| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os04g45290.1 |
| Family | GH32 |
| Protein Properties | Length: 476 Molecular Weight: 51973.5 Isoelectric Point: 4.4644 |
| Chromosome | Chromosome/Scaffold: 4 Start: 26765199 End: 26771893 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 2 | 267 | 0 |
| VPDHWYDINGVWTGSATQLPDGRIVMLYTGATEESVQVQNLAEPADPNDPLLREWSKAEANPVLVPPPGIGLTDFRDPTTAWRNPADSAWRITIGSKDRD HAGLALVYKTEDFLHYDLLPTLLHVVKGTGMWECVDLYPVSTSPAVEDGLETSTPPGPGVKHVLKASLDDDRNDYYAIGTYDGETDTWTPDNADIDVGIG LRYDYGKFYASKTFYDPVGRRRVLWGWIGETDSERADILKGWASLQSIPRTVMLDTKTGSNLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 476 Download |
| MVPDHWYDIN GVWTGSATQL PDGRIVMLYT GATEESVQVQ NLAEPADPND PLLREWSKAE 60 ANPVLVPPPG IGLTDFRDPT TAWRNPADSA WRITIGSKDR DHAGLALVYK TEDFLHYDLL 120 PTLLHVVKGT GMWECVDLYP VSTSPAVEDG LETSTPPGPG VKHVLKASLD DDRNDYYAIG 180 TYDGETDTWT PDNADIDVGI GLRYDYGKFY ASKTFYDPVG RRRVLWGWIG ETDSERADIL 240 KGWASLQSIP RTVMLDTKTG SNLLQWPVVE VENLRMRGKS FDGLDVSPGS VVPLDVGKAT 300 QLDIEAVFEV DTSAADGVVT EAGAAAYSCG TGGGAVGRGL MGPFGLLVLA DDQLSERTAV 360 FFYLVKGVDG NLTTFFCQDE LRSSKANDLV KRVYGSLVPV LDGENLSIRI LVDHSIVEGF 420 AQGGRTCITS RVYPTKAIYE SAKIFLFNNA TNVRVTAKSL KIWELNSAYI RPYVD* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 2.0e-29 | 3 | 434 | 449 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-36 | 3 | 435 | 444 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 7.0e-58 | 1 | 270 | 281 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 3.0e-108 | 1 | 267 | 274 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 9.0e-146 | 1 | 424 | 433 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005773 | vacuole |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAF87245.1 | 0 | 1 | 475 | 172 | 652 | AF276703_1 vacuolar acid invertase [Oryza sativa] |
| EMBL | CAD41525.3 | 0 | 1 | 475 | 175 | 666 | OSJNBb0020O11.6 [Oryza sativa (japonica cultivar-group)] |
| EMBL | CAH67112.1 | 0 | 1 | 475 | 175 | 649 | H0502G05.3 [Oryza sativa (indica cultivar-group)] |
| GenBank | EEC77707.1 | 0 | 1 | 475 | 182 | 673 | hypothetical protein OsI_16783 [Oryza sativa Indica Group] |
| RefSeq | NP_001053422.1 | 0 | 1 | 475 | 182 | 656 | Os04g0535600 [Oryza sativa (japonica cultivar-group)] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 1 | 473 | 80 | 544 | A Chain A, Crystal Structure Of Rice Bglu1 E386s Mutant |
| PDB | 3ugh_A | 0 | 1 | 473 | 80 | 544 | A Chain A, Crystal Structure Of Rice Bglu1 E386s Mutant |
| PDB | 3ugg_B | 0 | 1 | 473 | 80 | 544 | A Chain A, Crystal Structure Of Rice Bglu1 E386s Mutant |
| PDB | 3ugg_A | 0 | 1 | 473 | 80 | 544 | A Chain A, Crystal Structure Of Rice Bglu1 E386s Mutant |
| PDB | 3ugf_B | 0 | 1 | 473 | 80 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CB651234 | 262 | 215 | 476 | 0 |
| CB675354 | 231 | 1 | 231 | 0 |
| CB675355 | 221 | 256 | 476 | 0 |
| GW667497 | 317 | 153 | 469 | 0 |
| GO882338 | 347 | 91 | 430 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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