| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os08g25490.1 |
| Family | AA7 |
| Protein Properties | Length: 518 Molecular Weight: 54406.5 Isoelectric Point: 9.3732 |
| Chromosome | Chromosome/Scaffold: 8 Start: 15510426 End: 15512115 |
| Description | FAD-binding Berberine family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 42 | 512 | 0 |
| RPAAVVLPRGRSELTSAVLCARHASLRIRVRSGAHSYEGLSYTVGDGGDDADRVRFVVIDLTRMNRVRVDAASATAWVESGATLGEIYYAVASSSSSLAF PAGSCSTVGAGGHISGGGFGLLSRKFKLAADNVLDAILVDADGRVLDRSSMGENVFWAIRGGGGGGWGVVYAWKLRLVQVPNTLTAFTPKRTGSVDAIAG LVHRWQYVGSALPDEFYLSVFLTIGGASSSSPSRDGNVTVSFTGLVLGSKELAMSVLSERFPELGLAEPEMSEMSWVESAARFAGLSSTEELTSRASRTK HYAKSKSDYVRSPIARGAVAAILRYLAGEPAGYVILDPYGGAMAREGSGDTPFPHRAGNLYSVQYGVTWEAGDDGGGGGGGGEARMAWLRALYAYMAPHV SKNPRAAYVNYVDLDLGTNALAGNVSSPSSSVSRARSTWGSAYFSPANFERLVGAKTLIDRSNVFSNAQSI | |||
| Full Sequence |
|---|
| Protein Sequence Length: 518 Download |
| MRNTTILLLA LLLTLLSSSS TWPPPLTGNL RFALPAAGAV PRPAAVVLPR GRSELTSAVL 60 CARHASLRIR VRSGAHSYEG LSYTVGDGGD DADRVRFVVI DLTRMNRVRV DAASATAWVE 120 SGATLGEIYY AVASSSSSLA FPAGSCSTVG AGGHISGGGF GLLSRKFKLA ADNVLDAILV 180 DADGRVLDRS SMGENVFWAI RGGGGGGWGV VYAWKLRLVQ VPNTLTAFTP KRTGSVDAIA 240 GLVHRWQYVG SALPDEFYLS VFLTIGGASS SSPSRDGNVT VSFTGLVLGS KELAMSVLSE 300 RFPELGLAEP EMSEMSWVES AARFAGLSST EELTSRASRT KHYAKSKSDY VRSPIARGAV 360 AAILRYLAGE PAGYVILDPY GGAMAREGSG DTPFPHRAGN LYSVQYGVTW EAGDDGGGGG 420 GGGEARMAWL RALYAYMAPH VSKNPRAAYV NYVDLDLGTN ALAGNVSSPS SSVSRARSTW 480 GSAYFSPANF ERLVGAKTLI DRSNVFSNAQ SIPPLQI* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG0277 | GlcD | 7.0e-7 | 60 | 380 | 332 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam08031 | BBE | 1.0e-11 | 448 | 513 | 66 | + Berberine and berberine like. This domain is found in the berberine bridge and berberine bridge- like enzymes which are involved in the biosynthesis of numerous isoquinoline alkaloids. They catalyze the transformation of the N-methyl group of (S)-reticuline into the C-8 berberine bridge carbon of (S)-scoulerine. | ||
| pfam01565 | FAD_binding_4 | 5.0e-12 | 53 | 190 | 139 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003674 | molecular_function |
| GO:0003824 | catalytic activity |
| GO:0005488 | binding |
| GO:0005623 | cell |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD03275.1 | 0 | 1 | 517 | 1 | 549 | putative berberine bridge enzyme [Oryza sativa Japonica Group] |
| DDBJ | BAF23509.2 | 0 | 1 | 517 | 1 | 553 | Os08g0343600 [Oryza sativa Japonica Group] |
| GenBank | EAZ42400.1 | 0 | 192 | 517 | 1 | 326 | hypothetical protein OsJ_26979 [Oryza sativa Japonica Group] |
| RefSeq | NP_001061595.1 | 0 | 29 | 517 | 69 | 557 | Os08g0343600 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002445086.1 | 0 | 29 | 515 | 69 | 559 | hypothetical protein SORBIDRAFT_07g003920 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3fw9_A | 0 | 40 | 515 | 43 | 494 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With (S)-Scoulerine |
| PDB | 4ec3_A | 0 | 40 | 515 | 49 | 500 | A Chain A, Structure Of Berberine Bridge Enzyme, H174a Variant In Complex With (S)-Reticuline |
| PDB | 3gsy_A | 0 | 40 | 515 | 49 | 500 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| PDB | 3d2j_A | 0 | 40 | 515 | 68 | 519 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| PDB | 3d2h_A | 0 | 40 | 515 | 68 | 519 | A Chain A, Structure Of Berberine Bridge Enzyme In Complex With Dehydroscoulerine |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| berberine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| dehydroscoulerine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
| sanguinarine and macarpine biosynthesis | RETICULINE-OXIDASE-RXN | EC-1.21.3.3 | reticuline oxidase |
