starch binding sites. These two sites are thought to differ functionally with site 1 acting as the initial starch recognition site and site 2 involved in the specific recognition of appropriate regions of starch. | ||
pfam01326 | PPDK_N | 6.0e-52 | 835 | 1125 | 294 | + Pyruvate phosphate dikinase, PEP/pyruvate binding domain. This enzyme catalyzes the reversible conversion of ATP to AMP, pyrophosphate and phosphoenolpyruvate (PEP). |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005524 | ATP binding |
GO:0005975 | carbohydrate metabolic process |
GO:0016301 | kinase activity |
GO:0016310 | phosphorylation |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAU93516.1 | 0 | 5 | 1127 | 61 | 1196 | chloroplast alpha-glucan water dikinase isoform 3 [Arabidopsis thaliana] |
EMBL | CBI39424.1 | 0 | 1 | 1126 | 60 | 1148 | unnamed protein product [Vitis vinifera] |
RefSeq | NP_198009.3 | 0 | 5 | 1127 | 61 | 1196 | ATGWD3; carbohydrate kinase/ catalytic/ phosphoglucan, water dikinase [Arabidopsis thaliana] |
RefSeq | XP_002265211.1 | 0 | 1 | 1126 | 60 | 1187 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002518612.1 | 0 | 19 | 1126 | 72 | 1174 | chloroplast alpha-glucan water dikinase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1kum_A | 0.0000003 | 23 | 104 | 7 | 95 | A Chain A, The Crystal Structure Of The Phosphoenolpyruvate Synthase From Neisseria Meningitidis |
PDB | 1kul_A | 0.0000003 | 23 | 104 | 7 | 95 | A Chain A, The Crystal Structure Of The Phosphoenolpyruvate Synthase From Neisseria Meningitidis |
PDB | 1acz_A | 0.0000003 | 23 | 104 | 7 | 95 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, 5 Structures |
PDB | 1ac0_A | 0.0000003 | 23 | 104 | 7 | 95 | A Chain A, Glucoamylase, Granular Starch-Binding Domain Complex With Cyclodextrin, Nmr, Minimized Average Structure |
PDB | 2ols_A | 0.0000004 | 803 | 1127 | 16 | 338 | A Chain A, The Crystal Structure Of The Phosphoenolpyruvate Synthase From Neisseria Meningitidis |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO778000 | 888 | 259 | 1128 | 0 |
HO794745 | 735 | 404 | 1128 | 0 |
CO076712 | 297 | 796 | 1092 | 0 |
ES796736 | 318 | 720 | 1037 | 0 |
HO794745 | 45 | 366 | 410 | 0.0000000000004 |
Sequence Alignments (This image is cropped. Click for full image.) |
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