| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os02g44510.1 |
| Family | GT24 |
| Protein Properties | Length: 653 Molecular Weight: 74999 Isoelectric Point: 6.7386 |
| Chromosome | Chromosome/Scaffold: 2 Start: 26943091 End: 26951443 |
| Description | UDP-glucose:glycoprotein glucosyltransferases;transferases, transferring hexosyl groups;transferases, transferring glycosyl groups |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT24 | 351 | 598 | 0 |
| INIFSVASGHLYERFLKIMILSVLKQTQRPVKFWFIKNYLSPQFKDVIPHMAQEYGFEYELVTYKWPTWLHKQKEKQRIIWAYKILFLDVIFPLSLRKVI FVDADQIVRADMGELYDMNLKGRPLAYTPFCDNNKEMDGYRFWKQGFWKDHLRGRPYHISALYVVDLAKFRQTASGDTLRVFYETLSKDPNSLSNLDQDL PNYAQHTVPIFSLPQEWLWCESWCGNATKARAKTIDLCNNPMTKEPKL | |||
| Full Sequence |
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| Protein Sequence Length: 653 Download |
| MEYELRTKHI YEIIEEIEWT GVDPDDLTSK FYSDVAMLIS SSMSIRERPS ERAHFEILHA 60 EHSAIKLNGM SSGVHIDAVI DPLSPAGQKL APLLRILWRQ IQPSMRIVLN PISSLADLPL 120 KNYYRFVLPS MDDFSSTDYS VHGPKAFFAN MPLSKTLTMN IDVPEPWLVE PVIAIHDLDN 180 ILLENLGDVR TLQAVFELEA LLLTGHCKEK DRDPPRGLQF ILGTKRRPHL VDTLVMANLG 240 YWQMKVSPGV WYLQLAPGRS ADLYELPSKL IAIDSLRGKL MHIEVQKRGG KEHEDLLNAD 300 DDNHFQEKMD NKGWNNNLLK WASSFISGDS SSKKKDEKIS DLKATRQGET INIFSVASGH 360 LYERFLKIMI LSVLKQTQRP VKFWFIKNYL SPQFKDVIPH MAQEYGFEYE LVTYKWPTWL 420 HKQKEKQRII WAYKILFLDV IFPLSLRKVI FVDADQIVRA DMGELYDMNL KGRPLAYTPF 480 CDNNKEMDGY RFWKQGFWKD HLRGRPYHIS ALYVVDLAKF RQTASGDTLR VFYETLSKDP 540 NSLSNLDQDL PNYAQHTVPI FSLPQEWLWC ESWCGNATKA RAKTIDLCNN PMTKEPKLQG 600 AKRIVPEWVD LDSEARQFTA RILGDNPESP GTTSPPSDTP KSDDKGAKHD EL* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam01501 | Glyco_transf_8 | 0.0005 | 355 | 550 | 203 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd04194 | GT8_A4GalT_like | 4.0e-13 | 351 | 567 | 226 | + A4GalT_like proteins catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The members of this family of glycosyltransferases catalyze the addition of galactose or glucose residues to the lipooligosaccharide (LOS) or lipopolysaccharide (LPS) of the bacterial cell surface. The enzymes exhibit broad substrate specificities. The known functions found in this family include: Alpha-1,4-galactosyltransferase, LOS-alpha-1,3-D-galactosyltransferase, UDP-glucose:(galactosyl) LPS alpha1,2-glucosyltransferase, UDP-galactose: (glucosyl) LPS alpha1,2-galactosyltransferase, and UDP-glucose:(glucosyl) LPS alpha1,2-glucosyltransferase. Alpha-1,4-galactosyltransferase from N. meningitidis adds an alpha-galactose from UDP-Gal (the donor) to a terminal lactose (the acceptor) of the LOS structure of outer membrane. LOSs are virulence factors that enable the organism to evade the immune system of host cells. In E. coli, the three alpha-1,2-glycosyltransferases, that are involved in the synthesis of the outer core region of the LPS, are all members of this family. The three enzymes share 40 % of sequence identity, but have different sugar donor or acceptor specificities, representing the structural diversity of LPS. | ||
| cd00505 | Glyco_transf_8 | 9.0e-56 | 351 | 598 | 257 | + Members of glycosyltransferase family 8 (GT-8) are involved in lipopolysaccharide biosynthesis and glycogen synthesis. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. GT-8 comprises enzymes with a number of known activities: lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, glycogenin glucosyltransferase, and N-acetylglucosaminyltransferase. GT-8 enzymes contains a conserved DXD motif which is essential in the coordination of a catalytic divalent cation, most commonly Mn2+. | ||
| pfam06427 | UDP-g_GGTase | 1.0e-89 | 33 | 238 | 212 | + UDP-glucose:Glycoprotein Glucosyltransferase. The N-terminal region of this group of proteins is required for correct folding of the ER UDP-Glc: glucosyltransferase. | ||
| cd06432 | GT8_HUGT1_C_like | 3.0e-179 | 351 | 598 | 248 | + The C-terminal domain of HUGT1-like is highly homologous to the GT 8 family. C-terminal domain of glycoprotein glucosyltransferase (UGT). UGT is a large glycoprotein whose C-terminus contains the catalytic activity. This catalytic C-terminal domain is highly homologous to Glycosyltransferase Family 8 (GT 8) and contains the DXD motif that coordinates donor sugar binding, characteristic for Family 8 glycosyltransferases. GT 8 proteins are retaining enzymes based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. The non-catalytic N-terminal portion of the human UTG1 (HUGT1) has been shown to monitor the protein folding status and activate its glucosyltransferase activity. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003980 | UDP-glucose:glycoprotein glucosyltransferase activity |
| GO:0005783 | endoplasmic reticulum |
| GO:0005975 | carbohydrate metabolic process |
| GO:0006464 | cellular protein modification process |
| GO:0006486 | protein glycosylation |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD28382.1 | 0 | 1 | 652 | 950 | 1626 | putative UDP-glucose:glycoprotein glucosyltransferase [Oryza sativa Japonica Group] |
| GenBank | EEE57527.1 | 0 | 1 | 652 | 921 | 1597 | hypothetical protein OsJ_07839 [Oryza sativa Japonica Group] |
| RefSeq | NP_177278.3 | 0 | 1 | 640 | 940 | 1591 | EBS1 (EMS-mutagenized bri1 suppressor 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002268972.1 | 0 | 1 | 651 | 941 | 1605 | PREDICTED: similar to EBS1 (EMS-MUTAGENIZED BRI1 SUPPRESSOR 1); UDP-glucose:glycoprotein glucosyltransferase/ transferase, transferring glycosyl groups / transferase, transferring hexosyl groups [Vitis vinifera] |
| RefSeq | XP_002454743.1 | 0 | 1 | 645 | 914 | 1558 | hypothetical protein SORBIDRAFT_04g036540 [Sorghum bicolor] |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| HO415354 | 543 | 104 | 622 | 0 |
| GO883503 | 317 | 312 | 628 | 0 |
| HO797114 | 337 | 292 | 628 | 0 |
| DR944618 | 276 | 346 | 621 | 0 |
| HO415354 | 110 | 1 | 105 | 0.000000005 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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