Basic Information | |
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Species | Oryza sativa |
Cazyme ID | LOC_Os06g51084.6 |
Family | GH13 |
Protein Properties | Length: 819 Molecular Weight: 93009 Isoelectric Point: 6.7581 |
Chromosome | Chromosome/Scaffold: 6 Start: 30897376 End: 30905801 |
Description | starch branching enzyme 2.2 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 271 | 596 | 1.1e-27 |
LPRIRANNYNTVQLMAIMEHSYYASFGYHVTNFFAVSSRSGTPEDLKYLVDKAHSLGLRVLMDVVHSHASNNVTDGLNGYDVGQNTHESYFHTGDRGYHK LWDSRLFNYANWEVLRFLLSNLRYWMDEFMFDGFRFDGVTSMLYHHHGINKGFTGNYKEYFSLDTDVDAIVYMMLANHLMHKLLPEATIVAEDVSGMPVL CRPVDEGGVGFDFRLAMAIPDRWIDYLKNKEDRKWSMSEIVQTLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLMDKEMYTGMSDLQPASPTINRGIALQ KMIHFITMALGGDGYLNFMGNEFGHP |
Full Sequence |
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Protein Sequence Length: 819 Download |
MLCLTSSSSS APAPLLPSLA DRPSPGIAGG GGNVRLSVVS SPRRSWPGKT NFSVPATARK 60 NKTMVTVVEE VDHLPIYDLD PKLEEFKDHF NYRIKRYLDQ KCLIEKHEGG LEEFSKGYLK 120 FGINTVDGAT IYREWAPAAQ EAQLIGEFNN WNGAKHKMEK DKFGIWSIKI SHVNGKPAIP 180 HNSKVKFRFR HGGGAWVDRI PAWIRYATFD ASKFGAPYDG VHWDPPACER YVFKHPRPPK 240 PDAPRIYEAH VGMSGEEPEV STYREFADNV LPRIRANNYN TVQLMAIMEH SYYASFGYHV 300 TNFFAVSSRS GTPEDLKYLV DKAHSLGLRV LMDVVHSHAS NNVTDGLNGY DVGQNTHESY 360 FHTGDRGYHK LWDSRLFNYA NWEVLRFLLS NLRYWMDEFM FDGFRFDGVT SMLYHHHGIN 420 KGFTGNYKEY FSLDTDVDAI VYMMLANHLM HKLLPEATIV AEDVSGMPVL CRPVDEGGVG 480 FDFRLAMAIP DRWIDYLKNK EDRKWSMSEI VQTLTNRRYT EKCIAYAESH DQSIVGDKTI 540 AFLLMDKEMY TGMSDLQPAS PTINRGIALQ KMIHFITMAL GGDGYLNFMG NEFGHPEWID 600 FPREGNNWSY DKCRRQWSLV DTDHLRYKYM NAFDQAMNAL EEEFSFLSSS KQIVSDMNEK 660 DKVIVFERGD LVFVFNFHPN KTYKGYKVGC DLPGKYRVAL DSDALVFGGH GRVGHDVDHF 720 TSPEGMPGVP ETNFNNRPNS FKVLSPPRTC VAYYRVDEDR EELRRGGAVA SGKIVTEYID 780 VEATSGETIS GGWKGSEKDD CGKKGMKFVF RSSDEDCK* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02960 | PLN02960 | 3.0e-5 | 93 | 170 | 84 | + alpha-amylase | ||
PLN03244 | PLN03244 | 4.0e-132 | 177 | 703 | 529 | + alpha-amylase; Provisional | ||
PLN02960 | PLN02960 | 3.0e-176 | 177 | 703 | 528 | + alpha-amylase | ||
PLN02447 | PLN02447 | 0 | 21 | 783 | 765 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 229 | 635 | 407 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0008152 | metabolic process |
GO:0009058 | biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAP68993.1 | 0 | 1 | 818 | 1 | 820 | starch-branching enzyme 1 [Oryza sativa Japonica Group] |
GenBank | ABL74558.1 | 0 | 1 | 818 | 1 | 818 | starch-branching enzyme I [Oryza sativa Japonica Group] |
DDBJ | BAA01584.1 | 0 | 1 | 818 | 1 | 820 | branching enzyme [Oryza sativa Japonica Group] |
GenBank | EEC81337.1 | 0 | 1 | 818 | 1 | 827 | hypothetical protein OsI_24520 [Oryza sativa Indica Group] |
RefSeq | NP_001058629.1 | 0 | 1 | 818 | 1 | 820 | Os06g0726400 [Oryza sativa (japonica cultivar-group)] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3aml_A | 0 | 64 | 818 | 1 | 755 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3amk_A | 0 | 64 | 765 | 1 | 702 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 64 | 765 | 1 | 702 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 64 | 765 | 1 | 702 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 1m7x_D | 1.99965e-42 | 124 | 709 | 19 | 577 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch biosynthesis | RXN-7710 | EC-2.4.1.18 | 1,4-α-glucan branching enzyme |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO619167 | 614 | 160 | 772 | 0 |
CX109187 | 447 | 373 | 819 | 0 |
HO794536 | 695 | 76 | 758 | 0 |
HO777638 | 639 | 132 | 758 | 0 |
HO777638 | 44 | 79 | 122 | 0.34 |
Sequence Alignments (This image is cropped. Click for full image.) |
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