| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os01g16310.1 |
| Family | GH31 |
| Protein Properties | Length: 933 Molecular Weight: 103388 Isoelectric Point: 9.5354 |
| Chromosome | Chromosome/Scaffold: 1 Start: 9245577 End: 9253454 |
| Description | Glycosyl hydrolases family 31 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH31 | 429 | 917 | 0 |
| LHGGSPTELITSYTESTGRPPALPRWITSGAVVGMQGGTDAVRRVWKQLQDHDVPVSAFWLQDWVGQRKTSIGSQLWWNWEVDDDHYAGWNDLVRDLRRR GVRTMTYCNPCLVPMDKKANARRHLFEEAKKLGLLVRDAAGEPYMMPNTAFDVAMLDFTNPAARAWFKGEVLAVMARGGAAGWMADFGEGLPLNARLHSS GSGDDGPVAAHNRYPELWARVNREFADEWRSGEHRRVADDADDGDGDGELVFFVRAGFRESSRWAMLFWEGDQMVSWQANDGIKSSVVGLLTGGMSGFPL NHGDAGGYCTVDLPLLRYRRSEELLLRWLELSAFTVVFRTHEGNKPGSNCQFYSNNRTLAHFARCAKIYKAWEFYRIQLVEEAAEKGLPVARHLFLHYPE DQRVQKMTYQQFLVGTEMLVVPVLDKGRSTVTAYFPMSDGGLWKHVWTGDEFGGRTSRGGVGEGMSHGSEAEVEARIGFPAVFVRVGST | |||
| Full Sequence |
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| Protein Sequence Length: 933 Download |
| MAPPPPPTPS PAQPKTRKKD HVRLNNPFPR AVPSSSLRHR DAAPPLSFSP SSKLAHGAHD 60 FPVGPHFLLR WDPSLGGAVS LAPRRGGGGA TMWETVPGVA FVSAASVDTE ADECRGSFAL 120 RDGRARLVPD RQRVDRIRAV YRRDADADAD ADLLRVAGAA FQASEHEQAR FPVVVITGVV 180 SARKPTPSPS CLCGRRRAAA AAGRPVLSAR YWILLEEKSD TQVAFRVNLG DYQWSCDHDR 240 HATHPSPLPS PSPSTSPRTH RAGSILRLRL STRVQRSSGG SKKKKKLAFA AAGVPADREE 300 LAPLVPAAAA KEELEFNRVW MTLASSREER FYGFGEQFSR VEFKGKRVPV LVQEQGIGRG 360 DQPITFAANL VSYRSGGNWS TTYAPSPFYM TSKMRSLYLE GYDYSIFDLT KPDRVQIQVY 420 GSSVQGRILH GGSPTELITS YTESTGRPPA LPRWITSGAV VGMQGGTDAV RRVWKQLQDH 480 DVPVSAFWLQ DWVGQRKTSI GSQLWWNWEV DDDHYAGWND LVRDLRRRGV RTMTYCNPCL 540 VPMDKKANAR RHLFEEAKKL GLLVRDAAGE PYMMPNTAFD VAMLDFTNPA ARAWFKGEVL 600 AVMARGGAAG WMADFGEGLP LNARLHSSGS GDDGPVAAHN RYPELWARVN REFADEWRSG 660 EHRRVADDAD DGDGDGELVF FVRAGFRESS RWAMLFWEGD QMVSWQANDG IKSSVVGLLT 720 GGMSGFPLNH GDAGGYCTVD LPLLRYRRSE ELLLRWLELS AFTVVFRTHE GNKPGSNCQF 780 YSNNRTLAHF ARCAKIYKAW EFYRIQLVEE AAEKGLPVAR HLFLHYPEDQ RVQKMTYQQF 840 LVGTEMLVVP VLDKGRSTVT AYFPMSDGGL WKHVWTGDEF GGRTSRGGVG EGMSHGSEAE 900 VEARIGFPAV FVRVGSTVGE RFVRNLRDLK VL* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd06589 | GH31 | 1.0e-20 | 446 | 791 | 349 | + The enzymes of glycosyl hydrolase family 31 (GH31) occur in prokaryotes, eukaryotes, and archaea with a wide range of hydrolytic activities, including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein. In most cases, the pyranose moiety recognized in subsite -1 of the substrate binding site is an alpha-D-glucose, though some GH31 family members show a preference for alpha-D-xylose. Several GH31 enzymes can accommodate both glucose and xylose and different levels of discrimination between the two have been observed. Most characterized GH31 enzymes are alpha-glucosidases. In mammals, GH31 members with alpha-glucosidase activity are implicated in at least three distinct biological processes. The lysosomal acid alpha-glucosidase (GAA) is essential for glycogen degradation and a deficiency or malfunction of this enzyme causes glycogen storage disease II, also known as pompe disease. In the endoplasmic reticulum, alpha-glucosidase II catalyzes the second step in the N-linked oligosaccharide processing pathway that constitutes part of the quality