| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os01g54560.1 |
| Family | GT20 |
| Protein Properties | Length: 914 Molecular Weight: 102270 Isoelectric Point: 5.2899 |
| Chromosome | Chromosome/Scaffold: 1 Start: 31373407 End: 31377545 |
| Description | trehalose-phosphatase/synthase 7 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 76 | 579 | 0 |
| AGDRVIVVSNQLPVVARRRPDGRGWSFSWDDDSLLLQLRDGIPDEMEVFFVGSLRAEIPVADQEEVSQALLDRFRCAPVFLPDPLNERFYHRFCKRHLWP LFHYMLPFSSSASPSPSSSSSSSSSPSSSSGSGHFDRGAWEAYVLANKFFFEKVVEVINPEDDYVWVHDYHLMALPTFLRRRFNRLRIGFFLHSPFPSSE IYRTLPVREEILKALLNCDLIGFHTFDYARHFLSCCSRMLGIEYQSKRGYIGLDYFGRTVGIKIMPVGVHMGQLKTVLSLPDREWRVSELQQQFEGKTVL LGVDDMDIFKGINLKLLAFENMLRTHPKWQGRAVLVQIANPARGKGKDLEAIQAEIHESCKRINGEFGQSGYSPVVFIDRDVSSVEKIAYYTIAECVVVT AVRDGMNLTPYEYIVCRQGSDSTSEVNGPKKSMLVVSEFIGCSPSLSGAIRVNPWNIEATAEALNEAISMSEQEKHLRHEKHYRYVSTHDVAYWSKSFIQ DLER | |||
| Full Sequence |
|---|
| Protein Sequence Length: 914 Download |
| MMSRSYTNLL DLAEGNFAAL GPGGGGGGRR RSGSFGLKRM SRVMTVPGTL SELDGEDDSE 60 HAATNSVASD VPSSVAGDRV IVVSNQLPVV ARRRPDGRGW SFSWDDDSLL LQLRDGIPDE 120 MEVFFVGSLR AEIPVADQEE VSQALLDRFR CAPVFLPDPL NERFYHRFCK RHLWPLFHYM 180 LPFSSSASPS PSSSSSSSSS PSSSSGSGHF DRGAWEAYVL ANKFFFEKVV EVINPEDDYV 240 WVHDYHLMAL PTFLRRRFNR LRIGFFLHSP FPSSEIYRTL PVREEILKAL LNCDLIGFHT 300 FDYARHFLSC CSRMLGIEYQ SKRGYIGLDY FGRTVGIKIM PVGVHMGQLK TVLSLPDREW 360 RVSELQQQFE GKTVLLGVDD MDIFKGINLK LLAFENMLRT HPKWQGRAVL VQIANPARGK 420 GKDLEAIQAE IHESCKRING EFGQSGYSPV VFIDRDVSSV EKIAYYTIAE CVVVTAVRDG 480 MNLTPYEYIV CRQGSDSTSE VNGPKKSMLV VSEFIGCSPS LSGAIRVNPW NIEATAEALN 540 EAISMSEQEK HLRHEKHYRY VSTHDVAYWS KSFIQDLERA CKDHFRRTCW GIGLGFGFRV 600 VALDPHFTKL NMDSIVMAYE RSESRAIFLD YDGTLVPQTS ISRTPSAEVL RIINTLCSDR 660 RNKVFLVSGR RRDKLGEWFS SCPDLGIAAE HGYFLRWTRD EEWQTCTQTS DFGWMEMAKP 720 VMNLYTEATD GSYIDPKESA LVWHHQDADP GFGSSQAKEL LDHLESVLAN EPVSVKSGQF 780 IVEVKPQGVS KGVVAEKILV SMKERGKQAD FVLCIGDDRS DEDMFENIAD TIKKGMVATN 840 TSLFACTVGQ KPSKAKFYLD DTFEVVTMLS ALADATEPEP ETDLTDEFDE LAVSVSSVDI 900 DDEQTPSDKL IGG* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 2.0e-163 | 79 | 873 | 832 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN02205 | PLN02205 | 0 | 1 | 873 | 882 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 79 | 579 | 505 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 79 | 873 | 801 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| cd03788 | GT1_TPS | 0 | 79 | 578 | 500 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005829 | cytosol |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005992 | trehalose biosynthetic process |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACN25869.1 | 0 | 40 | 913 | 1 | 873 | unknown [Zea mays] |
| GenBank | ACN33296.1 | 0 | 1 | 913 | 1 | 911 | unknown [Zea mays] |
| GenBank | EEC71477.1 | 0 | 1 | 913 | 1 | 914 | hypothetical protein OsI_03737 [Oryza sativa Indica Group] |
| RefSeq | NP_001044248.1 | 0 | 1 | 913 | 1 | 913 | Os01g0749400 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002458490.1 | 0 | 1 | 913 | 1 | 909 | hypothetical protein SORBIDRAFT_03g034640 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 162 | 579 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 162 | 579 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 162 | 579 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 162 | 579 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 162 | 579 | 73 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 783 | 100 | 873 | 0 |
| HO779327 | 532 | 120 | 646 | 0 |
| HO779327 | 232 | 642 | 873 | 0 |
| HO795511 | 465 | 412 | 873 | 0 |
| HO777775 | 96 | 1 | 96 | 1.1 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
