| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os03g12360.2 |
| Family | GT20 |
| Protein Properties | Length: 861 Molecular Weight: 96634.7 Isoelectric Point: 6.0456 |
| Chromosome | Chromosome/Scaffold: 3 Start: 6519051 End: 6523487 |
| Description | UDP-Glycosyltransferase / trehalose-phosphatase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 59 | 550 | 0 |
| PRTIVVANHLPIRAHRPASPSEPWTFSWDEDSLLRHLQHSSSSPAMEFIYIGCLRDDIPLADQDAVAQALLESYNCVPAFLPPDIAERYYHGFCKQHLWP LFHYMLPLSPDLGGRFDRALWQSYVSANKIFADKVLEVINPDDDFVWVHDYHLMVLPTFLRKRFNRIKLGFFLHSPFPSSEIYKTLPVREELLRALLNSD LIGFHTFDYARHFLSCCGRMLGLSYESKRGHICLEYYGRTVSIKILPVGVNMGQLKTVLALPETEAKVAELMATYSGKGRVVMLGVDDMDIFKGISLKLL AMEELLRQHPEWRGKLVLVQVANPARGRGKDVDEVKGETYAMVRRINEAYGAPGYEPVVLIDEPLQFYERVAYYVVAEVCLVTAVRDGMNLIPYEYIVSR QGNEALDRMLQPSKPEEKKSMLVVSEFIGCSPSLSGAVRVNPWNIEAVADAMESALVLPEKEKRMRHDKHYRYVDTHDVGYWATSFLQDLER | |||
| Full Sequence |
|---|
| Protein Sequence Length: 861 Download |
| MVSRSYSNLL DLATGAADQA PAPAALGALR RRLPRVVTTA GLIDDSPLSP STPSPSPRPR 60 TIVVANHLPI RAHRPASPSE PWTFSWDEDS LLRHLQHSSS SPAMEFIYIG CLRDDIPLAD 120 QDAVAQALLE SYNCVPAFLP PDIAERYYHG FCKQHLWPLF HYMLPLSPDL GGRFDRALWQ 180 SYVSANKIFA DKVLEVINPD DDFVWVHDYH LMVLPTFLRK RFNRIKLGFF LHSPFPSSEI 240 YKTLPVREEL LRALLNSDLI GFHTFDYARH FLSCCGRMLG LSYESKRGHI CLEYYGRTVS 300 IKILPVGVNM GQLKTVLALP ETEAKVAELM ATYSGKGRVV MLGVDDMDIF KGISLKLLAM 360 EELLRQHPEW RGKLVLVQVA NPARGRGKDV DEVKGETYAM VRRINEAYGA PGYEPVVLID 420 EPLQFYERVA YYVVAEVCLV TAVRDGMNLI PYEYIVSRQG NEALDRMLQP SKPEEKKSML 480 VVSEFIGCSP SLSGAVRVNP WNIEAVADAM ESALVLPEKE KRMRHDKHYR YVDTHDVGYW 540 ATSFLQDLER TCKDHAQRRC WGIGFGLRFR VVSLDLSFRK LAMEHIVMAY RRAKTRAILL 600 DYDGTLMPQA INKSPSANSV ETLTSLCRDK SNKVFLCSGF EKGTLHDWFP CENLGLAAEH 660 GYFLRSSRDA EWEISIPPAD CSWKQIAEPV MCLYRETTDG SIIENRETVL VWNYEDADPD 720 FGSCQAKELV DHLESVLANE PVSVKSTGHS VEVKPQGVSK GLVARRLLAS MQERGMCTDF 780 VLCIGDDRSD EEMFQMITSS TCGESLAATA EVFACTVGRK PSKAKYYLDD TAEVVRLMQG 840 LASVSNELAR AASPPEDDDE * 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 1.0e-159 | 63 | 849 | 825 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN02205 | PLN02205 | 0 | 63 | 847 | 787 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 63 | 550 | 490 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 130 | 844 | 718 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| cd03788 | GT1_TPS | 0 | 63 | 549 | 487 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005992 | trehalose biosynthetic process |
| GO:0008152 | metabolic process |
| GO:0009058 | biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAN52740.1 | 0 | 1 | 860 | 1 | 860 | Putative trehalose-6-phosphate synthase [Oryza sativa Japonica Group] |
| GenBank | ADA67991.1 | 0 | 1 | 851 | 1 | 847 | trehalose-6-phosphate synthase [Zea mays] |
| GenBank | EEC74792.1 | 0 | 1 | 860 | 1 | 860 | hypothetical protein OsI_10580 [Oryza sativa Indica Group] |
| RefSeq | NP_001049428.1 | 0 | 105 | 860 | 1 | 756 | Os03g0224300 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002465620.1 | 0 | 1 | 859 | 1 | 855 | hypothetical protein SORBIDRAFT_01g042380 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 145 | 559 | 74 | 462 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 145 | 559 | 74 | 462 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 145 | 559 | 74 | 462 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 145 | 559 | 74 | 462 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 145 | 559 | 74 | 462 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 775 | 82 | 853 | 0 |
| HO779327 | 513 | 105 | 616 | 0 |
| HO779327 | 240 | 612 | 850 | 0 |
| HO797286 | 479 | 380 | 855 | 0 |
| HO795560 | 419 | 438 | 853 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
