| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os08g35860.1 |
| Family | AA7 |
| Protein Properties | Length: 621 Molecular Weight: 66981.2 Isoelectric Point: 6.33 |
| Chromosome | Chromosome/Scaffold: 8 Start: 22616784 End: 22619732 |
| Description | cytokinin oxidase 7 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 39 | 233 | 7.9e-36 |
| GLVSARPAAVVRPASSDDVASAIRAAARTAHLTVAARGNGHSVAGQAMARGGLVLDMRALPRRMQLVVAPSGEKFADVPGGALWEEVLHWAVSKHGLAPA SWTDYLRLTVGGTLSNGGVSGQSFRYGPQVSNVAQLEVVTGDGECHVCSRSADPDLFFAVLGGLGQFGVITRARIPLSPAPQTVRWTRVVYASFA | |||
| Full Sequence |
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| Protein Sequence Length: 621 Download |
| MMLAYMDHAA AAAEPDAGAE PAVAAVDAAE FAAAMDFGGL VSARPAAVVR PASSDDVASA 60 IRAAARTAHL TVAARGNGHS VAGQAMARGG LVLDMRALPR RMQLVVAPSG EKFADVPGGA 120 LWEEVLHWAV SKHGLAPASW TDYLRLTVGG TLSNGGVSGQ SFRYGPQVSN VAQLEVVTGD 180 GECHVCSRSA DPDLFFAVLG GLGQFGVITR ARIPLSPAPQ TVRWTRVVYA SFADYAADAE 240 WLVTRPPHEA FDYVEGFAFV RSDDPVNGWP TVPIPDGAHF DASLLPANAG PVLYCLEVAL 300 YQRGGGGDGG GDDMDKRVGE MMRQLKYVRG LEFAAGVGYV DFLSRVNRVE DEARRNGSWA 360 APHPWLNLFI SSRDIAAFDR AVLNGMLADG VDGPMLIYPM LKSKWDPATS VALPNGEIFY 420 LVALLRFCRP YPGGGPPVDE LVAQNNAIID ACRSNGYDYK IYFPSYHAQS DWSRHFGAKW 480 SRFVDRKARY DPLAILAPGQ NIFARTPSSV AAAAAVIVMM VMMGMKRRDR DEHNDDGDDD 540 RVIVRYIWEG CKRGRFRFDG MDDVSRAVGP AAVARLCSRS CKRENERCKR FPALILFLLI 600 PPGEIGTPFD GDTHLVSLLG * 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01679 | bact_FAD_ox | 3.0e-7 | 76 | 271 | 202 | + FAD-linked oxidoreductase. This model represents a family of bacterial oxidoreductases with covalently linked FAD, closely related to two different eukaryotic oxidases, L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. | ||
| COG0277 | GlcD | 2.0e-21 | 76 | 502 | 433 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam01565 | FAD_binding_4 | 8.0e-22 | 76 | 187 | 112 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| pfam09265 | Cytokin-bind | 2.0e-137 | 219 | 503 | 287 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 76 | 509 | 437 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005575 | cellular_component |
| GO:0008152 | metabolic process |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009056 | catabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ADB81979.1 | 0 | 1 | 509 | 1 | 510 | cytokinin oxidase/dehydrogenase 3 [Triticum aestivum] |
| DDBJ | BAD09964.1 | 0 | 1 | 518 | 1 | 518 | putative cytokinin dehydrogenase [Oryza sativa Japonica Group] |
| GenBank | EEC83684.1 | 0 | 1 | 518 | 1 | 450 | hypothetical protein OsI_29488 [Oryza sativa Indica Group] |
| GenBank | EEE68814.1 | 0 | 124 | 518 | 41 | 435 | hypothetical protein OsJ_27576 [Oryza sativa Japonica Group] |
| RefSeq | NP_001146838.1 | 0 | 1 | 511 | 1 | 524 | cytokinin dehydrogenase 10 [Zea mays] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 28 | 506 | 45 | 523 | A Chain A, Crystal Structure Of Glycogen Debranching Enzyme Trex From Sulfolobus Solfataricus |
| PDB | 2exr_A | 0 | 28 | 506 | 45 | 523 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3s1d_A | 0 | 29 | 503 | 35 | 515 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3s1c_A | 0 | 29 | 503 | 35 | 515 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| PDB | 3dq0_A | 0 | 29 | 503 | 35 | 515 | A Chain A, Glu381ser Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenosine |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO781924 | 428 | 76 | 503 | 0 |
| CB673387 | 224 | 101 | 324 | 0 |
| EG398274 | 240 | 270 | 509 | 0 |
| ES791244 | 357 | 76 | 430 | 0 |
| FL714394 | 269 | 83 | 338 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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