| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os06g51084.4 |
| Family | GH13 |
| Protein Properties | Length: 756 Molecular Weight: 86634.7 Isoelectric Point: 6.3797 |
| Chromosome | Chromosome/Scaffold: 6 Start: 30897376 End: 30905803 |
| Description | starch branching enzyme 2.2 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 208 | 533 | 8.4e-28 |
| LPRIRANNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLVDKAHSLGLRVLMDVVHSHASNNVTDGLNGYDVGQNTHESYFHTGDRGYHK LWDSRLFNYANWEVLRFLLSNLRYWMDEFMFDGFRFDGVTSMLYHHHGINKGFTGNYKEYFSLDTDVDAIVYMMLANHLMHKLLPEATIVAEDVSGMPVL CRPVDEGGVGFDFRLAMAIPDRWIDYLKNKEDRKWSMSEIVQTLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLMDKEMYTGMSDLQPASPTINRGIALQ KMIHFITMALGGDGYLNFMGNEFGHP | |||
| Full Sequence |
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| Protein Sequence Length: 756 Download |
| MVTVVEEVDH LPIYDLDPKL EEFKDHFNYR IKRYLDQKCL IEKHEGGLEE FSKGYLKFGI 60 NTVDGATIYR EWAPAAQEAQ LIGEFNNWNG AKHKMEKDKF GIWSIKISHV NGKPAIPHNS 120 KVKFRFRHGG GAWVDRIPAW IRYATFDASK FGAPYDGVHW DPPACERYVF KHPRPPKPDA 180 PRIYEAHVGM SGEEPEVSTY REFADNVLPR IRANNYNTVQ LMAIMEHSYY ASFGYHVTNF 240 FAVSSRSGTP EDLKYLVDKA HSLGLRVLMD VVHSHASNNV TDGLNGYDVG QNTHESYFHT 300 GDRGYHKLWD SRLFNYANWE VLRFLLSNLR YWMDEFMFDG FRFDGVTSML YHHHGINKGF 360 TGNYKEYFSL DTDVDAIVYM MLANHLMHKL LPEATIVAED VSGMPVLCRP VDEGGVGFDF 420 RLAMAIPDRW IDYLKNKEDR KWSMSEIVQT LTNRRYTEKC IAYAESHDQS IVGDKTIAFL 480 LMDKEMYTGM SDLQPASPTI NRGIALQKMI HFITMALGGD GYLNFMGNEF GHPEWIDFPR 540 EGNNWSYDKC RRQWSLVDTD HLRYKYMNAF DQAMNALEEE FSFLSSSKQI VSDMNEKDKV 600 IVFERGDLVF VFNFHPNKTY KGYKVGCDLP GKYRVALDSD ALVFGGHGRV GHDVDHFTSP 660 EGMPGVPETN FNNRPNSFKV LSPPRTCVAY YRVDEDREEL RRGGAVASGK IVTEYIDVEA 720 TSGETISGGW KGSEKDDCGK KGMKFVFRSS DEDCK* 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PLN02960 | PLN02960 | 3.0e-5 | 30 | 107 | 84 | + alpha-amylase | ||
| PLN03244 | PLN03244 | 2.0e-132 | 114 | 640 | 529 | + alpha-amylase; Provisional | ||
| PLN02960 | PLN02960 | 2.0e-176 | 114 | 640 | 528 | + alpha-amylase | ||
| PLN02447 | PLN02447 | 0 | 1 | 720 | 720 | + 1,4-alpha-glucan-branching enzyme | ||
| cd11321 | AmyAc_bac_euk_BE | 0 | 166 | 572 | 407 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| GO:0009058 | biosynthetic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAP68993.1 | 0 | 1 | 755 | 66 | 820 | starch-branching enzyme 1 [Oryza sativa Japonica Group] |
| GenBank | ABL74558.1 | 0 | 1 | 755 | 64 | 818 | starch-branching enzyme I [Oryza sativa Japonica Group] |
| DDBJ | BAA01584.1 | 0 | 1 | 755 | 66 | 820 | branching enzyme [Oryza sativa Japonica Group] |
| DDBJ | BAD61713.1 | 0 | 1 | 755 | 1 | 755 | putative 1,4-alpha-glucan branching enzyme I precursor [Oryza sativa Japonica Group] |
| RefSeq | NP_001058629.1 | 0 | 1 | 755 | 66 | 820 | Os06g0726400 [Oryza sativa (japonica cultivar-group)] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3aml_A | 0 | 1 | 755 | 1 | 755 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3amk_A | 0 | 1 | 702 | 1 | 702 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
| PDB | 3vu2_B | 0 | 1 | 702 | 1 | 702 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 3vu2_A | 0 | 1 | 702 | 1 | 702 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
| PDB | 1m7x_D | 1.00053e-42 | 61 | 646 | 19 | 577 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO619167 | 614 | 97 | 709 | 0 |
| CX109187 | 447 | 310 | 756 | 0 |
| HO794536 | 695 | 13 | 695 | 0 |
| HO777638 | 643 | 65 | 695 | 0 |
| HO777638 | 44 | 16 | 59 | 0.37 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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