| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os08g44020.3 |
| Family | PL4 |
| Protein Properties | Length: 646 Molecular Weight: 73694.6 Isoelectric Point: 4.7634 |
| Chromosome | Chromosome/Scaffold: 8 Start: 27714525 End: 27718701 |
| Description | Rhamnogalacturonate lyase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| PL4 | 12 | 628 | 0 |
| ATAGVSVKVSDRYVEIKNGIFELTLSNPDGIVTGVRYNGVDNLMEILNKEDNRGYWDLVWSKLGERTGIFDVIKGTEFRIIYQDENHAEVSFVRTWDPSL EGKAVPLNIDKRFIVLRGCSGFYTYGIYEHQEGWPGFSLGETRVAFKLRKDKFHYMALADDRQRIMPMPEDRVPPRGQQLAYPEAVLLVDPINPDLRGEV DDKYQYSCEDQYNNVHGWISFDPPIGFWQITPSDEFRTGGPVKQNLTSHVGPTMLAMFLSGHYAGDDLTPKFLTGEYWKKVHGPVFMYLNSSWDGSDPTL LWEDAKVQMMIEKESWPYCFALSDDFQKTEQRGCISGRLLVRDRYLDDADLYATSAYVGLALPGDVGSWQRECKGYQFWCRAEDDGSFCIRNIVAGDYNL YAWVPGFIGDYKLDAKLTISSGDDIYLGDLVYEPPRDGPTMWEIGIPDRSASEFFVPDPNPNYVNRLYINHPDRFRQYGLWERYAELYPDGDLVYTIGQS DYTTDWFFAQVNRRTDQSTYQPTTWQIKFNLDSVSPNSTYKFRVALASSANAELQVRFNDQDRTAPHFTTGLIGKDNTIARHGIHGLYWLFNIDVSGAWL VQGMNTIYLKQPRNQSP | |||
| Full Sequence |
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| Protein Sequence Length: 646 Download |
| MGHPPATSPA GATAGVSVKV SDRYVEIKNG IFELTLSNPD GIVTGVRYNG VDNLMEILNK 60 EDNRGYWDLV WSKLGERTGI FDVIKGTEFR IIYQDENHAE VSFVRTWDPS LEGKAVPLNI 120 DKRFIVLRGC SGFYTYGIYE HQEGWPGFSL GETRVAFKLR KDKFHYMALA DDRQRIMPMP 180 EDRVPPRGQQ LAYPEAVLLV DPINPDLRGE VDDKYQYSCE DQYNNVHGWI SFDPPIGFWQ 240 ITPSDEFRTG GPVKQNLTSH VGPTMLAMFL SGHYAGDDLT PKFLTGEYWK KVHGPVFMYL 300 NSSWDGSDPT LLWEDAKVQM MIEKESWPYC FALSDDFQKT EQRGCISGRL LVRDRYLDDA 360 DLYATSAYVG LALPGDVGSW QRECKGYQFW CRAEDDGSFC IRNIVAGDYN LYAWVPGFIG 420 DYKLDAKLTI SSGDDIYLGD LVYEPPRDGP TMWEIGIPDR SASEFFVPDP NPNYVNRLYI 480 NHPDRFRQYG LWERYAELYP DGDLVYTIGQ SDYTTDWFFA QVNRRTDQST YQPTTWQIKF 540 NLDSVSPNST YKFRVALASS ANAELQVRFN DQDRTAPHFT TGLIGKDNTI ARHGIHGLYW 600 LFNIDVSGAW LVQGMNTIYL KQPRNQSPFQ GLMYDYLRME GPSGS* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd10316 | RGL4_M | 8.0e-29 | 342 | 441 | 100 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10317 | RGL4_C | 1.0e-55 | 453 | 640 | 190 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| cd10320 | RGL4_N | 2.0e-71 | 16 | 311 | 301 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
| pfam06045 | Rhamnogal_lyase | 3.0e-101 | 19 | 211 | 193 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0005575 | cellular_component |
| GO:0008150 | biological_process |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAD10227.1 | 0 | 1 | 645 | 1 | 645 | putative MYST1 [Oryza sativa Japonica Group] |
| GenBank | EEE69123.1 | 0 | 1 | 640 | 660 | 1299 | hypothetical protein OsJ_28233 [Oryza sativa Japonica Group] |
| RefSeq | NP_001062464.1 | 0 | 1 | 645 | 14 | 658 | Os08g0554100 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | NP_001062465.1 | 0 | 1 | 645 | 1 | 606 | Os08g0554300 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002445711.1 | 0 | 1 | 645 | 14 | 663 | hypothetical protein SORBIDRAFT_07g024560 [Sorghum bicolor] |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| GO843311 | 287 | 16 | 302 | 0 |
| GO850432 | 278 | 16 | 293 | 0 |
| FE632882 | 246 | 103 | 348 | 0 |
| GO843500 | 255 | 392 | 646 | 0 |
| GO850432 | 25 | 294 | 318 | 0.87 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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