| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os01g53000.2 |
| Family | GT20 |
| Protein Properties | Length: 787 Molecular Weight: 89555.1 Isoelectric Point: 6.9157 |
| Chromosome | Chromosome/Scaffold: 1 Start: 30460007 End: 30463589 |
| Description | trehalose-phosphatase/synthase 7 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 74 | 562 | 0 |
| LASDRLIVVANTLPVRCERRPDGRGWSFCWDEDSLLLHLRDGLPDDMEVLYVGSLRADVPSAEQDDVAQALLDRFRCVPAFLPKDVLDRFYHGFCKQTLW PLFHYMLPFTSDHGGRFDRSQWEAYVLANKLFSQRVIEVLNPEDDYIWIHDYHLLALPSFLRRRFNRLRIGFFLHSPFPSSELYRSLPVRDEILKSLLNC DLIGFHTFDYARHFLSCCSRMLGIEYQSKRGYIGLDYFGRTVGIKIMPVGINMTQLQTQIRLPDLEWRVAELRKQFDGKTVMLGVDDMDIFKGINLKVLA FEQMLRTHPKWQRKAVLVQIANPRGGGGKDLEEIQAEIDESCRRINAQFSRPGYVPVVIINRALSSVERMAYYTVAECVVVTAVRDGMNLTPYEYIVCRQ GFPDLDGSGDDGPRRKSMLVVSEFIGCSPSLSGAIRVNPWNIDTTAEAMNESIALSENEKQLRHEKHYRYVSSHDVAYWSKSYIHDLER | |||
| Full Sequence |
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| Protein Sequence Length: 787 Download |
| MFSRSYTNLV DLANGNLSAL DYGGGGGGGG GGNGAGGRPP RARRMQRTMT TPGTLAELDE 60 ERAGSVTSDV PSSLASDRLI VVANTLPVRC ERRPDGRGWS FCWDEDSLLL HLRDGLPDDM 120 EVLYVGSLRA DVPSAEQDDV AQALLDRFRC VPAFLPKDVL DRFYHGFCKQ TLWPLFHYML 180 PFTSDHGGRF DRSQWEAYVL ANKLFSQRVI EVLNPEDDYI WIHDYHLLAL PSFLRRRFNR 240 LRIGFFLHSP FPSSELYRSL PVRDEILKSL LNCDLIGFHT FDYARHFLSC CSRMLGIEYQ 300 SKRGYIGLDY FGRTVGIKIM PVGINMTQLQ TQIRLPDLEW RVAELRKQFD GKTVMLGVDD 360 MDIFKGINLK VLAFEQMLRT HPKWQRKAVL VQIANPRGGG GKDLEEIQAE IDESCRRINA 420 QFSRPGYVPV VIINRALSSV ERMAYYTVAE CVVVTAVRDG MNLTPYEYIV CRQGFPDLDG 480 SGDDGPRRKS MLVVSEFIGC SPSLSGAIRV NPWNIDTTAE AMNESIALSE NEKQLRHEKH 540 YRYVSSHDVA YWSKSYIHDL ERSCRDHFRR RCWGIGLGFG FRVVALDRNF KKLTVDSIVT 600 DYKNSKSRVI LLDYDGTLVP QTTINRTPNE SVVKIMNALC DDKKNVVFIV SGRGRDSLEK 660 WFSPCQDLGI AAEHGYFMRW TRDEQWQLNN QCSEFGWMQM AKPVMNLYTE ATDGSYIETK 720 ESALVWHHQD ADPGFGSSQA KEMLDHLESV LANEPVCVKS GQQIVEVKPQ VFHFSHIIHL 780 LLWCWH* 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 4.0e-150 | 78 | 769 | 714 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN02205 | PLN02205 | 0 | 1 | 770 | 777 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 78 | 562 | 489 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| cd03788 | GT1_TPS | 0 | 78 | 561 | 484 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| PRK14501 | PRK14501 | 0 | 78 | 773 | 700 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005829 | cytosol |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005992 | trehalose biosynthetic process |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EAZ13427.1 | 0 | 1 | 770 | 1 | 770 | hypothetical protein OsJ_03344 [Oryza sativa Japonica Group] |
| GenBank | EEC71425.1 | 0 | 1 | 770 | 1 | 715 | hypothetical protein OsI_03616 [Oryza sativa Indica Group] |
| RefSeq | NP_001044141.1 | 0 | 1 | 770 | 1 | 770 | Os01g0730300 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002456290.1 | 0 | 1 | 771 | 1 | 783 | hypothetical protein SORBIDRAFT_03g033590 [Sorghum bicolor] |
| RefSeq | XP_002458490.1 | 0 | 1 | 770 | 1 | 785 | hypothetical protein SORBIDRAFT_03g034640 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1gz5_D | 0 | 160 | 562 | 72 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_C | 0 | 160 | 562 | 72 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_B | 0 | 160 | 562 | 72 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 1gz5_A | 0 | 160 | 562 | 72 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| PDB | 2wtx_D | 0 | 160 | 562 | 73 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 678 | 99 | 770 | 0 |
| HO779327 | 513 | 119 | 628 | 0 |
| HO779327 | 158 | 625 | 782 | 0 |
| GT051541 | 300 | 462 | 761 | 0 |
| HO777775 | 96 | 1 | 95 | 0.1 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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