Basic Information | |
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Species | Gossypium raimondii |
Cazyme ID | Gorai.013G220200.3 |
Family | GH32 |
Protein Properties | Length: 588 Molecular Weight: 66633.7 Isoelectric Point: 4.7489 |
Chromosome | Chromosome/Scaffold: 13 Start: 54010261 End: 54013247 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 52 | 368 | 0 |
HFQSPQNWLNDPNGPMYYKGVYHLFYQYNPNSAWFGDGMVWAHSASYDLINWFGLDHALVPSEPFDAISCWSGSATILPGDKPVILYTGIDANYNQVQNL ANPKNESDPLLVEWVKYSRNPLMTPPDGVKGDNFRDPTTAWQGPDGTWKVVIGSYSNSQGMAILYQSQDFVHWTMHRDPLYSSSKTEMWECPDFFPVSIN STNGVDTSVENPSVRHVMKASFNSHDYYIVGTYVTEQERFLPDADFTGTSSDLRFDYGKFYASKSFFDGKKNRRILCAWVNESDSMEDDLKKGWSGLQSI PRQIWLDLNGKQLVQWP |
Full Sequence |
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Protein Sequence Length: 588 Download |
MDISLFLFVG FSCFLLINGN KVEDCDYLTD KTSLQSFHVL LQAEQPYRTA YHFQSPQNWL 60 NDPNGPMYYK GVYHLFYQYN PNSAWFGDGM VWAHSASYDL INWFGLDHAL VPSEPFDAIS 120 CWSGSATILP GDKPVILYTG IDANYNQVQN LANPKNESDP LLVEWVKYSR NPLMTPPDGV 180 KGDNFRDPTT AWQGPDGTWK VVIGSYSNSQ GMAILYQSQD FVHWTMHRDP LYSSSKTEMW 240 ECPDFFPVSI NSTNGVDTSV ENPSVRHVMK ASFNSHDYYI VGTYVTEQER FLPDADFTGT 300 SSDLRFDYGK FYASKSFFDG KKNRRILCAW VNESDSMEDD LKKGWSGLQS IPRQIWLDLN 360 GKQLVQWPVE ELNSLRDNEV YVYDKQLESG SVFEVSGITA SQADIEIMFE LPKLEEAEFI 420 DTSLVDPQLI CDKQDASVTG KFGPFGLLAL ATKDLTEQTA IFFRVFRDHK DYKVLMCSDQ 480 KRSSLRNELD KTTYGAFVDI DPQREMVSLR TLIDHSIIES FGGKGRSVIT TRVYPKLAIN 540 DEAHLFAFNN GSLSVRVSRL NAWSMNKAQI TAGGEPGTIS EDFSRKK* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 5.0e-55 | 42 | 536 | 518 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 9.0e-69 | 43 | 535 | 508 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 3.0e-87 | 58 | 371 | 320 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-133 | 52 | 368 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 4.0e-165 | 52 | 525 | 484 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAD49079.1 | 0 | 36 | 570 | 34 | 568 | fructan 1-exohydrolase [Campanula rapunculoides] |
RefSeq | XP_002278898.1 | 0 | 60 | 571 | 1 | 510 | PREDICTED: hypothetical protein isoform 2 [Vitis vinifera] |
RefSeq | XP_002278918.1 | 0 | 1 | 571 | 1 | 560 | PREDICTED: hypothetical protein isoform 3 [Vitis vinifera] |
RefSeq | XP_002309496.1 | 0 | 1 | 572 | 1 | 561 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002309497.1 | 0 | 1 | 570 | 1 | 566 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 42 | 571 | 2 | 534 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 2ade_A | 0 | 42 | 571 | 2 | 534 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 2add_A | 0 | 42 | 571 | 2 | 534 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 1st8_A | 0 | 42 | 571 | 2 | 534 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 42 | 571 | 2 | 534 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |