| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os01g09260.1 |
| Family | AA7 |
| Protein Properties | Length: 533 Molecular Weight: 56034.4 Isoelectric Point: 6.2435 |
| Chromosome | Chromosome/Scaffold: 1 Start: 4697314 End: 4699792 |
| Description | cytokinin oxidase 4 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 67 | 348 | 4.7e-24 |
| ALPAAVLFPGSPGDVAELLRAAYAAPGRPFTVSFRGRGHSTMGQALAAGGVVVHMQSMGGGGAPRINVSADGAYVDAGGEQLWVDVLRAALARGVAPRSW TDYLHLTVGGTLSNAGVSGQTYRHGPQISNVLELDVITGHGETVTCSKAVNSDLFDAVLGGLGQFGVITRARVAVEPAPARARWVRLVYADFAAFSADQE RLVAARPDGSHGPWSYVEGAVYLAGRGLAVALKSSGGFFSDADAARVVALAAARNATAVYSIEATLNYAANATPSSVDAAVA | |||
| Full Sequence |
|---|
| Protein Sequence Length: 533 Download |
| MAAIYLLIAA LIASSHALAA HGAGGGVPLA AAAPLPFPGD LAASGKLRTD PNATVPASMD 60 FGNITAALPA AVLFPGSPGD VAELLRAAYA APGRPFTVSF RGRGHSTMGQ ALAAGGVVVH 120 MQSMGGGGAP RINVSADGAY VDAGGEQLWV DVLRAALARG VAPRSWTDYL HLTVGGTLSN 180 AGVSGQTYRH GPQISNVLEL DVITGHGETV TCSKAVNSDL FDAVLGGLGQ FGVITRARVA 240 VEPAPARARW VRLVYADFAA FSADQERLVA ARPDGSHGPW SYVEGAVYLA GRGLAVALKS 300 SGGFFSDADA ARVVALAAAR NATAVYSIEA TLNYAANATP SSVDAAVAAA LGDLHFEEGF 360 SFSRDVTYEE FLDRVYGEEE ALEKAGLWRV PHPWLNLFVP GSRIADFDRG VFKGILQTAT 420 DIAGPLIIYP VNKSKWDAAM SAVTPEGEEE VFYVVSLLFS AVANDVAALE AQNRRILRFC 480 DLAGIGYKAY LAHYDSRGDW VRHFGAKWDR FVQRKDKYDP KKLLSPGQDI FN* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01678 | FAD_lactone_ox | 4.0e-5 | 60 | 234 | 179 | + sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1. | ||
| COG0277 | GlcD | 6.0e-20 | 44 | 528 | 494 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 4.0e-22 | 69 | 213 | 146 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 5.0e-110 | 253 | 531 | 283 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 45 | 532 | 492 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005623 | cell |
| GO:0008152 | metabolic process |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009056 | catabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1W1O | 0 | 5 | 532 | 6 | 534 | A Chain A, Native Cytokinin Dehydrogenase |
| GenBank | ADB45878.1 | 0 | 1 | 532 | 1 | 525 | cytokinin oxidase/dehydrogenase [Bambusa oldhamii] |
| GenBank | ADB45880.1 | 0 | 1 | 532 | 1 | 522 | cytokinin oxidase/dehydrogenase [Bambusa oldhamii] |
| GenBank | EAY72836.1 | 0 | 1 | 532 | 1 | 532 | hypothetical protein OsI_00707 [Oryza sativa Indica Group] |
| RefSeq | NP_001105591.1 | 0 | 5 | 532 | 6 | 534 | cytokinin oxidase1 precursor [Zea mays] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1w1s_A | 0 | 5 | 532 | 6 | 534 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1w1r_A | 0 | 5 | 532 | 6 | 534 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1w1q_A | 0 | 5 | 532 | 6 | 534 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1w1o_A | 0 | 5 | 532 | 6 | 534 | A Chain A, Native Cytokinin Dehydrogenase |
| PDB | 3s1c_A | 0 | 36 | 532 | 15 | 516 | A Chain A, Native Cytokinin Dehydrogenase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cytokinins degradation | RXN-4621 | EC-1.5.99 | isopentenyladenine:FAD oxidoreductase |
| cytokinins degradation | RXN-4641 | EC-1.5.99 | isopentenyl adenosine:FAD oxidoreductase |
| cytokinins degradation | RXN-4661 | EC-1.5.99 | trans-zeatin:FAD oxidoreductase |
| cytokinins degradation | RXN-4662 | EC-1.5.99 | cis-zeatin:FAD oxidoreductase |
| cytokinins degradation | RXN-4681 | EC-1.5.99 | trans-zeatin riboside:FAD oxidoreductase |
