| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os01g73580.1 |
| Family | GH32 |
| Protein Properties | Length: 513 Molecular Weight: 57609 Isoelectric Point: 5.9572 |
| Chromosome | Chromosome/Scaffold: 1 Start: 42626321 End: 42630440 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 1 | 303 | 0 |
| MYYNGIYHEFYQYNPNGSVWGNIVWGHSVSTDLINWIRLEPAIEGNTPSDINGCWTGSATILTGDQPVIIYTGADTEKRQVQNIVLPKNRSDPYLREWTK PKNNPLIEPVGPGLNSNQFRDPTTGWIGPDGLWRIAVGAELNGYSAALLYKSKDFMQWTRVDHPLYSSNASNMWECPDFFAVLPGKNNGLDLSAAIPNGA KHVLKMSLDSCDKYMIGVYDLKHDMFVPDTVLDDRRLWLRIDYGNYYASKSFFDSKKGRRIIWGWTNETDSTSDDVAKGWAGIHAIPRTIWLDGDGKRLL QWP | |||
| Full Sequence |
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| Protein Sequence Length: 513 Download |
| MYYNGIYHEF YQYNPNGSVW GNIVWGHSVS TDLINWIRLE PAIEGNTPSD INGCWTGSAT 60 ILTGDQPVII YTGADTEKRQ VQNIVLPKNR SDPYLREWTK PKNNPLIEPV GPGLNSNQFR 120 DPTTGWIGPD GLWRIAVGAE LNGYSAALLY KSKDFMQWTR VDHPLYSSNA SNMWECPDFF 180 AVLPGKNNGL DLSAAIPNGA KHVLKMSLDS CDKYMIGVYD LKHDMFVPDT VLDDRRLWLR 240 IDYGNYYASK SFFDSKKGRR IIWGWTNETD STSDDVAKGW AGIHAIPRTI WLDGDGKRLL 300 QWPIEEVESL RRNEVSHQGL ELKKGDLFEI KGTDTLQADV EIDFELTSID AADPFDPSWL 360 LDTEKHCREA DASVHGGLGP FGLVVLASDN MDEHTTVHFR VYKSEQKYMV LLCSDLRRSS 420 LRPGLYTPAY GGFFEYDLEK EKKISLRTLI DRSAVESFGG GGRACIMARV YPAAVVDGAT 480 HMYAFNNGSS TVKVSQLKAW SMTRAQVNVR KG* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 3.0e-41 | 2 | 473 | 498 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 5.0e-53 | 1 | 480 | 499 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 1.0e-78 | 1 | 306 | 320 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 8.0e-123 | 1 | 303 | 313 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 1.0e-139 | 1 | 462 | 473 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005618 | cell wall |
| GO:0006950 | response to stress |
| GO:0008152 | metabolic process |
| GO:0009628 | response to abiotic stimulus |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAZ29514.1 | 0 | 1 | 506 | 63 | 569 | fructan exohydrolase [Lolium perenne] |
| GenBank | ABY64749.1 | 0 | 1 | 512 | 69 | 580 | fructan 6-exohydrolase [Lolium perenne] |
| DDBJ | BAD05180.1 | 0 | 1 | 512 | 79 | 590 | cell wall invertase [Oryza sativa Japonica Group] |
| GenBank | EEC72233.1 | 0 | 1 | 512 | 75 | 586 | hypothetical protein OsI_05346 [Oryza sativa Indica Group] |
| RefSeq | NP_001045504.1 | 0 | 1 | 512 | 79 | 590 | Os01g0966700 [Oryza sativa (japonica cultivar-group)] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 1 | 508 | 28 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2qqw_A | 0 | 1 | 508 | 24 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 1 | 508 | 24 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 1 | 508 | 24 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 1 | 508 | 24 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| fructan degradation | RXN-1841 | EC-3.2.1.80 | fructan β-fructosidase |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GT040156 | 334 | 36 | 369 | 0 |
| GO841347 | 325 | 119 | 443 | 0 |
| GT037276 | 319 | 142 | 460 | 0 |
| CB676546 | 257 | 131 | 387 | 0 |
| FG552225 | 270 | 47 | 316 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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