| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os04g56930.1 |
| Family | GH32 |
| Protein Properties | Length: 506 Molecular Weight: 55165 Isoelectric Point: 5.5513 |
| Chromosome | Chromosome/Scaffold: 4 Start: 33943991 End: 33946520 |
| Description | beta-fructofuranosidase 5 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 18 | 288 | 0 |
| AGPLYHNGLYHFFYQYNPHGPLWDTGKLSWGHSVSGDLVNWAFLGTAIDPTDPFDVNGCWSGSATVLLGGRPAFLYTGRDAGGVQVQNVSFAKNPLDPLL REWEKPSCNPIIAFPADVINNNFRDPTTAWLGRDGLWRMVVAAEVAGAGSALVYRSADFLRWERNAAPMHSSAAVPVLECPDFFPVAEHGIDGLDTSANG GGTGVKHVLKLSEFDTHQDFYMARRNRRVQWLWVNEYDSKADDVAKGWAGVQAFPRKVWLDGDGKQLLQWP | |||
| Full Sequence |
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| Protein Sequence Length: 506 Download |
| MAELPTTSSL PSSGRMMAGP LYHNGLYHFF YQYNPHGPLW DTGKLSWGHS VSGDLVNWAF 60 LGTAIDPTDP FDVNGCWSGS ATVLLGGRPA FLYTGRDAGG VQVQNVSFAK NPLDPLLREW 120 EKPSCNPIIA FPADVINNNF RDPTTAWLGR DGLWRMVVAA EVAGAGSALV YRSADFLRWE 180 RNAAPMHSSA AVPVLECPDF FPVAEHGIDG LDTSANGGGT GVKHVLKLSE FDTHQDFYMA 240 RRNRRVQWLW VNEYDSKADD VAKGWAGVQA FPRKVWLDGD GKQLLQWPVD EIETLRTKRV 300 GLQGTEVKAG GLHEIVGVAS SQADVEVVFE IPNLEDEAES FDPDWLDPHK LCKDKGAASA 360 HGGVGPFGLI VMASGDLQEQ TAVFFRVFKH HGKYKVFMCT DLTRSSTKAD VYKDAYGGFV 420 DVDIQKDKSI SLRTLIDHSM IESFGGGGRA CITTRVYPEH AATSSSHLYV FNNGSGTVNV 480 SKLEAWEMAT ATVNSADALD AITRS* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 8.0e-29 | 19 | 458 | 478 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-38 | 11 | 482 | 509 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 6.0e-67 | 19 | 291 | 301 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-83 | 19 | 288 | 310 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 7.0e-115 | 19 | 448 | 477 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005618 | cell wall |
| GO:0006950 | response to stress |
| GO:0008152 | metabolic process |
| GO:0009628 | response to abiotic stimulus |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAT84405.1 | 0 | 19 | 505 | 31 | 517 | cell-wall invertase 5 [Oryza sativa Japonica Group] |
| EMBL | CAE03581.1 | 0 | 19 | 505 | 28 | 514 | OSJNBa0087O24.4 [Oryza sativa (japonica cultivar-group)] |
| EMBL | CAH65976.1 | 0 | 19 | 505 | 28 | 555 | H1005F08.5 [Oryza sativa (indica cultivar-group)] |
| RefSeq | NP_001054173.1 | 0 | 17 | 505 | 1 | 530 | Os04g0664900 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | Q56UD1 | 0 | 19 | 505 | 56 | 542 | INV5_ORYSJ RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 5; AltName: Full=Sucrose hydrolase 5; AltName: Full=Invertase 5; AltName: Full=Cell wall beta-fructosidase 5; AltName: Full=OsCIN5 |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2aey_A | 0 | 19 | 491 | 25 | 531 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
| PDB | 2ade_A | 0 | 19 | 491 | 25 | 531 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
| PDB | 2add_A | 0 | 19 | 491 | 25 | 531 | B Chain B, The Avrptob-Bak1 Complex Reveals Two Structurally Similar Kinaseinteracting Domains In A Single Type Iii Effector |
| PDB | 1st8_A | 0 | 19 | 491 | 25 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
| PDB | 2aez_A | 0 | 19 | 491 | 25 | 531 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EE591202 | 424 | 123 | 506 | 0 |
| EE591202 | 190 | 19 | 204 | 0 |
| CT842376 | 520 | 19 | 496 | 0 |
| HO170744 | 249 | 242 | 489 | 0 |
| CT859868 | 203 | 19 | 220 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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