| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os08g44020.2 |
| Family | PL4 |
| Protein Properties | Length: 659 Molecular Weight: 75086.4 Isoelectric Point: 4.8507 |
| Chromosome | Chromosome/Scaffold: 8 Start: 27714196 End: 27718701 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 25 | 641 | 0 |
| ATAGVSVKVSDRYVEIKNGIFELTLSNPDGIVTGVRYNGVDNLMEILNKEDNRGYWDLVWSKLGERTGIFDVIKGTEFRIIYQDENHAEVSFVRTWDPSL EGKAVPLNIDKRFIVLRGCSGFYTYGIYEHQEGWPGFSLGETRVAFKLRKDKFHYMALADDRQRIMPMPEDRVPPRGQQLAYPEAVLLVDPINPDLRGEV DDKYQYSCEDQYNNVHGWISFDPPIGFWQITPSDEFRTGGPVKQNLTSHVGPTMLAMFLSGHYAGDDLTPKFLTGEYWKKVHGPVFMYLNSSWDGSDPTL LWEDAKVQMMIEKESWPYCFALSDDFQKTEQRGCISGRLLVRDRYLDDADLYATSAYVGLALPGDVGSWQRECKGYQFWCRAEDDGSFCIRNIVAGDYNL YAWVPGFIGDYKLDAKLTISSGDDIYLGDLVYEPPRDGPTMWEIGIPDRSASEFFVPDPNPNYVNRLYINHPDRFRQYGLWERYAELYPDGDLVYTIGQS DYTTDWFFAQVNRRTDQSTYQPTTWQIKFNLDSVSPNSTYKFRVALASSANAELQVRFNDQDRTAPHFTTGLIGKDNTIARHGIHGLYWLFNIDVSGAWL VQGMNTIYLKQPRNQSP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 659 Download |
| MGKLSCKNLL PCCMGHPPAT SPAGATAGVS VKVSDRYVEI KNGIFELTLS NPDGIVTGVR 60 YNGVDNLMEI LNKEDNRGYW DLVWSKLGER TGIFDVIKGT EFRIIYQDEN HAEVSFVRTW 120 DPSLEGKAVP LNIDKRFIVL RGCSGFYTYG IYEHQEGWPG FSLGETRVAF KLRKDKFHYM 180 ALADDRQRIM PMPEDRVPPR GQQLAYPEAV LLVDPINPDL RGEVDDKYQY SCEDQYNNVH 240 GWISFDPPIG FWQITPSDEF RTGGPVKQNL TSHVGPTMLA MFLSGHYAGD DLTPKFLTGE 300 YWKKVHGPVF MYLNSSWDGS DPTLLWEDAK VQMMIEKESW PYCFALSDDF QKTEQRGCIS 360 GRLLVRDRYL DDADLYATSA YVGLALPGDV GSWQRECKGY QFWCRAEDDG SFCIRNIVAG 420 DYNLYAWVPG FIGDYKLDAK LTISSGDDIY LGDLVYEPPR DGPTMWEIGI PDRSASEFFV 480 PDPNPNYVNR LYINHPDRFR QYGLWERYAE LYPDGDLVYT IGQSDYTTDW FFAQVNRRTD 540 QSTYQPTTWQ IKFNLDSVSP NSTYKFRVAL ASSANAELQV RFNDQDRTAP HFTTGLIGKD 600 NTIARHGIHG LYWLFNIDVS GAWLVQGMNT IYLKQPRNQS PFQGLMYDYL RMEGPSGS* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd10316 | RGL4_M | 4.0e-29 | 355 | 454 | 100 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 5.0e-56 | 466 | 653 | 190 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 4.0e-72 | 29 | 324 | 301 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 1.0e-101 | 32 | 224 | 193 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005575 | cellular_component |
| GO:0008150 | biological_process |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD10227.1 | 0 | 14 | 658 | 1 | 645 | putative MYST1 [Oryza sativa Japonica Group] |
| GenBank | EEE69123.1 | 0 | 4 | 653 | 650 | 1299 | hypothetical protein OsJ_28233 [Oryza sativa Japonica Group] |
| RefSeq | NP_001062464.1 | 0 | 1 | 658 | 1 | 658 | Os08g0554100 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001062465.1 | 0 | 14 | 658 | 1 | 606 | Os08g0554300 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002445711.1 | 0 | 1 | 657 | 1 | 662 | hypothetical protein SORBIDRAFT_07g024560 [Sorghum bicolor] |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GO843311 | 311 | 5 | 315 | 0 |
| GO850432 | 302 | 5 | 306 | 0 |
| FE632882 | 246 | 116 | 361 | 0 |
| GO843500 | 255 | 405 | 659 | 0 |
| GO850432 | 25 | 307 | 331 | 0.87 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
