| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os02g54820.3 |
| Family | GT20 |
| Protein Properties | Length: 751 Molecular Weight: 83427.4 Isoelectric Point: 7.2742 |
| Chromosome | Chromosome/Scaffold: 2 Start: 33569263 End: 33573174 |
| Description | trehalose-phosphatase/synthase 9 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 49 | 532 | 0 |
| ARRVVVSHRLPLRAAADAASPFGFSFTVDSDAVAYQLRSGLPPGAPVLHIGTLPPPATEAASDELCNYLLANFSCLPVYLPADLHRRFYHGFCKHYLWPL LHYLLPLTPSSLGGLPFDRALYHSFLSANRAFADRLTEVLSPDDDLVWIHDYHLLALPTFLRKRFPRAKVGFFLHSPFPSSEIFRTIPVREDLLRALLNA DLVGFHTFDYARHFLSACSRLLGLDYQSKRGYIGIEYYGRTVTVKILPVGIDMGQLRSVVSAPETGDLVRRLTESYKGRRLMVGVDDVDLFKGIGLKFLA MEQLLVEHPELRGRAVLVQIANPARSEGRDIQEVQGEARAISARVNARFGTPGYTPIVLIDRGVSVHEKAAYYAAAECCVVSAVRDGLNRIPYIYTVCRQ ESTGLDDAAKRSVIVLSEFVGCSPSLSGAIRVNPWSVESMAEAMNAALRMPEPEQRLRHEKHYKYVSTHDVAYWAKSFDQDLQR | |||
| Full Sequence |
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| Protein Sequence Length: 751 Download |
| MPSLSCHNLL DLVAAADDAA PSPASLRLPR VMSAASPASP TSPSTPAPAR RVVVSHRLPL 60 RAAADAASPF GFSFTVDSDA VAYQLRSGLP PGAPVLHIGT LPPPATEAAS DELCNYLLAN 120 FSCLPVYLPA DLHRRFYHGF CKHYLWPLLH YLLPLTPSSL GGLPFDRALY HSFLSANRAF 180 ADRLTEVLSP DDDLVWIHDY HLLALPTFLR KRFPRAKVGF FLHSPFPSSE IFRTIPVRED 240 LLRALLNADL VGFHTFDYAR HFLSACSRLL GLDYQSKRGY IGIEYYGRTV TVKILPVGID 300 MGQLRSVVSA PETGDLVRRL TESYKGRRLM VGVDDVDLFK GIGLKFLAME QLLVEHPELR 360 GRAVLVQIAN PARSEGRDIQ EVQGEARAIS ARVNARFGTP GYTPIVLIDR GVSVHEKAAY 420 YAAAECCVVS AVRDGLNRIP YIYTVCRQES TGLDDAAKRS VIVLSEFVGC SPSLSGAIRV 480 NPWSVESMAE AMNAALRMPE PEQRLRHEKH YKYVSTHDVA YWAKSFDQDL QRACKDHFSR 540 RHWGIGFGMS FKVVALGPNF RRLSVDHIVP SYRKSDNRLI LLDYDGTVMP EGSIDKAPSN 600 EVISVLNRLC EDPKNRVFIV SGRGKDELGR WFAPCEKLGI AAEHGYFTRW SRDSAWETCG 660 LAVDFDWKKT AEPVMRLYKE ATDGSTIEDK ESALVWHHDE ADPDFGSCQA KELLDHLENV 720 LANEPVVVKR GQHIVEVNPQ VSACHLPSFL * 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 6.0e-136 | 118 | 739 | 629 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PRK14501 | PRK14501 | 4.0e-161 | 50 | 740 | 700 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| PLN02205 | PLN02205 | 0 | 50 | 740 | 701 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| cd03788 | GT1_TPS | 0 | 50 | 531 | 482 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| pfam00982 | Glyco_transf_20 | 0 | 50 | 532 | 486 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005739 | mitochondrion |
| GO:0005829 | cytosol |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD19111.1 | 0 | 1 | 750 | 1 | 750 | putative alpha,alpha-trehalose-phosphate synthase [Oryza sativa Japonica Group] |
| GenBank | EAY87814.1 | 0 | 1 | 740 | 1 | 740 | hypothetical protein OsI_09233 [Oryza sativa Indica Group] |
| RefSeq | NP_001048355.1 | 0 | 243 | 750 | 1 | 508 | Os02g0790500 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001148318.1 | 0 | 1 | 740 | 1 | 741 | LOC100281927 [Zea mays] |
| RefSeq | XP_002452959.1 | 0 | 1 | 740 | 1 | 741 | hypothetical protein SORBIDRAFT_04g035560 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 136 | 532 | 76 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 136 | 532 | 76 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 136 | 532 | 76 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 136 | 532 | 76 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 136 | 532 | 75 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 680 | 72 | 740 | 0 |
| HO779327 | 512 | 95 | 599 | 0 |
| HO779327 | 146 | 595 | 740 | 0 |
| CB653065 | 277 | 212 | 488 | 0 |
| CB635432 | 272 | 176 | 447 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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