| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os04g56920.1 |
| Family | GH32 |
| Protein Properties | Length: 597 Molecular Weight: 64708.4 Isoelectric Point: 5.0747 |
| Chromosome | Chromosome/Scaffold: 4 Start: 33940929 End: 33943427 |
| Description | beta-fructofuranosidase 5 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 42 | 371 | 0 |
| HFQPAKNWQNGAYGPVYYNGMYHLFYQYNPHGALWDVGNLSWGHSVSGDLVNWAALDNALDPTAPFDANGCASGSVTILPDGVPVVMYSGIDARRRQVQN VAFPKNPRDPLLREWTKPGYNPVIPVPADVSPDNFRDPTTAWLGSDGLWRFAISAVADGVGATLVYRSADFLRWERNAAPLHASRDAVMAECPDLFPVAE HGEDGLDLDASAIGGAGAGVRHVLKVSMPDTLEDYYMVGRYDDADDTFTVPPEDLEAHGDDYRRWRRIDHGHLYASKTFYDAGKKRRVLWAWVNESDSEA DDVTKGWSGLQSFPRAVWLDEGGRQLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 597 Download |
| MALAGLPLSV FAIAVHFCLV FSSSSSPPVC PANGHRDRTA YHFQPAKNWQ NGAYGPVYYN 60 GMYHLFYQYN PHGALWDVGN LSWGHSVSGD LVNWAALDNA LDPTAPFDAN GCASGSVTIL 120 PDGVPVVMYS GIDARRRQVQ NVAFPKNPRD PLLREWTKPG YNPVIPVPAD VSPDNFRDPT 180 TAWLGSDGLW RFAISAVADG VGATLVYRSA DFLRWERNAA PLHASRDAVM AECPDLFPVA 240 EHGEDGLDLD ASAIGGAGAG VRHVLKVSMP DTLEDYYMVG RYDDADDTFT VPPEDLEAHG 300 DDYRRWRRID HGHLYASKTF YDAGKKRRVL WAWVNESDSE ADDVTKGWSG LQSFPRAVWL 360 DEGGRQLVQW PVEEIETLRR KRGVLLGGNE VEAGGLREIG GIAGSQADVE VAFEIASLAG 420 ADRLEPDHLR DPDALCGENG AAVHGGIGPF GLLVMASGDL RERTAVFFRV FRLSHGYTVL 480 MCTDLTRSTS RAGVYKPSHG GFVDIDIEKD RAISLRTLID HSIVESFGGG GRTCMTARVY 540 PEHVATGSSH LYVFNNASDA VKVSKLEAWE LATASVNAGD DGLISYGGPV CAAQVQ* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 3.0e-39 | 38 | 380 | 371 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 4.0e-57 | 37 | 549 | 525 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 8.0e-81 | 55 | 374 | 326 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 5.0e-128 | 42 | 371 | 338 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 7.0e-153 | 42 | 531 | 500 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005773 | vacuole |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE03580.1 | 0 | 1 | 596 | 1 | 593 | OSJNBa0087O24.3 [Oryza sativa (japonica cultivar-group)] |
| EMBL | CAH65975.1 | 0 | 1 | 596 | 1 | 593 | H1005F08.4 [Oryza sativa (indica cultivar-group)] |
| RefSeq | NP_001054172.1 | 0 | 1 | 589 | 1 | 586 | Os04g0664800 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | Q56UD0 | 0 | 1 | 596 | 1 | 596 | INV6_ORYSJ RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 6; AltName: Full=Sucrose hydrolase 6; AltName: Full=Invertase 6; AltName: Full=Cell wall beta-fructosidase 6; AltName: Full=OsCIN6; Flags: Precursor |
| RefSeq | XP_002448711.1 | 0 | 38 | 595 | 39 | 586 | hypothetical protein SORBIDRAFT_06g031910 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 38 | 574 | 8 | 531 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
| PDB | 2ade_A | 0 | 38 | 574 | 8 | 531 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
| PDB | 2add_A | 0 | 38 | 574 | 8 | 531 | A Chain A, Characterization And Engineering Of The Bifunctional N- And O-glucosyltransferase Involved In Xenobiotic Metabolism In Plants |
| PDB | 1st8_A | 0 | 38 | 574 | 8 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 38 | 574 | 8 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
