| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os05g27960.1 |
| Family | GH85 |
| Protein Properties | Length: 711 Molecular Weight: 79118 Isoelectric Point: 5.4938 |
| Chromosome | Chromosome/Scaffold: 5 Start: 16312800 End: 16319405 |
| Description | Glycosyl hydrolase family 85 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH85 | 101 | 381 | 0 |
| RGGYRDDAAPQGGHDPGAYALWHWHLIDVFVYFSHYLVTLPPPCWVNAAHLHGVKVLGTFITEWEKGAEICEEMLATEASAQMYAERLTELAAYLGFDGW LINIEVKLDIQFIDNLKEFINHLTKTMHAAVPGSLVIWYDAITIKGALDWQNKLNEYNKPFFDLCDGLFSNYTWKAKYPQESAVVAGERKYDVYMGIDVY GRNTFGGGQWNTNVALDLLKKDDVSAAIFAPGWVYETKQPPNFRTAQNRWWGLVQESWGVLQSYPKQLPFYSDFDQGHGYQ | |||
| Full Sequence |
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| Protein Sequence Length: 711 Download |
| MPFSPRRLLR RLRSMLPSAA PAAAAGEEEE EGERRPWEPP FDASQPAPPI SYPITTLAAL 60 ASRAYLSEAG NFHLPFNRAS SSPRAAPLPP RRRILACHDF RGGYRDDAAP QGGHDPGAYA 120 LWHWHLIDVF VYFSHYLVTL PPPCWVNAAH LHGVKVLGTF ITEWEKGAEI CEEMLATEAS 180 AQMYAERLTE LAAYLGFDGW LINIEVKLDI QFIDNLKEFI NHLTKTMHAA VPGSLVIWYD 240 AITIKGALDW QNKLNEYNKP FFDLCDGLFS NYTWKAKYPQ ESAVVAGERK YDVYMGIDVY 300 GRNTFGGGQW NTNVALDLLK KDDVSAAIFA PGWVYETKQP PNFRTAQNRW WGLVQESWGV 360 LQSYPKQLPF YSDFDQGHGY QVSIEGVKVY GAPWDNISCQ SFQPMLKYAG DRGLQTVINF 420 EDEPYSGGNC VTVKGSLQQN EIFSEQLFNG GLSMEGESVY VFYSVKADER SGLGLSLDLS 480 SGNNESSSIL IADDTAAFTR KKQHRKYGSY VKADKAEPHT PVHQNWVVYK ATILPSAGFT 540 LTGINIVCTM KTTSGTDPET DGDGSSEAGA NRSLHYHASL GHVSIRNTEE TEFPPARSWV 600 TEGDYISWSN GSDESKLASL KISWELENKQ QAPFMKYNVY VEKLTADSNA KAPRIFLGVA 660 SVQVFYVSDL EVPSEVTALK FFIQPCGRDG SCQGLHECPK FHLVPVDSAM * 720 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02874 | GH18_CFLE_spore_hydrolase | 0.001 | 125 | 230 | 114 | + Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis. | ||
| cd00598 | GH18_chitinase-like | 8.0e-8 | 100 | 252 | 175 | + The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model. | ||
| COG4724 | COG4724 | 2.0e-33 | 107 | 455 | 388 | + Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism] | ||
| cd06547 | GH85_ENGase | 1.0e-125 | 95 | 404 | 330 | + Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model. | ||
| pfam03644 | Glyco_hydro_85 | 4.0e-131 | 100 | 384 | 304 | + Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005737 | cytoplasm |
| GO:0008150 | biological_process |
| GO:0008152 | metabolic process |
| GO:0009536 | plastid |
| GO:0016787 | hydrolase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAV43808.1 | 0 | 15 | 710 | 1 | 696 | unknown protein [Oryza sativa Japonica Group] |
| GenBank | EAY97626.1 | 0 | 1 | 660 | 1 | 660 | hypothetical protein OsI_19549 [Oryza sativa Indica Group] |
| GenBank | EEE63344.1 | 0 | 1 | 710 | 1 | 710 | hypothetical protein OsJ_18155 [Oryza sativa Japonica Group] |
| RefSeq | NP_001055263.1 | 0 | 10 | 710 | 1 | 701 | Os05g0346500 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002440911.1 | 0 | 15 | 708 | 1 | 684 | hypothetical protein SORBIDRAFT_09g016230 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3fhq_F | 1e-31 | 123 | 478 | 83 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fhq_D | 1e-31 | 123 | 478 | 83 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fhq_B | 1e-31 | 123 | 478 | 83 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fhq_A | 1e-31 | 123 | 478 | 83 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fha_D | 1e-31 | 123 | 478 | 83 | 465 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CB670350 | 243 | 37 | 279 | 0 |
| CB670351 | 220 | 492 | 711 | 0 |
| CB634700 | 220 | 492 | 711 | 0 |
| CB670311 | 234 | 37 | 270 | 0 |
| JG939664 | 286 | 231 | 515 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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