| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os02g14090.1 |
| Family | AA7 |
| Protein Properties | Length: 568 Molecular Weight: 62117.8 Isoelectric Point: 6.7444 |
| Chromosome | Chromosome/Scaffold: 2 Start: 7690242 End: 7692254 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 85 | 315 | 0 |
| PRTPRPALVLTPVTADEVRACVVCCRAHGLTVRARSGGHDYEGLSYRSLGRSPRFAVVDVAALRAVRVDAARGVARAEAGATLGELYYAVAEGSGGRLGF PAGICPTVCVGGHLSGGGFGPMMRKYGLAADNVVDAEVVDAEGRLLDRAAMGEGLFWAIRGGGGGSVGIVVSWTVNLVPVPAVVSAFTVHRLLLRRGGHD EQSLLRLLTKWQAVAHALPDNLFVKMSMEAK | |||
| Full Sequence |
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| Protein Sequence Length: 568 Download |
| MEPSRPRLPL LLLLLLHFSL LASPCSARWR DGCGVGDLRE SFLRCVARRS PATAADPSRL 60 VHAPGDASYP SLLDSTIQNL RFASPRTPRP ALVLTPVTAD EVRACVVCCR AHGLTVRARS 120 GGHDYEGLSY RSLGRSPRFA VVDVAALRAV RVDAARGVAR AEAGATLGEL YYAVAEGSGG 180 RLGFPAGICP TVCVGGHLSG GGFGPMMRKY GLAADNVVDA EVVDAEGRLL DRAAMGEGLF 240 WAIRGGGGGS VGIVVSWTVN LVPVPAVVSA FTVHRLLLRR GGHDEQSLLR LLTKWQAVAH 300 ALPDNLFVKM SMEAKTINDG DDSTRHPLVT FKSLFLGNCS DMITQIDHHL PELGIKPTDC 360 REMNWLQSML YSYGYTNGQP AEVLLDRTLQ PKDYYKIKLD YLTSPIPTPG LIELLTKIVE 420 DEDGSIDIDP QGGEMSRIPE SGTPYAHRSG YLYNLQYFVK WGGDKNVSYE DDHLSWVRGL 480 HELMTPYVSK NPRAAYINYR DLDLGQNVEG NTSYEEARVW GEKYFRGNFR RLAMVKGEVD 540 PDQLFWSEQS IPPLVVSTRD AGLVSDS* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 1.0e-6 | 83 | 212 | 134 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 6.0e-15 | 90 | 231 | 142 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam08031 | BBE | 3.0e-16 | 495 | 552 | 58 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003674 | molecular_function |
| GO:0003824 | catalytic activity |
| GO:0005488 | binding |
| GO:0005829 | cytosol |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACN34780.1 | 0 | 22 | 567 | 25 | 561 | unknown [Zea mays] |
| GenBank | EAY85134.1 | 0 | 1 | 567 | 1 | 566 | hypothetical protein OsI_06489 [Oryza sativa Indica Group] |
| GenBank | EAZ22357.1 | 0 | 120 | 567 | 63 | 510 | hypothetical protein OsJ_06016 [Oryza sativa Japonica Group] |
| RefSeq | NP_001046387.1 | 0 | 1 | 553 | 1 | 553 | Os02g0235900 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002451843.1 | 0 | 22 | 546 | 21 | 548 | hypothetical protein SORBIDRAFT_04g008542 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4dns_B | 0 | 37 | 555 | 8 | 497 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 4dns_A | 0 | 37 | 555 | 8 | 497 | A Chain A, Crystal Structure Of Bermuda Grass Isoallergen Bg60 Provides Insight Into The Various Cross-Allergenicity Of The Pollen Group 4 Allergens |
| PDB | 3vte_A | 0 | 39 | 554 | 4 | 513 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_B | 0 | 39 | 555 | 8 | 497 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| PDB | 3tsj_A | 0 | 39 | 555 | 8 | 497 | A Chain A, Crystal Structure Of Tetrahydrocannabinolic Acid Synthase From Cannabis Sativa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| cannabinoid biosynthesis | RXN-7854 | EC-1.21.3 | tetrahydrocannabinolic acid synthase |
| Signal Peptide | |||||
|---|---|---|---|---|---|
| Cleavage Site | |||||
| 27 | |||||
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CI148141 | 176 | 393 | 568 | 0 |
| EG385139 | 205 | 355 | 559 | 0 |
| CI197644 | 173 | 396 | 568 | 0 |
| HX128163 | 206 | 367 | 566 | 0 |
| DV514342 | 317 | 234 | 550 | 0 |
| Orthologous Group | |||||
|---|---|---|---|---|---|
| Species | ID | ||||
| Brachypodium distachyon | Bradi3g35067.1 | ||||
| Panicum virgatum | Pavirv00060976m | ||||
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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