| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os01g54560.2 |
| Family | GT20 |
| Protein Properties | Length: 885 Molecular Weight: 99367.5 Isoelectric Point: 5.1732 |
| Chromosome | Chromosome/Scaffold: 1 Start: 31373407 End: 31377545 |
| Description | trehalose-phosphatase/synthase 7 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 47 | 550 | 0 |
| AGDRVIVVSNQLPVVARRRPDGRGWSFSWDDDSLLLQLRDGIPDEMEVFFVGSLRAEIPVADQEEVSQALLDRFRCAPVFLPDPLNERFYHRFCKRHLWP LFHYMLPFSSSASPSPSSSSSSSSSPSSSSGSGHFDRGAWEAYVLANKFFFEKVVEVINPEDDYVWVHDYHLMALPTFLRRRFNRLRIGFFLHSPFPSSE IYRTLPVREEILKALLNCDLIGFHTFDYARHFLSCCSRMLGIEYQSKRGYIGLDYFGRTVGIKIMPVGVHMGQLKTVLSLPDREWRVSELQQQFEGKTVL LGVDDMDIFKGINLKLLAFENMLRTHPKWQGRAVLVQIANPARGKGKDLEAIQAEIHESCKRINGEFGQSGYSPVVFIDRDVSSVEKIAYYTIAECVVVT AVRDGMNLTPYEYIVCRQGSDSTSEVNGPKKSMLVVSEFIGCSPSLSGAIRVNPWNIEATAEALNEAISMSEQEKHLRHEKHYRYVSTHDVAYWSKSFIQ DLER | |||
| Full Sequence |
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| Protein Sequence Length: 885 Download |
| MMSGSFGLKR MSRVMTVPGT LSELDGEDDS EHAATNSVAS DVPSSVAGDR VIVVSNQLPV 60 VARRRPDGRG WSFSWDDDSL LLQLRDGIPD EMEVFFVGSL RAEIPVADQE EVSQALLDRF 120 RCAPVFLPDP LNERFYHRFC KRHLWPLFHY MLPFSSSASP SPSSSSSSSS SPSSSSGSGH 180 FDRGAWEAYV LANKFFFEKV VEVINPEDDY VWVHDYHLMA LPTFLRRRFN RLRIGFFLHS 240 PFPSSEIYRT LPVREEILKA LLNCDLIGFH TFDYARHFLS CCSRMLGIEY QSKRGYIGLD 300 YFGRTVGIKI MPVGVHMGQL KTVLSLPDRE WRVSELQQQF EGKTVLLGVD DMDIFKGINL 360 KLLAFENMLR THPKWQGRAV LVQIANPARG KGKDLEAIQA EIHESCKRIN GEFGQSGYSP 420 VVFIDRDVSS VEKIAYYTIA ECVVVTAVRD GMNLTPYEYI VCRQGSDSTS EVNGPKKSML 480 VVSEFIGCSP SLSGAIRVNP WNIEATAEAL NEAISMSEQE KHLRHEKHYR YVSTHDVAYW 540 SKSFIQDLER ACKDHFRRTC WGIGLGFGFR VVALDPHFTK LNMDSIVMAY ERSESRAIFL 600 DYDGTLVPQT SISRTPSAEV LRIINTLCSD RRNKVFLVSG RRRDKLGEWF SSCPDLGIAA 660 EHGYFLRWTR DEEWQTCTQT SDFGWMEMAK PVMNLYTEAT DGSYIDPKES ALVWHHQDAD 720 PGFGSSQAKE LLDHLESVLA NEPVSVKSGQ FIVEVKPQGV SKGVVAEKIL VSMKERGKQA 780 DFVLCIGDDR SDEDMFENIA DTIKKGMVAT NTSLFACTVG QKPSKAKFYL DDTFEVVTML 840 SALADATEPE PETDLTDEFD ELAVSVSSVD IDDEQTPSDK LIGG* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 1.0e-163 | 50 | 844 | 832 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN02205 | PLN02205 | 0 | 3 | 844 | 853 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 50 | 550 | 505 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 50 | 844 | 801 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| cd03788 | GT1_TPS | 0 | 50 | 549 | 500 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005829 | cytosol |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005992 | trehalose biosynthetic process |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACN25869.1 | 0 | 11 | 884 | 1 | 873 | unknown [Zea mays] |
| GenBank | ACN33296.1 | 0 | 4 | 884 | 35 | 911 | unknown [Zea mays] |
| GenBank | EEC71477.1 | 0 | 6 | 884 | 35 | 914 | hypothetical protein OsI_03737 [Oryza sativa Indica Group] |
| RefSeq | NP_001044248.1 | 0 | 6 | 884 | 35 | 913 | Os01g0749400 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002458490.1 | 0 | 4 | 884 | 32 | 909 | hypothetical protein SORBIDRAFT_03g034640 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 133 | 550 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 133 | 550 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 133 | 550 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 133 | 550 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 133 | 550 | 73 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 783 | 71 | 844 | 0 |
| HO779327 | 532 | 91 | 617 | 0 |
| HO779327 | 232 | 613 | 844 | 0 |
| HO795511 | 465 | 383 | 844 | 0 |
| HO797286 | 465 | 385 | 844 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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