| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os08g36910.3 |
| Family | GH13 |
| Protein Properties | Length: 397 Molecular Weight: 43443 Isoelectric Point: 5.7587 |
| Chromosome | Chromosome/Scaffold: 8 Start: 23340676 End: 23343533 |
| Description | alpha-amylase-like |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH13 | 80 | 384 | 1.80067e-42 |
| QGGWYNMLKGQVDDIAKAGVTHVWLPPPSHSVAPQGYMPGRLYDLDASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCAEKKDARGVYCVFEGGTPDD RLDWGPGMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPRVQRELTDWLNWLKSDVGFDGWRLDFAKGYSTDIAKMYVESCKPGFVVAEIWNSLSYNGD GKPAANQDQGRQELVNWVNAVGGPAMTFDFTTKGLLQAGVQGELWRLRDGNGKAAGMIGWLPEKAVTFVDNHDTGSTQKLWPFPSDKVMQGYAYILTHPG VPCIV | |||
| Full Sequence |
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| Protein Sequence Length: 397 Download |
| MPPDVEVIRH EHIDHPSSTR DRSVVVSSNS LSNTVSAYTD MKNTSSLCLL LLVVLCSLTC 60 NSGQAQVLFQ GFNWESWKQQ GGWYNMLKGQ VDDIAKAGVT HVWLPPPSHS VAPQGYMPGR 120 LYDLDASKYG TAAELKSLIA AFHGKGVQCV ADVVINHRCA EKKDARGVYC VFEGGTPDDR 180 LDWGPGMICS DDTQYSDGTG HRDTGEGFGA APDIDHLNPR VQRELTDWLN WLKSDVGFDG 240 WRLDFAKGYS TDIAKMYVES CKPGFVVAEI WNSLSYNGDG KPAANQDQGR QELVNWVNAV 300 GGPAMTFDFT TKGLLQAGVQ GELWRLRDGN GKAAGMIGWL PEKAVTFVDN HDTGSTQKLW 360 PFPSDKVMQG YAYILTHPGV PCIVSKPCII VLYTMS* 420 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 1.0e-45 | 64 | 383 | 395 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN02784 | PLN02784 | 9.0e-112 | 66 | 383 | 319 | + alpha-amylase | ||
| PLN02361 | PLN02361 | 3.0e-118 | 66 | 383 | 319 | + alpha-amylase | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 1.0e-145 | 67 | 383 | 320 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN00196 | PLN00196 | 0 | 61 | 383 | 326 | + alpha-amylase; Provisional | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005576 | extracellular region |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| GO:0009719 | response to endogenous stimulus |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAA33895.1 | 0 | 41 | 383 | 1 | 342 | alpha-amylase [Oryza sativa Japonica Group] |
| DDBJ | BAD73797.1 | 0 | 41 | 396 | 1 | 356 | putative alpha-amylase isozyme 3E precursor [Oryza sativa Japonica Group] |
| GenBank | EEC83728.1 | 0 | 41 | 381 | 1 | 341 | hypothetical protein OsI_29570 [Oryza sativa Indica Group] |
| RefSeq | NP_001062024.1 | 0 | 1 | 383 | 1 | 383 | Os08g0473900 [Oryza sativa (japonica cultivar-group)] |
| Swiss-Prot | P27933 | 0 | 41 | 383 | 1 | 343 | AMY3D_ORYSJ RecName: Full=Alpha-amylase isozyme 3D; AltName: Full=1,4-alpha-D-glucan glucanohydrolase; Flags: Precursor |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1bg9_A | 0 | 66 | 383 | 1 | 320 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 1ava_B | 0 | 66 | 383 | 1 | 320 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 1ava_A | 0 | 66 | 383 | 1 | 320 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 1amy_A | 0 | 66 | 383 | 1 | 320 | A Chain A, Amy2BASI PROTEIN-Protein Complex From Barley Seed |
| PDB | 3bsg_A | 0 | 66 | 383 | 2 | 322 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
| starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| CX109008 | 375 | 14 | 388 | 0 |
| FL870844 | 244 | 64 | 307 | 0 |
| FE614189 | 229 | 64 | 292 | 0 |
| DT767742 | 287 | 78 | 362 | 0 |
| EX262146 | 310 | 67 | 376 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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