| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os08g06180.1 |
| Family | AA7 |
| Protein Properties | Length: 355 Molecular Weight: 39491.8 Isoelectric Point: 8.9334 |
| Chromosome | Chromosome/Scaffold: 8 Start: 3412330 End: 3413394 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 1 | 349 | 0 |
| MGGFVSGGGFGLISRKFALAADNVLDAILMDPNGNALNRGSMGDDVFWAIRGGGGGSWGVVYAWKLQLVHVPHSITVFSLNRTGPLEQTAKLMHKWQFVG PHLPDEFYLSIHILTGTSNGNVSMSFTGQVIGPKQYAMLVLHHTFPELGIVESDLSEMSWIESTAKFARLNSTADLTNRRLGIKYYSKSKSDYVHSPISM QDTIKIIEYLSNGPQGFIQLNPYGGAMARIGSSELPFPYRAGYLYSIEYNVSWKASDNDRADEFIRWLRSFYAYMAPHVSKNPLAAYVNYLDLDLGTNTW RNATDGTSNNSVIHAKSWGIRYFSKNFDRLVRAKTMIDPENVFNNAQSI | |||
| Full Sequence |
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| Protein Sequence Length: 355 Download |
| MGGFVSGGGF GLISRKFALA ADNVLDAILM DPNGNALNRG SMGDDVFWAI RGGGGGSWGV 60 VYAWKLQLVH VPHSITVFSL NRTGPLEQTA KLMHKWQFVG PHLPDEFYLS IHILTGTSNG 120 NVSMSFTGQV IGPKQYAMLV LHHTFPELGI VESDLSEMSW IESTAKFARL NSTADLTNRR 180 LGIKYYSKSK SDYVHSPISM QDTIKIIEYL SNGPQGFIQL NPYGGAMARI GSSELPFPYR 240 AGYLYSIEYN VSWKASDNDR ADEFIRWLRS FYAYMAPHVS KNPLAAYVNY LDLDLGTNTW 300 RNATDGTSNN SVIHAKSWGI RYFSKNFDRL VRAKTMIDPE NVFNNAQSIP PLQY* 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 0.008 | 14 | 352 | 351 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam08031 | BBE | 1.0e-14 | 286 | 350 | 65 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003674 | molecular_function |
| GO:0003824 | catalytic activity |
| GO:0005488 | binding |
| GO:0005575 | cellular_component |
| GO:0005618 | cell wall |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAC65011.1 | 0 | 1 | 354 | 49 | 402 | putative berberine bridge enzyme [Oryza sativa Japonica Group] |
| GenBank | EAZ05659.1 | 0 | 12 | 352 | 187 | 528 | hypothetical protein OsI_27886 [Oryza sativa Indica Group] |
| GenBank | EAZ05660.1 | 0 | 1 | 354 | 1 | 354 | hypothetical protein OsI_27887 [Oryza sativa Indica Group] |
| GenBank | EAZ41577.1 | 0 | 1 | 354 | 1 | 354 | hypothetical protein OsJ_26111 [Oryza sativa Japonica Group] |
| RefSeq | NP_001061035.1 | 0 | 12 | 352 | 187 | 528 | Os08g0158200 [Oryza sativa (japonica cultivar-group)] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3fw9_A | 0 | 12 | 352 | 157 | 494 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With (S)-Scoulerine |
| PDB | 3fw8_A | 0 | 12 | 352 | 157 | 494 | A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant |
| PDB | 3fw7_A | 0 | 12 | 352 | 160 | 497 | A Chain A, Structure Of Berberine Bridge Enzyme, H104a Variant |
| PDB | 3fwa_A | 0 | 12 | 352 | 157 | 494 | A Chain A, Structure Of Berberine Bridge Enzyme, C166a Variant In Complex With (S)-Reticuline |
| PDB | 4ec3_A | 0 | 12 | 352 | 163 | 500 | A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In Complex With (S)-Reticuline |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| berberine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| dehydroscoulerine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| sanguinarine and macarpine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EG380703 | 211 | 143 | 352 | 0 |
| ES434378 | 250 | 12 | 261 | 0 |
| CA755054 | 205 | 57 | 261 | 0 |
| HX082799 | 197 | 157 | 352 | 0 |
| EL451713 | 294 | 67 | 352 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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