| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os06g51050.1 |
| Family | GH19 |
| Protein Properties | Length: 321 Molecular Weight: 33681.4 Isoelectric Point: 4.6364 |
| Chromosome | Chromosome/Scaffold: 6 Start: 30887215 End: 30888471 |
| Description | basic chitinase |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH19 | 76 | 311 | 0 |
| VASIISPSLFDQMLLHRNDQACAAKGFYTYDAFVAAANAYPDFATTGDADTCKREVAAFLAQTSHETTGGWPTAPDGPYSWGYCFKEENNGNAPTYCEPK PEWPCAAGKKYYGRGPIQITYNYNYGPAGQAIGSDLLNNPDLVASDATVSFKTAFWFWMTPQSPKPSCHAVITGQWTPSADDQAAGRVPGYGEITNIING GVECGHGADDKVADRIGFYKRYCDMLGVSYGDNLDC | |||
| Full Sequence |
|---|
| Protein Sequence Length: 321 Download |
| MRALALAVVA MAVVAVRGEQ CGSQAGGALC PNCLCCSQYG WCGSTSDYCG AGCQSQCSGG 60 CGGGPTPPSS GGGSGVASII SPSLFDQMLL HRNDQACAAK GFYTYDAFVA AANAYPDFAT 120 TGDADTCKRE VAAFLAQTSH ETTGGWPTAP DGPYSWGYCF KEENNGNAPT YCEPKPEWPC 180 AAGKKYYGRG PIQITYNYNY GPAGQAIGSD LLNNPDLVAS DATVSFKTAF WFWMTPQSPK 240 PSCHAVITGQ WTPSADDQAA GRVPGYGEIT NIINGGVECG HGADDKVADR IGFYKRYCDM 300 LGVSYGDNLD CYNQRPYPPS * 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00187 | Chitin_bind_1 | 4.0e-10 | 20 | 48 | 29 | + Chitin recognition protein. | ||
| cd06921 | ChtBD1_GH19_hevein | 8.0e-11 | 19 | 48 | 30 | + Hevein or Type 1 chitin binding domain subfamily co-occuring with family 19 glycosyl hydrolases or with barwin domains. This subfamily includes Hevein, a major IgE-binding allergen in natural rubber latex. ChtBD1 is a lectin domain found in proteins from plants and fungi that bind N-acetylglucosamine, plant endochitinases, wound-induced proteins, and the alpha subunit of Kluyveromyces lactis killer toxin. This domain is involved in the recognition and/or binding of chitin subunits; it typically occurs N-terminal to glycosyl hydrolase domains in chitinases, together with other carbohydrate-binding domains, or by itself in tandem-repeat arrangements. | ||
| cd00442 | lysozyme_like | 1.0e-16 | 132 | 273 | 142 | + lysozyme_like domain. This contains several members including Soluble Lytic Transglycosylases (SLT), Goose Egg-White Lysozymes (GEWL), Hen Egg-White Lysozymes (HEWL), chitinases, bacteriophage lambda lysozymes, endolysins, autolysins, and chitosanases. All the members are involved in the hydrolysis of beta-1,4- linked polysaccharides. | ||
| cd00325 | chitinase_glyco_hydro_19 | 2.0e-141 | 84 | 311 | 228 | + Glycoside hydrolase family 19 chitinase domain. Chitinases are enzymes that catalyze the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. Family 19 chitinases are found primarily in plants (classes I, III, and IV), but some are found in bacteria. Class I and II chitinases are similar in their catalytic domains. Class I chitinases have an N-terminal cysteine-rich, chitin-binding domain which is separated from the catalytic domain by a proline and glycine-rich hinge region. Class II chitinases lack both the chitin-binding domain and the hinge region. Class IV chitinases are similar to class I chitinases but they are smaller in size due to certain deletions. Despite any significant sequence homology with lysozymes, structural analysis reveals that family 19 chitinases, together with family 46 chitosanases, are similar to several lysozymes including those from T4-phage and from goose. The structures reveal that the different enzyme groups arose from a common ancestor glycohydrolase antecedent to the procaryotic/eucaryotic divergence. | ||
| pfam00182 | Glyco_hydro_19 | 1.0e-151 | 84 | 311 | 228 | + Chitinase class I. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004568 | chitinase activity |
| GO:0005773 | vacuole |
| GO:0005829 | cytosol |
| GO:0006032 | chitin catabolic process |
| GO:0006950 | response to stress |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAA38249.1 | 0 | 1 | 320 | 1 | 318 | endochitinase [Oryza sativa] |
| EMBL | CAA82849.1 | 0 | 1 | 317 | 1 | 317 | chitinase class I [Oryza sativa] |
| EMBL | CAA82850.1 | 0 | 19 | 320 | 18 | 308 | chitinase class I [Oryza sativa] |
| GenBank | EAZ02415.1 | 0 | 1 | 320 | 1 | 320 | hypothetical protein OsI_24517 [Oryza sativa Indica Group] |
| RefSeq | NP_001058626.1 | 0 | 1 | 320 | 1 | 320 | Os06g0726100 [Oryza sativa (japonica cultivar-group)] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3iwr_B | 0 | 19 | 317 | 2 | 298 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 3iwr_A | 0 | 19 | 317 | 2 | 298 | A Chain A, Solution Structure Of The C-Terminal Domain Ole E 9 |
| PDB | 2dkv_A | 0 | 19 | 317 | 2 | 298 | A Chain A, Crystal Structure Of Class I Chitinase From Oryza Sativa L. Japonica |
| PDB | 4dyg_B | 0 | 76 | 317 | 3 | 243 | A Chain A, Crystal Structure Of Class I Chitinase From Oryza Sativa L. Japonica |
| PDB | 4dyg_A | 0 | 76 | 317 | 3 | 243 | A Chain A, Crystal Structure Of Class I Chitinase From Oryza Sativa L. Japonica |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| chitin degradation II | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12623 | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12624 | EC-3.2.1.14 | chitinase |
| chitin degradation III (carnivorous plants) | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
