| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os03g52560.1 |
| Family | GH32 |
| Protein Properties | Length: 366 Molecular Weight: 39730.6 Isoelectric Point: 6.0551 |
| Chromosome | Chromosome/Scaffold: 3 Start: 30149347 End: 30151269 |
| Description | cell wall invertase 2 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 37 | 142 | 7.3e-21 |
| VKHVLEVSLDLKRYEYYTFGEYDHAADAYVPDAALADGDDGLRYDYGNFYASKTFLDTARHRRVLWGWANESDSAADDVRKGWAGVQAIPRKVWLAPDGK QLMQWP | |||
| Full Sequence |
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| Protein Sequence Length: 366 Download |
| MAVIFQLDIR HPFYYCRNLV STVVTSHGVG GGGGVKVKHV LEVSLDLKRY EYYTFGEYDH 60 AADAYVPDAA LADGDDGLRY DYGNFYASKT FLDTARHRRV LWGWANESDS AADDVRKGWA 120 GVQAIPRKVW LAPDGKQLMQ WPVAEIESLR GNHVNVTDKL VRGGGGGNYF EVSGLATPAQ 180 ADVEATFQVM DLDKAEPFDP AWRGADAQAV CAARGADARG GVGPFGLWVL ASDELKERTA 240 VFFSVFKRDD ADVARVDGGK KHVVLTCNDP SRSSYAEQLY KPTYAGFVDV DIAPTGKISL 300 RTLIDHSVVE SFGGHGKTAI LARVYPTKAV GDKARLFVFN NGESDVKVTN LNAYDMGSAK 360 ITTDA* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG1621 | SacC | 2.0e-7 | 85 | 163 | 81 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| pfam08244 | Glyco_hydro_32C | 1.0e-12 | 224 | 316 | 94 | + Glycosyl hydrolases family 32 C terminal. This domain corresponds to the C terminal domain of glycosyl hydrolase family 32. It forms a beta sandwich module. | ||
| cd08996 | GH32_B_Fructosidase | 8.0e-14 | 85 | 145 | 61 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 6.0e-22 | 85 | 142 | 60 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 8.0e-49 | 41 | 316 | 279 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAP03410.1 | 0 | 1 | 365 | 1 | 365 | putative cell wall invertase [Oryza sativa Japonica Group] |
| EMBL | CAD40590.2 | 0 | 36 | 364 | 268 | 582 | OJ000126_13.7 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001051188.1 | 0 | 20 | 365 | 15 | 360 | Os03g0735800 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001052747.1 | 0 | 36 | 364 | 271 | 585 | Os04g0413200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002466502.1 | 0 | 38 | 364 | 258 | 577 | hypothetical protein SORBIDRAFT_01g008910 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qqv_A | 0 | 37 | 362 | 226 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (E203a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2oxb_A | 0 | 37 | 362 | 226 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (E203q) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2qqw_A | 0 | 37 | 362 | 226 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 37 | 362 | 226 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 37 | 362 | 226 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| DV857772 | 316 | 41 | 355 | 0 |
| CT842376 | 316 | 41 | 356 | 0 |
| FL871618 | 254 | 103 | 356 | 0 |
| EX268433 | 273 | 84 | 356 | 0 |
| FF355752 | 244 | 84 | 327 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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