| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os05g44210.1 |
| Family | GT20 |
| Protein Properties | Length: 860 Molecular Weight: 95210.2 Isoelectric Point: 6.717 |
| Chromosome | Chromosome/Scaffold: 5 Start: 25713720 End: 25720299 |
| Description | trehalose-6-phosphate synthase |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT20 | 126 | 594 | 0 |
| MKQRLLVVANRLPVSANRRGEDQWSLEISAGGLVSALLGVKDVDAKWIGWAGVNVPDEVGQRALTRALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFH YLGLPQEDRLATTRNFESQFNAYKRANQMFADVVYQHYKEGDVIWCHDYHLMFLPKCLKDHDINMKVGWFLHTPFPSSEIYRTLPSRSELLRSVLCADLV GFHTYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSERFKRALELPAVKRHITELTQRFDGRKVMLGVDRLDMIKGIPQKILAFEKFLEE NHEWNDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVALVTSLRDGMNLVSYEYVACQGSKKGVLI LSEFAGAAQSLGAGAILVNPWNITEVADSIKHALTMSSDEREKRHRHNYAHVTTHTAQDWAETFVCELN | |||
| Full Sequence |
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| Protein Sequence Length: 860 Download |
| MPTPAPSASS SSSSCGGGGG GAGAASSYSS SPDDRMLRGE CGRRHPFASS AAVGAGSPDA 60 MDTDSAEPSS AATSVADFGA RSPFSPGAAS PANMDDAGGA SAAGHAARPP LAGPRSGFRR 120 LGLRGMKQRL LVVANRLPVS ANRRGEDQWS LEISAGGLVS ALLGVKDVDA KWIGWAGVNV 180 PDEVGQRALT RALAEKRCIP VFLDEEIVHQ YYNGYCNNIL WPLFHYLGLP QEDRLATTRN 240 FESQFNAYKR ANQMFADVVY QHYKEGDVIW CHDYHLMFLP KCLKDHDINM KVGWFLHTPF 300 PSSEIYRTLP SRSELLRSVL CADLVGFHTY DYARHFVSAC TRILGLEGTP EGVEDQGRLT 360 RVAAFPIGID SERFKRALEL PAVKRHITEL TQRFDGRKVM LGVDRLDMIK GIPQKILAFE 420 KFLEENHEWN DKVVLLQIAV PTRTDVPEYQ KLTSQVHEIV GRINGRFGTL TAVPIHHLDR 480 SLDFHALCAL YAVTDVALVT SLRDGMNLVS YEYVACQGSK KGVLILSEFA GAAQSLGAGA 540 ILVNPWNITE VADSIKHALT MSSDEREKRH RHNYAHVTTH TAQDWAETFV CELNETVAEA 600 QLRTRQVPPD LPSQAAIQQY LHSKNRLLIL GFNSTLTEPV ESSGRRGGDQ IKEMELKLHP 660 ELKGPLRALC EDEHTTVIVL SGSDRSVLDE NFGEFNMWLA AEHGMFLRPT NGEWMTTMPE 720 HLNMDWVDSV KNVFEYFTER TPRSHFEHRE TSFVWNYKYA DVEFGRLQAR DMLQHLWTGP 780 ISNAAVDVVQ GSRSVEVRSV GVTKGAAIDR ILGEIVHSKS MITPIDYVLC IGHFLGKVIM 840 QLIYMCISVS FSARCFFCL* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN03064 | PLN03064 | 0 | 34 | 837 | 804 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| PLN03063 | PLN03063 | 0 | 124 | 837 | 714 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
| pfam00982 | Glyco_transf_20 | 0 | 128 | 595 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
| TIGR02400 | trehalose_OtsA | 0 | 129 | 595 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
| PRK14501 | PRK14501 | 0 | 129 | 832 | 709 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0000003 | reproduction |
| GO:0003824 | catalytic activity |
| GO:0005737 | cytoplasm |
| GO:0005975 | carbohydrate metabolic process |
| GO:0005992 | trehalose biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACI16353.1 | 0 | 126 | 837 | 1 | 712 | trehalose-6-P synthase [Triticum aestivum] |
| GenBank | EAY98715.1 | 0 | 126 | 837 | 1 | 712 | hypothetical protein OsI_20647 [Oryza sativa Indica Group] |
| RefSeq | NP_001123593.2 | 0 | 1 | 837 | 1 | 802 | trehalose-6-phosphate synthase [Zea mays] |
| RefSeq | XP_002331300.1 | 0 | 117 | 837 | 76 | 795 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002440086.1 | 0 | 1 | 837 | 24 | 837 | hypothetical protein SORBIDRAFT_09g025790 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2wtx_D | 0 | 129 | 605 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_C | 0 | 129 | 605 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_B | 0 | 129 | 605 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 2wtx_A | 0 | 129 | 605 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
| PDB | 1uqu_B | 0 | 129 | 605 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| trehalose biosynthesis I | TREHALOSE6PSYN-RXN | EC-2.4.1.15 | α,α-trehalose-phosphate synthase (UDP-forming) |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CB651133 | 281 | 155 | 435 | 0 |
| CB629539 | 271 | 307 | 577 | 0 |
| DW069910 | 288 | 215 | 502 | 0 |
| EH189441 | 291 | 497 | 787 | 0 |
| CV711307 | 293 | 127 | 419 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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