| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os06g26234.1 |
| Family | GH13 |
| Protein Properties | Length: 904 Molecular Weight: 103836 Isoelectric Point: 5.8574 |
| Chromosome | Chromosome/Scaffold: 6 Start: 15335687 End: 15365531 |
| Description | Alpha amylase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 425 | 686 | 2.1e-24 |
| EFTSNVLPHIKDAGYNAIQLIGIVEHKDYSSVGYKVTNYFSVSSRFGSPDDFKKLVDEAHGLGLVVLLDIVHSYASADELVGLSLFDGSNDCYFHSGKRG HHKYWGTRMFKYDDIDVLHFLLSNLNWWVTEYRVDGFQFHSLPSMLYTHNGFSTFTGATEEYYNQYVDEDALIYLIIANEMLHELHPDIITIAEDATFYP GLCEPTTQGGLGFDYWVNLSIPEMWLWHLENVPEQEWSMNKIMRVLVNNNSNMLSYVENHNQ | |||
| Full Sequence |
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| Protein Sequence Length: 904 Download |
| MDSCALFLST PRPPPPLVPA HRRRPLASRS GLRRREGCPC SCASSSSSSG RAGSQDRPPR 60 PWQQKQRTQR PGRGEAIDPV GFLAKHGISD RAFAQFLRDR YKALKDRRWE LHSRLIDLKE 120 ASSGFELMGM HRHRQHRVDF MEWAPGARYC SVVGDFNQWS TTENCAREGH LGHDDFGYWT 180 IILEDKLREG QEPDEYYFQE YNYADDYDKG DNGVDVEELI HRMNEEYWEP GEIKSQKSRL 240 EVVAKLYEQM FGPNGPQTEE ELGDIPDAET RYNEWKALQK DDSASSLPCY DIIDNGQEFD 300 IFNVATDRVS FEKFQGKSPP LAYWVEMRKG RKAWLEKYVP AISHKDKYRV YFNTPDGGLE 360 RIPAWATYVL PDAEGKQSYA VHWDPPPEEI YKWRFERPKV KGSLRIYECH VGISGSEQKI 420 SSFQEFTSNV LPHIKDAGYN AIQLIGIVEH KDYSSVGYKV TNYFSVSSRF GSPDDFKKLV 480 DEAHGLGLVV LLDIVHSYAS ADELVGLSLF DGSNDCYFHS GKRGHHKYWG TRMFKYDDID 540 VLHFLLSNLN WWVTEYRVDG FQFHSLPSML YTHNGFSTFT GATEEYYNQY VDEDALIYLI 600 IANEMLHELH PDIITIAEDA TFYPGLCEPT TQGGLGFDYW VNLSIPEMWL WHLENVPEQE 660 WSMNKIMRVL VNNNSNMLSY VENHNQSISG RKSFAEIILY EGKCSNSSVD NDLIFRASSL 720 LNIIKLITFT TSGGAYLNFI GNEFAHPKRI EFPMSSNDYS FCLANRQWEL LDKGVHKHIF 780 NFDKDIMSLD GKERLISGGS PIVHHCDDTS MIIYFTRGPF LFVFNFNPDA SYQLYSVGVD 840 EAGEYQLILN TDETKYGGRG ELTSNQYMKR TSDNRVGGCR NSLELTLPSR SAQVFKLVRI 900 LRI* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02447 | PLN02447 | 9.0e-11 | 91 | 185 | 95 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 7.0e-167 | 387 | 784 | 406 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02960 | PLN02960 | 0 | 1 | 903 | 908 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 0 | 59 | 903 | 853 | + alpha-amylase; Provisional | ||
| PLN02447 | PLN02447 | 0 | 339 | 898 | 575 | + 1,4-alpha-glucan-branching enzyme | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0000003 | reproduction |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005488 | binding |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI26672.1 | 0 | 60 | 903 | 49 | 896 | unnamed protein product [Vitis vinifera] |
| RefSeq | NP_001057611.1 | 0 | 1 | 903 | 1 | 903 | Os06g0367100 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001108121.1 | 0 | 1 | 903 | 1 | 899 | starch branching enzyme III [Zea mays] |
| RefSeq | NP_001154629.1 | 0 | 76 | 903 | 68 | 899 | alpha-amylase/ catalytic/ cation binding [Arabidopsis thaliana] |
| RefSeq | XP_002455921.1 | 0 | 16 | 903 | 24 | 906 | hypothetical protein SORBIDRAFT_03g027310 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3amk_A | 0 | 340 | 898 | 114 | 693 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 340 | 898 | 114 | 693 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 340 | 898 | 114 | 693 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CB644230 | 255 | 125 | 379 | 0 |
| CB644186 | 245 | 125 | 369 | 0 |
| JG953387 | 280 | 511 | 790 | 0 |
| BF272517 | 278 | 361 | 638 | 0 |
| JG636329 | 270 | 348 | 617 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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