| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os01g65780.2 |
| Family | GT8 |
| Protein Properties | Length: 636 Molecular Weight: 73391.9 Isoelectric Point: 8.5563 |
| Chromosome | Chromosome/Scaffold: 1 Start: 38197552 End: 38202617 |
| Description | plant glycogenin-like starch initiation protein 1 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 294 | 518 | 3.60134e-43 |
| AKARLYSVDRRREAYATILHSASEYVCGAITAAQSIRQAGSTRDFVILVDETISNHHRKGLEAAGWKVRIIQRIRNPKAERDAYNEWNYSKFRLWQLTDY DKIIFIDADLLILRNVDFLFAMPEITATGNNATLFNSGVMVIEPSNCTFQLLMDHINEITSYNGGDQGYLNEIFTWWHRIPKHMNFLKHFWEGDEEEVKV KKTRLFGADPPILYVLHYLGLKPWL | |||
| Full Sequence |
|---|
| Protein Sequence Length: 636 Download |
| MGSLETTNTR YRPAGAADDT AKRRTQKSKS FKEVEKFDVF VLEKSSGCKF RSLQLLLFAI 60 MSAAFLTLLY TPSVYDHQMQ SSSRFVSGWI WDKTIPDPRY VSSLGVQWED VYKTVENLND 120 GERKLKVGLL NFNSTEIGSW TQLLPDSDFS IIRLEHAKES ITWQTLYPEW IDEEEETEIP 180 SCPSLPDPIF PRGTHFDVVA VKLPCTRAGG WSRDVARLHL QLSAAKVAVT ASRGNRGIHV 240 LFVTDCFPIP NLFSCKNLVK HEGNAWMYKP DLKALREKLR LPVGSCELAV PLKAKARLYS 300 VDRRREAYAT ILHSASEYVC GAITAAQSIR QAGSTRDFVI LVDETISNHH RKGLEAAGWK 360 VRIIQRIRNP KAERDAYNEW NYSKFRLWQL TDYDKIIFID ADLLILRNVD FLFAMPEITA 420 TGNNATLFNS GVMVIEPSNC TFQLLMDHIN EITSYNGGDQ GYLNEIFTWW HRIPKHMNFL 480 KHFWEGDEEE VKVKKTRLFG ADPPILYVLH YLGLKPWLCF RDYDCNWNNP ILREFASDVA 540 HARWWKVHDK MPKKLQHYCL LRSRQKAGLE WDRRQAEKAN FTDGHWRRNI TDPRLKTCFE 600 KFCFWESMLW HWGESKNSTK ENPVPATPTA SLTSS* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00505 | Glyco_transf_8 | 4.0e-11 | 311 | 517 | 248 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| cd06914 | GT8_GNT1 | 4.0e-18 | 308 | 468 | 171 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| PLN00176 | PLN00176 | 2.0e-18 | 287 | 517 | 274 | + galactinol synthase | ||
| pfam01501 | Glyco_transf_8 | 2.0e-39 | 309 | 519 | 254 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 1.0e-72 | 306 | 551 | 256 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005794 | Golgi apparatus |
| GO:0005975 | carbohydrate metabolic process |
| GO:0009058 | biosynthetic process |
| GO:0009987 | cellular process |
| GO:0016740 | transferase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAG90458.1 | 0 | 61 | 635 | 1 | 575 | unnamed protein product [Oryza sativa Japonica Group] |
| GenBank | EAZ14371.1 | 0 | 17 | 635 | 33 | 651 | hypothetical protein OsJ_04291 [Oryza sativa Japonica Group] |
| GenBank | EEC71899.1 | 0 | 1 | 635 | 1 | 635 | hypothetical protein OsI_04661 [Oryza sativa Indica Group] |
| RefSeq | NP_001044991.1 | 0 | 1 | 635 | 1 | 635 | Os01g0880200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002456736.1 | 0 | 1 | 635 | 1 | 633 | hypothetical protein SORBIDRAFT_03g041660 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3u2t_A | 1e-22 | 297 | 517 | 17 | 242 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
| PDB | 3u2x_B | 2e-22 | 306 | 517 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
| PDB | 3u2x_A | 2e-22 | 306 | 517 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
| PDB | 3u2v_B | 2e-22 | 306 | 517 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
| PDB | 3u2v_A | 2e-22 | 306 | 517 | 5 | 221 | A Chain A, Crystal Structure Of Human Glycogenin-1 (Gyg1) Complexed With Manganese |
