| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os05g44100.1 |
| Family | GT20 |
| Protein Properties | Length: 900 Molecular Weight: 100534 Isoelectric Point: 5.4346 |
| Chromosome | Chromosome/Scaffold: 5 Start: 25641595 End: 25646409 |
| Description | trehalose-phosphatase/synthase 7 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 70 | 566 | 0 |
| AACERLIVVANQLPVVARRRPGAAAGGWAFSWDDDSLLLRLRDGVPDEMEVLFIGTLRADVPACEQDEVSQSLIDGFGCAPVFLPAGLYDRFYQHFCKGY LWPLFHYMLPFASALPAAASGDGRFDRGAWEAYVLANKYFFEKVVEVINPEDDYVWVHDYHLMALPTFLRRRFNRLRIGFFLHSPFPSSEIYRSLPVREE ILRTLLNCDLIGFHTFDYARHFLSCCSRMLGIEYQSKRGYIGLDYFGRTVGIKIMPVGIHMGQLQSVLRLSEKEKKVAELRQQFEGKSVLLGVDDMDIFK GINLKLLAFENMLRTHPKWKGRAVLVQIANPARGKGKDLEAVQAEIRESCDRINKEFGQSGYSPVIFIDQSVPSAVRLAYYTVAECVVVTAVRDGMNLTP YEYIVCREGIPGSECAPEVSGPKKSMLVVSEFIGCSPSLSGAIRVNPWNIEATAEALNEAISMSEREKQLRHEKHYRYVSTHDVAYWSKSFVQDLER | |||
| Full Sequence |
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| Protein Sequence Length: 900 Download |
| MMSRSYTNLL DLAAGNFEAL GPAGGGRRRS FGAKRMTRVM TVPGTLSELD DEDDEPAATS 60 SVASDVPSSA ACERLIVVAN QLPVVARRRP GAAAGGWAFS WDDDSLLLRL RDGVPDEMEV 120 LFIGTLRADV PACEQDEVSQ SLIDGFGCAP VFLPAGLYDR FYQHFCKGYL WPLFHYMLPF 180 ASALPAAASG DGRFDRGAWE AYVLANKYFF EKVVEVINPE DDYVWVHDYH LMALPTFLRR 240 RFNRLRIGFF LHSPFPSSEI YRSLPVREEI LRTLLNCDLI GFHTFDYARH FLSCCSRMLG 300 IEYQSKRGYI GLDYFGRTVG IKIMPVGIHM GQLQSVLRLS EKEKKVAELR QQFEGKSVLL 360 GVDDMDIFKG INLKLLAFEN MLRTHPKWKG RAVLVQIANP ARGKGKDLEA VQAEIRESCD 420 RINKEFGQSG YSPVIFIDQS VPSAVRLAYY TVAECVVVTA VRDGMNLTPY EYIVCREGIP 480 GSECAPEVSG PKKSMLVVSE FIGCSPSLSG AIRVNPWNIE ATAEALNEAI SMSEREKQLR 540 HEKHYRYVST HDVAYWSKSF VQDLERACKD HFRKPCWGIG LGFGFRVVAL DPHFTKLNFD 600 SIIMSYERSK SRAIFLDYDG TLVPQASLNK NPSEELLRII NTLCADRNNT VFIVSGRSKD 660 DLSKKLISCP KLGIAAEHGY FLRWTRDEEW QTTAQTSDFG WMQMAKPVMD LYTESTDGST 720 IETKETALVW HHQDADQGFG SSQAKEMLDH LESVLANEPV SVKSGQFIVE VKPQGVTKGL 780 IAEKVLTSMK EKGQLADFVL CIGDDRSDED MFENIADVMK RSIVAPKTPL FACTVGQKPS 840 KARFYLDDTF EVVTMLSSLA DASEPDLMAD LEDDLATSVS SIEISDRVVS FSNLRTEGS* 900 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03063 | PLN03063 | 1.0e-164 | 74 | 863 | 826 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN02205 | PLN02205 | 0 | 1 | 864 | 869 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming] | ||
| pfam00982 | Glyco_transf_20 | 0 | 73 | 566 | 498 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| PRK14501 | PRK14501 | 0 | 134 | 862 | 734 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| cd03788 | GT1_TPS | 0 | 74 | 565 | 492 | + Trehalose-6-Phosphate Synthase (TPS) is a glycosyltransferase that catalyses the synthesis of alpha,alpha-1,1-trehalose-6-phosphate from glucose-6-phosphate using a UDP-glucose donor. It is a key enzyme in the trehalose synthesis pathway. Trehalose is a nonreducing disaccharide present in a wide variety of organisms and may serve as a source of energy and carbon. It is characterized most notably in insect, plant, and microbial cells. Its production is often associated with a variety of stress conditions, including desiccation, dehydration, heat, cold, and oxidation. This family represents the catalytic domain of the TPS. Some members of this domain family coexist with a C-terminal trehalose phosphatase domain. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005829 | cytosol |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005992 | trehalose biosynthetic process |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | EEC79533.1 | 0 | 1 | 899 | 1 | 899 | hypothetical protein OsI_20637 [Oryza sativa Indica Group] |
| GenBank | EEE64369.1 | 0 | 1 | 899 | 1 | 899 | hypothetical protein OsJ_19211 [Oryza sativa Japonica Group] |
| RefSeq | NP_001056050.1 | 0 | 1 | 899 | 1 | 899 | Os05g0517200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002440081.1 | 0 | 1 | 899 | 1 | 906 | hypothetical protein SORBIDRAFT_09g025660 [Sorghum bicolor] |
| RefSeq | XP_002458490.1 | 0 | 1 | 899 | 1 | 910 | hypothetical protein SORBIDRAFT_03g034640 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 159 | 566 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 159 | 566 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 159 | 566 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 159 | 566 | 74 | 453 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1gz5_D | 0 | 159 | 566 | 73 | 452 | A Chain A, Trehalose-6-Phosphate Synthase. Otsa |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO777775 | 777 | 97 | 864 | 0 |
| HO779327 | 519 | 117 | 633 | 0 |
| HO779327 | 241 | 629 | 865 | 0 |
| HO795511 | 469 | 396 | 864 | 0 |
| HO797286 | 472 | 398 | 864 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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