control system for glycoprotein folding and maturation. The intestinal enzymes sucrase-isomaltase (SI) and maltase-glucoamylase (MGAM) play key roles in the final stage of carbohydrate digestion, making alpha-glucosidase inhibitors useful in the treatment of type 2 diabetes. GH31 alpha-glycosidases are retaining enzymes that cleave their substrates via an acid/base-catalyzed, double-displacement mechanism involving a covalent glycosyl-enzyme intermediate. Two aspartic acid residues have been identified as the catalytic nucleophile and the acid/base, respectively. | ||
| pfam01055 | Glyco_hydro_31 | 5.0e-57 | 431 | 918 | 494 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
| COG1501 | COG1501 | 7.0e-67 | 317 | 916 | 620 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
| cd06594 | GH31_glucosidase_YihQ | 3.0e-127 | 446 | 791 | 346 | + YihQ is a bacterial alpha-glucosidase with a conserved glycosyl hydrolase family 31 (GH31) domain that catalyzes the release of an alpha-glucosyl residue from the non-reducing end of alpha-glucoside substrates such as alpha-glucosyl fluoride. Orthologs of YihQ that have not yet been functionally characterized are present in plants and fungi. YihQ has sequence similarity to other GH31 enzymes such as CtsZ, a 6-alpha-glucosyltransferase from Bacillus globisporus, and YicI, an alpha-xylosidase from Echerichia coli. In bacteria, YihQ (along with YihO) is important for bacterial O-antigen capsule assembly and translocation. | ||
| PRK10426 | PRK10426 | 0 | 317 | 930 | 615 | + alpha-glucosidase; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005576 | extracellular region |
| GO:0005618 | cell wall |
| GO:0005773 | vacuole |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD81144.1 | 0 | 1 | 932 | 1 | 932 | alpha-glucosidase -like [Oryza sativa Japonica Group] |
| GenBank | EEC70377.1 | 0 | 374 | 932 | 52 | 610 | hypothetical protein OsI_01324 [Oryza sativa Indica Group] |
| GenBank | EEE54297.1 | 0 | 15 | 665 | 15 | 666 | hypothetical protein OsJ_01231 [Oryza sativa Japonica Group] |
| RefSeq | NP_001042692.1 | 0 | 1 | 490 | 1 | 490 | Os01g0268500 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002308887.1 | 0 | 15 | 932 | 5 | 875 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4kmq_A | 2e-22 | 316 | 930 | 256 | 842 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
| PDB | 4ba0_A | 4e-22 | 304 | 875 | 127 | 641 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
| PDB | 4b9z_A | 4e-22 | 304 | 875 | 127 | 641 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
| PDB | 4b9y_A | 4e-22 | 304 | 875 | 127 | 641 | A Chain A, Crystal Structure Of Apo Agd31b, Alpha-transglucosylase In Glycoside Hydrolase Family 31 |
| PDB | 2f2h_F | 3e-21 | 329 | 882 | 160 | 646 | A Chain A, Structure Of The Yici Thiosugar Michaelis Complex |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-2141 | EC-3.2.1.20 | α-glucosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CB646923 | 248 | 493 | 740 | 0 |
| CO467346 | 279 | 370 | 648 | 0 |
| CB676026 | 249 | 685 | 933 | 0 |
| CB676025 | 269 | 370 | 638 | 0 |
| DV857946 | 258 | 678 | 933 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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