| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os01g51754.1 |
| Family | GH13 |
| Protein Properties | Length: 562 Molecular Weight: 63732.3 Isoelectric Point: 5.4912 |
| Chromosome | Chromosome/Scaffold: 1 Start: 29760719 End: 29770037 |
| Description | alpha-amylase-like 3 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 193 | 481 | 3.7e-38 |
| KAKELSSMGFTIVWSPPPTDSVSPEGYMPRDLYNLNSRYGTMEELKEAVKRFHEAGMKVLGDAVLNHRCAQFQNQNGVWNIFGGRLNWDDRAVVADDPHF QGRGNKSSGDNFHAAPNIDHSQEFVRSDLKEWLCWMRKEVGYDGWRLDFVRGFWGGYVHDYLEASEPYFAVGEYWDSLSYTYGEMDYNQDAHRQRIVDWI NATNGTAGAFDVTTKGILHSALERSEYWRLSDEKGKPPGVLGWWPSRAVTFIENHDTGSTQGHWRFPFGMELQGYVYILTHPGTPAIFY | |||
| Full Sequence |
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| Protein Sequence Length: 562 Download |
| MTWEIPPEPH PPATKIFRQK ALQTMLQQKA DGTGNSLSFL LDGEYSGLIF VVKLDEYTWL 60 RNVENGFDFY IPLTRADAEA DKQKADDKSS QDDGLISDIR NLVVGLSSRR GQRAKNKVLQ 120 EDILQEIERL AAEAYSIFRS PTIDTVEESV YIDDSSIVKP ACSGTGSGFE ILCQGFNWES 180 HKSGKWYVEL GSKAKELSSM GFTIVWSPPP TDSVSPEGYM PRDLYNLNSR YGTMEELKEA 240 VKRFHEAGMK VLGDAVLNHR CAQFQNQNGV WNIFGGRLNW DDRAVVADDP HFQGRGNKSS 300 GDNFHAAPNI DHSQEFVRSD LKEWLCWMRK EVGYDGWRLD FVRGFWGGYV HDYLEASEPY 360 FAVGEYWDSL SYTYGEMDYN QDAHRQRIVD WINATNGTAG AFDVTTKGIL HSALERSEYW 420 RLSDEKGKPP GVLGWWPSRA VTFIENHDTG STQGHWRFPF GMELQGYVYI LTHPGTPAIF 480 YDHIFSHLQP EIAKLISIRN RQKIHCRSKI KILKAEGNLY AAEIDERVTM KIGAGHFEPS 540 GPTNWVVAAE GQDYKVWEVS S* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 1.0e-47 | 170 | 501 | 419 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN00196 | PLN00196 | 3.0e-137 | 170 | 558 | 406 | + alpha-amylase; Provisional | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 5.0e-160 | 171 | 510 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02361 | PLN02361 | 1.0e-170 | 167 | 558 | 398 | + alpha-amylase | ||
| PLN02784 | PLN02784 | 0 | 1 | 560 | 577 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004556 | alpha-amylase activity |
| GO:0005509 | calcium ion binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAX33231.1 | 0 | 3 | 560 | 329 | 901 | plastid alpha-amylase [Malus x domestica] |
| DDBJ | BAG89388.1 | 0 | 1 | 561 | 1 | 561 | unnamed protein product [Oryza sativa Japonica Group] |
| GenBank | EEC71386.1 | 0 | 1 | 561 | 316 | 876 | hypothetical protein OsI_03507 [Oryza sativa Indica Group] |
| RefSeq | NP_001044062.1 | 0 | 1 | 561 | 316 | 876 | Os01g0715400 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002456247.1 | 0 | 1 | 561 | 251 | 820 | hypothetical protein SORBIDRAFT_03g032830 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3bsg_A | 0 | 170 | 558 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| PDB | 2qps_A | 0 | 170 | 558 | 2 | 403 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1rpk_A | 0 | 170 | 558 | 2 | 403 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1p6w_A | 0 | 170 | 558 | 2 | 403 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1ht6_A | 0 | 170 | 558 | 2 | 403 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
| starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EG631183 | 576 | 3 | 560 | 0 |
| HO826981 | 403 | 159 | 560 | 0 |
| GO885960 | 287 | 256 | 542 | 0 |
| DV475507 | 279 | 181 | 459 | 0 |
| DR932783 | 288 | 170 | 457 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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