| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os02g32660.1 |
| Family | GH13 |
| Protein Properties | Length: 826 Molecular Weight: 92757.2 Isoelectric Point: 5.9911 |
| Chromosome | Chromosome/Scaffold: 2 Start: 19355748 End: 19367127 |
| Description | starch branching enzyme 2.2 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 340 | 660 | 3.3e-32 |
| LPRIKKLGYNAVQIMAIQEHAYYGSFGYHVTNFFAPSSRFGTPEDLKSLIDKAHELGLVVLMDVVHSHASNNTLDGLNGFDGTDTHYFHSGSRGHHWMWD SRLFNYGNWEVLRFLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVAFTGNYSEYFGFATDADAVVYLMLVNDLIHGLYPEAITIGEDVSGMPTFALP VQDGGVGFDYRLHMAVPDKWIELLKQSDESWKMGDIVHTLTNRRWSEKCVTYAESHDQALVGDKTIAFWLMDKDMYDFMALDRPATPSIDRGIALHKMIR LITMGLGGEGYLNFMGNEFGH | |||
| Full Sequence |
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| Protein Sequence Length: 826 Download |
| MAAPASAVPG SAAGLRAGAV RFPVPAGARS WRAAAELPTS RSLLSGRRFP GAVRVGGSGG 60 RVAVRAAGAS GEVMIPEGES DGMPVSAGSD DLQLPALDDE LSTEVGAEVE IESSGASDVE 120 GVKRVVEELA AEQKPRVVPP TGDGQKIFQM DSMLNGYKYH LEYRYSLYRR LRSDIDQYEG 180 GLETFSRGYE KFGFNHSAEG VTYREWAPGA HSAALVGDFN NWNPNADRMS KNEFGVWEIF 240 LPNNADGSSP IPHGSRVKVR METPSGIKDS IPAWIKYSVQ AAGEIPYNGI YYDPPEEEKY 300 IFKHPQPKRP KSLRIYETHV GMSSTEPKIN TYANFRDEVL PRIKKLGYNA VQIMAIQEHA 360 YYGSFGYHVT NFFAPSSRFG TPEDLKSLID KAHELGLVVL MDVVHSHASN NTLDGLNGFD 420 GTDTHYFHSG SRGHHWMWDS RLFNYGNWEV LRFLLSNARW WLEEYKFDGF RFDGVTSMMY 480 THHGLQVAFT GNYSEYFGFA TDADAVVYLM LVNDLIHGLY PEAITIGEDV SGMPTFALPV 540 QDGGVGFDYR LHMAVPDKWI ELLKQSDESW KMGDIVHTLT NRRWSEKCVT YAESHDQALV 600 GDKTIAFWLM DKDMYDFMAL DRPATPSIDR GIALHKMIRL ITMGLGGEGY LNFMGNEFGH 660 PEWIDFPRAP QVLPNGKFIP GNNNSYDKCR RRFDLGDADY LRYRGMLEFD RAMQSLEEKY 720 GFMTSDHQYI SRKHEEDKMI IFEKGDLVFV FNFHWSNSYF DYRVGCLKPG KYKVVLDSDA 780 GLFGGFGRIH HTAEHFTADC SHDNRPYSFS VYSPSRTCVV YAPAE* 840 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02960 | PLN02960 | 6.0e-8 | 182 | 241 | 66 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 1.0e-135 | 251 | 821 | 579 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 72 | 825 | 758 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 296 | 711 | 417 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 251 | 821 | 577 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| GO:0009058 | biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAW80631.1 | 0 | 23 | 823 | 19 | 834 | starch branching enzyme IIb [Triticum aestivum] |
| GenBank | ACE88265.1 | 0 | 1 | 825 | 1 | 825 | rice starch branching enzyme [Oryza sativa Japonica Group] |
| GenBank | EAY86110.1 | 0 | 1 | 825 | 1 | 825 | hypothetical protein OsI_07480 [Oryza sativa Indica Group] |
| RefSeq | NP_001047009.1 | 0 | 1 | 825 | 1 | 825 | Os02g0528200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002453926.1 | 0 | 5 | 825 | 3 | 803 | hypothetical protein SORBIDRAFT_04g021540 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 143 | 821 | 9 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3aml_A | 0 | 143 | 821 | 9 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 143 | 821 | 9 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 143 | 821 | 9 | 690 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 1m7x_D | 4.06377e-44 | 184 | 819 | 6 | 610 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO794536 | 684 | 142 | 825 | 0 |
| HO777638 | 629 | 197 | 825 | 0 |
| HO458123 | 395 | 430 | 824 | 0 |
| HO458123 | 288 | 143 | 429 | 0 |
| HO777638 | 45 | 150 | 194 | 8.6 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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