| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os01g51754.3 |
| Family | CBM45 |
| Protein Properties | Length: 420 Molecular Weight: 46653.5 Isoelectric Point: 4.8783 |
| Chromosome | Chromosome/Scaffold: 1 Start: 29762378 End: 29766397 |
| Description | alpha-amylase-like 3 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM45 | 169 | 245 | 1.9e-23 |
| IHWGVCKDNTMTWEIPPEPHPPATKIFRQKALQTMLQQKADGTGNSLSFLLDGEYSGLIFVVKLDEYTWLRNVENGF | |||
| Full Sequence |
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| Protein Sequence Length: 420 Download |
| MRPPGSVPIK DYAIETSLDT PHNSEGKTIH EVQIKIDKGT SIAAINFVLK EEETGAWFQH 60 KGQDFRIPLS GSFGGDLLGT EQDIDVRPGA LGHLSNVLQK PEGPIAEPHK TVPDDKGSRT 120 KHISGFYEEY PILKTVYVQN FITVNVRENN GTTKHAVEFD TDIPGEVIIH WGVCKDNTMT 180 WEIPPEPHPP ATKIFRQKAL QTMLQQKADG TGNSLSFLLD GEYSGLIFVV KLDEYTWLRN 240 VENGFDFYIP LTRADAEADK QKADDKSSQD DGLISDIRNL VVGLSSRRGQ RAKNKVLQED 300 ILQEIERLAA EAYSIFRSPT IDTVEESVYI DDSSIVKPAC SGTGSGFEIL CQGFNWESHK 360 SGKWYVELGS KAKELSSMGF TIVWSPPPTD SVSPEGYMPR DLYNLNSRYI SVCTIIVLL* 420 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02784 | PLN02784 | 5.0e-8 | 126 | 251 | 132 | + alpha-amylase | ||
| PLN00196 | PLN00196 | 2.0e-13 | 348 | 409 | 64 | + alpha-amylase; Provisional | ||
| PLN02361 | PLN02361 | 4.0e-21 | 345 | 411 | 67 | + alpha-amylase | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 5.0e-25 | 349 | 409 | 63 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02784 | PLN02784 | 0 | 1 | 409 | 429 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| GO:0009056 | catabolic process |
| GO:0009536 | plastid |
| GO:0009987 | cellular process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EEC71386.1 | 0 | 1 | 409 | 138 | 546 | hypothetical protein OsI_03507 [Oryza sativa Indica Group] |
| RefSeq | NP_001044062.1 | 0 | 1 | 409 | 138 | 546 | Os01g0715400 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002456247.1 | 0 | 1 | 412 | 79 | 493 | hypothetical protein SORBIDRAFT_03g032830 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3bsh_A | 0.000000000008 | 348 | 419 | 2 | 75 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) Double Mutant Y105aY380A IN COMPLEX WITH INHIBITOR ACARBOSE |
| PDB | 3bsg_A | 0.00000000001 | 348 | 419 | 2 | 75 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| PDB | 2qps_A | 0.00000000001 | 348 | 419 | 2 | 75 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1rpk_A | 0.00000000001 | 348 | 419 | 2 | 75 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| PDB | 1p6w_A | 0.00000000001 | 348 | 419 | 2 | 75 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GO876675 | 312 | 100 | 403 | 0 |
| HX129171 | 253 | 126 | 370 | 0 |
| EG631183 | 431 | 1 | 409 | 0 |
| GR315677 | 260 | 129 | 380 | 0 |
| EG631183 | 142 | 120 | 251 | 0.067 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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