| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os08g13920.1 |
| Family | GH16 |
| Protein Properties | Length: 291 Molecular Weight: 32096.6 Isoelectric Point: 5.2995 |
| Chromosome | Chromosome/Scaffold: 8 Start: 8327866 End: 8329399 |
| Description | xyloglucan endotransglucosylase/hydrolase 25 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH16 | 40 | 209 | 7.4e-29 |
| HVRVVSDDGKTQQVALTLDRSSGSGFTSKDTYLFGEFSVQMKLVGGNSAGTVTSFYLSSGEGDGHDEIDIEFMGNLSGNPYVMNTNVWANGDGKKEHQFY LWFDPTADFHTYKIIWNPQNIIFQVDDVPVRTFKKYDDLAYPQSKPMRLHATLWDGSYWATRHGDVKIDW | |||
| Full Sequence |
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| Protein Sequence Length: 291 Download |
| MAKHLALSVA AAVAVSWLAA SSAAAAGFYE KFDVVGAGDH VRVVSDDGKT QQVALTLDRS 60 SGSGFTSKDT YLFGEFSVQM KLVGGNSAGT VTSFYLSSGE GDGHDEIDIE FMGNLSGNPY 120 VMNTNVWANG DGKKEHQFYL WFDPTADFHT YKIIWNPQNI IFQVDDVPVR TFKKYDDLAY 180 PQSKPMRLHA TLWDGSYWAT RHGDVKIDWS GAPFVVSYRG YSTNACVNNN PAGGWSSSWC 240 PEGTSAWIHR ELDGAELGTV AWAERNYMSY NYCADGWRFP QGFPAECYRK * 300 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd02175 | GH16_lichenase | 2.0e-21 | 32 | 196 | 174 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
| cd02183 | GH16_fungal_CRH1_transglycosylase | 2.0e-21 | 50 | 214 | 181 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
| pfam00722 | Glyco_hydro_16 | 1.0e-73 | 28 | 211 | 185 | + Glycosyl hydrolases family 16. | ||
| PLN03161 | PLN03161 | 1.0e-77 | 28 | 290 | 270 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
| cd02176 | GH16_XET | 1.0e-140 | 27 | 287 | 263 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005576 | extracellular region |
| GO:0005618 | cell wall |
| GO:0005623 | cell |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAT94293.1 | 0 | 27 | 290 | 25 | 283 | endotransglucosylase/hydrolase XTH1 [Triticum aestivum] |
| GenBank | AAT94294.1 | 0 | 27 | 290 | 25 | 283 | endotransglucosylase/hydrolase XTH2 [Triticum aestivum] |
| GenBank | EAZ06114.1 | 0 | 1 | 290 | 1 | 290 | hypothetical protein OsI_28350 [Oryza sativa Indica Group] |
| GenBank | EAZ14143.1 | 0 | 1 | 290 | 1 | 290 | hypothetical protein OsJ_04071 [Oryza sativa Japonica Group] |
| RefSeq | NP_001061319.1 | 0 | 1 | 290 | 1 | 290 | Os08g0237000 [Oryza sativa (japonica cultivar-group)] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1un1_B | 0 | 37 | 289 | 26 | 274 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
| PDB | 1un1_A | 0 | 37 | 289 | 26 | 274 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
| PDB | 1umz_B | 0 | 37 | 289 | 26 | 274 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 1umz_A | 0 | 37 | 289 | 26 | 274 | A Chain A, Xyloglucan Endotransglycosylase In Complex With The Xyloglucan Nonasaccharide Xllg. |
| PDB | 2uwb_B | 0 | 9 | 278 | 1 | 259 | A Chain A, Crystal Structure Of The Nasturtium Seedling Mutant Xyloglucanase Isoform Nxg1-Delta-Yniig |
| Transmembrane Domains | ||||
|---|---|---|---|---|
| Start | End | |||
| 7 | 29 | |||
| Signal Peptide | ||||
|---|---|---|---|---|
| Cleavage Site | ||||
| 25 | ||||
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CT842961 | 291 | 1 | 291 | 0 |
| CK208716 | 259 | 27 | 284 | 0 |
| DR736961 | 259 | 27 | 284 | 0 |
| HO060040 | 263 | 28 | 289 | 0 |
| BE040964 | 220 | 27 | 246 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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