| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os02g57770.1 |
| Family | GH16 |
| Protein Properties | Length: 328 Molecular Weight: 36339.8 Isoelectric Point: 9.3639 |
| Chromosome | Chromosome/Scaffold: 2 Start: 35376562 End: 35378827 |
| Description | xyloglucan endotransglucosylase/hydrolase 32 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 71 | 229 | 1.1e-21 |
| ETGVTVWLDRRSGSGFKSRRAYRSGYFGAWVRLQRGYTAGVITAFYLSNGEAHPGWHDEVDMEFLGTTPGKPYTLQTNVFSLGSGDPPRSLGREIKFHLW FDPTADFHHYAILWTSDHIIFLVDDVPIRRYGRRSAGGAAGFPARPMWVYGSIWDASSW | |||
| Full Sequence |
|---|
| Protein Sequence Length: 328 Download |
| MAIIGRRQQQ GVAAAAATLV ALMAVVVAAA AEAQPSPGVY PSRMFRAREF GRDFRSLWGA 60 EHQQQEAAAP ETGVTVWLDR RSGSGFKSRR AYRSGYFGAW VRLQRGYTAG VITAFYLSNG 120 EAHPGWHDEV DMEFLGTTPG KPYTLQTNVF SLGSGDPPRS LGREIKFHLW FDPTADFHHY 180 AILWTSDHII FLVDDVPIRR YGRRSAGGAA GFPARPMWVY GSIWDASSWA TEDGRYRADY 240 SYQPFVARFS AFLLRGCSPH APRTCAAPVA GDLTAAQLAA MRWAQRFHMV YNYCYDPKRD 300 HSLTPECRTH LHPSSSSSNS SSSSYHG* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 2.0e-16 | 95 | 247 | 162 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| cd00413 | Glyco_hydrolase_16 | 3.0e-20 | 79 | 243 | 166 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| PLN03161 | PLN03161 | 1.0e-53 | 78 | 307 | 244 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| pfam00722 | Glyco_hydro_16 | 1.0e-57 | 50 | 242 | 193 | + Glycosyl hydrolases family 16. | ||
| cd02176 | GH16_XET | 2.0e-107 | 50 | 307 | 268 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005576 | extracellular region |
| GO:0005618 | cell wall |
| GO:0005623 | cell |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABU93485.1 | 0 | 22 | 307 | 5 | 291 | xyloglucan endotraglucosylase/hydrolase [Vigna angularis] |
| GenBank | ACU17768.1 | 0 | 19 | 307 | 6 | 291 | unknown [Glycine max] |
| DDBJ | BAD22925.1 | 0 | 1 | 327 | 1 | 327 | putative xyloglucan endo-transglycosylase [Oryza sativa Japonica Group] |
| GenBank | EAZ25136.1 | 0 | 34 | 300 | 12 | 278 | hypothetical protein OsJ_08934 [Oryza sativa Japonica Group] |
| RefSeq | NP_001141552.1 | 0 | 19 | 310 | 20 | 317 | hypothetical protein LOC100273667 [Zea mays] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2uwc_B | 0 | 32 | 307 | 1 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| PDB | 2uwc_A | 0 | 32 | 307 | 1 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
| PDB | 2uwa_C | 0 | 29 | 307 | 1 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| PDB | 2uwa_B | 0 | 29 | 307 | 1 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
| PDB | 2uwa_A | 0 | 29 | 307 | 1 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |
