| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os07g23850.1 |
| Family | GH18 |
| Protein Properties | Length: 270 Molecular Weight: 28739.7 Isoelectric Point: 9.4852 |
| Chromosome | Chromosome/Scaffold: 7 Start: 13470399 End: 13471208 |
| Description | chitinase A |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH18 | 26 | 224 | 1.4e-24 |
| TVIISFLTDFGGGNYKLNLAGHAWSAVGPDVKYCRSKGVLVLLSIGGGVGRYSLASQADAKAVADHLWNFYLGGTSTMSWPFGDAVLDGVDFDIELGSNA HYGDLARYLKAYSGRKPGGRKVWLTAAPQCPFPDRMLGEALRTGLFDRVHVQFYNNPVCNYRASNVAAFTSAWNKWAASLPGSSVYLGLPAVSGAANNG | |||
| Full Sequence |
|---|
| Protein Sequence Length: 270 Download |
| MPCGYGVPII STGGLREACG TGLYSTVIIS FLTDFGGGNY KLNLAGHAWS AVGPDVKYCR 60 SKGVLVLLSI GGGVGRYSLA SQADAKAVAD HLWNFYLGGT STMSWPFGDA VLDGVDFDIE 120 LGSNAHYGDL ARYLKAYSGR KPGGRKVWLT AAPQCPFPDR MLGEALRTGL FDRVHVQFYN 180 NPVCNYRASN VAAFTSAWNK WAASLPGSSV YLGLPAVSGA ANNGYVAPAT LKENVLPIVQ 240 KSKNYGGIML WSRNWDKQTG YSKSVKSAV* 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00598 | GH18_chitinase-like | 0.008 | 1 | 256 | 270 | + The GH18 (glycosyl hydrolase, family 18) type II chitinases hydrolyze chitin, an abundant polymer of beta-1,4-linked N-acetylglucosamine (GlcNAc) which is a major component of the cell wall of fungi and the exoskeleton of arthropods. Chitinases have been identified in viruses, bacteria, fungi, protozoan parasites, insects, and plants. The structure of the GH18 domain is an eight-stranded beta/alpha barrel with a pronounced active-site cleft at the C-terminal end of the beta-barrel. The GH18 family includes chitotriosidase, chitobiase, hevamine, zymocin-alpha, narbonin, SI-CLP (stabilin-1 interacting chitinase-like protein), IDGF (imaginal disc growth factor), CFLE (cortical fragment-lytic enzyme) spore hydrolase, the type III and type V plant chitinases, the endo-beta-N-acetylglucosaminidases, and the chitolectins. The GH85 (glycosyl hydrolase, family 85) ENGases (endo-beta-N-acetylglucosaminidases) are closely related to the GH18 chitinases and are included in this alignment model. | ||
| COG3469 | COG3469 | 4.0e-8 | 113 | 259 | 176 | + Chitinase [Carbohydrate transport and metabolism] | ||
| pfam00704 | Glyco_hydro_18 | 1.0e-9 | 12 | 256 | 325 | + Glycosyl hydrolases family 18. | ||
| cd02871 | GH18_chitinase_D-like | 5.0e-11 | 112 | 266 | 207 | + GH18 domain of Chitinase D (ChiD). ChiD, a chitinase found in Bacillus circulans, hydrolyzes the 1,4-beta-linkages of N-acetylglucosamine in chitin and chitodextrins. The domain architecture of ChiD includes a catalytic glycosyl hydrolase family 18 (GH18) domain, a chitin-binding domain, and a fibronectin type III domain. The chitin-binding and fibronectin type III domains are located either N-terminal or C-terminal to the catalytic domain. This family includes exochitinase Chi36 from Bacillus cereus. | ||
| cd02877 | GH18_hevamine_XipI_class_III | 5.0e-107 | 12 | 267 | 269 | + This conserved domain family includes xylanase inhibitor Xip-I, and the class III plant chitinases such as hevamine, concanavalin B, and PPL2, all of which have a glycosyl hydrolase family 18 (GH18) domain. Hevamine is a class III endochitinase that hydrolyzes the linear polysaccharide chains of chitin and peptidoglycan and is important for defense against pathogenic bacteria and fungi. PPL2 (Parkia platycephala lectin 2) is a class III chitinase from Parkia platycephala seeds that hydrolyzes beta(1-4) glycosidic bonds linking 2-acetoamido-2-deoxy-beta-D-glucopyranose units in chitin. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005488 | binding |
| GO:0005623 | cell |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAD56239.1 | 0 | 11 | 269 | 39 | 301 | AF184884_1 class III chitinase [Benincasa hispida] |
| DDBJ | BAC10165.1 | 0 | 1 | 269 | 1 | 269 | putative class III chitinase [Oryza sativa Japonica Group] |
| EMBL | CAD40352.2 | 0 | 11 | 269 | 44 | 301 | OSJNBa0020I02.6 [Oryza sativa (japonica cultivar-group)] |
| GenBank | EAZ03635.1 | 0 | 11 | 269 | 43 | 301 | hypothetical protein OsI_25770 [Oryza sativa Indica Group] |
| GenBank | EEE67049.1 | 0 | 1 | 269 | 20 | 288 | hypothetical protein OsJ_23994 [Oryza sativa Japonica Group] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2hvm_A | 0 | 11 | 269 | 12 | 273 | A Chain A, Peanut Peroxidase |
| PDB | 1llo_A | 0 | 11 | 269 | 12 | 273 | A Chain A, Peanut Peroxidase |
| PDB | 1hvq_A | 0 | 11 | 269 | 12 | 273 | A Chain A, Crystal Structures Of Hevamine, A Plant Defence Protein With Chitinase And Lysozyme Activity, And Its Complex With An Inhibitor |
| PDB | 1kr0_A | 0 | 11 | 269 | 12 | 273 | A Chain A, Hevamine Mutant D125aY183F IN COMPLEX WITH TETRA-Nag |
| PDB | 1kr1_A | 0 | 11 | 269 | 12 | 273 | A Chain A, Hevamine Mutant D125aE127A IN COMPLEX WITH TETRA-Nag |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| chitin degradation II | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12623 | EC-3.2.1.14 | chitinase |
| chitin degradation II | RXN-12624 | EC-3.2.1.14 | chitinase |
| chitin degradation III (carnivorous plants) | 3.2.1.14-RXN | EC-3.2.1.14 | chitinase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EE591240 | 260 | 11 | 270 | 0 |
| BE040022 | 177 | 30 | 206 | 0 |
| GT039390 | 258 | 13 | 270 | 0 |
| CJ595937 | 197 | 76 | 270 | 0 |
| BE040022 | 37 | 204 | 240 | 0.004 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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