Basic Information | |
---|---|
Species | Oryza sativa |
Cazyme ID | LOC_Os09g08072.1 |
Family | GH32 |
Protein Properties | Length: 596 Molecular Weight: 65349.6 Isoelectric Point: 6.5112 |
Chromosome | Chromosome/Scaffold: 9 Start: 4165397 End: 4170978 |
Description | beta-fructofuranosidase 5 |
View CDS |
External Links |
---|
NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH32 | 44 | 365 | 0 |
HFQPAKNWQNDAGPMYHNGMYHLFYQYNPHSALWDIGNLSWGHSVSGDLLNWAALDTALDPTSPFDANGCWSGSATILPGALPAILYTGIDASKEQVQNV AFAKNPSDPLLREWEKPAYNPVIALPADVPGDKFRDPSTAWLGRDGLWRIAVSAEVDGVASTLVYRSKDFVRWERNAAPLHASRAAGMVECPDLFPVAER GEDGLDTSANGAGGVRHVLKLSVMDTLQDYYMVGTYDDAADAFSPAEPERGDDCRSWRRLDYGHVYASKSFFDVRKNRRVLWAWANESDSQADDVARGWS GVQTFPRKMWLAKDGKQLLQWP |
Full Sequence |
---|
Protein Sequence Length: 596 Download |
MARLGLAVCA ASFHLFLLLA STSSLRRAPT EADTANHARR TAYHFQPAKN WQNDAGPMYH 60 NGMYHLFYQY NPHSALWDIG NLSWGHSVSG DLLNWAALDT ALDPTSPFDA NGCWSGSATI 120 LPGALPAILY TGIDASKEQV QNVAFAKNPS DPLLREWEKP AYNPVIALPA DVPGDKFRDP 180 STAWLGRDGL WRIAVSAEVD GVASTLVYRS KDFVRWERNA APLHASRAAG MVECPDLFPV 240 AERGEDGLDT SANGAGGVRH VLKLSVMDTL QDYYMVGTYD DAADAFSPAE PERGDDCRSW 300 RRLDYGHVYA SKSFFDVRKN RRVLWAWANE SDSQADDVAR GWSGVQTFPR KMWLAKDGKQ 360 LLQWPIEEIK TLRRKRAGLW QGTRLGAGAV QEIVGVASSQ ADVEVVFKIP SLEEAERVDD 420 PNRLLDPQKL CGEKGAAVRG GVGPFGLLVM ASGDLHEHTA VFFRVFRHHD KYKLLMCTDL 480 TKSSTRAGVY KPAYGGFVDM DIDDHKTISL RTLIDHSVVE SFGGGGRACI TARVYPEHVA 540 TSSSHLYVFN NGSDAVKVAK LEAWDLATAT VNVVVGDHHG LVAPALELEP TRTTQ* 600 |
Functional Domains Download unfiltered results here | ||||||
---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
TIGR01322 | scrB_fam | 3.0e-45 | 40 | 536 | 518 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. |
COG1621 | SacC | 4.0e-62 | 40 | 560 | 538 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] |
cd08996 | GH32_B_Fructosidase | 2.0e-83 | 50 | 368 | 326 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
pfam00251 | Glyco_hydro_32N | 3.0e-130 | 44 | 365 | 331 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
smart00640 | Glyco_32 | 3.0e-162 | 44 | 526 | 494 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0005773 | vacuole |
GO:0005975 | carbohydrate metabolic process |
GO:0008152 | metabolic process |
GO:0016787 | hydrolase activity |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
Swiss-Prot | A2YZ01 | 0 | 1 | 595 | 1 | 596 | INV7_ORYSI RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 7; AltName: Full=Sucrose hydrolase 7; AltName: Full=Invertase 7; AltName: Full=Cell wall beta-fructosidase 7; AltName: Full=OsCIN7; Flags: Precursor |
GenBank | AAD38399.1 | 0 | 1 | 590 | 1 | 591 | AF155121_1 apoplastic invertase [Oryza sativa Indica Group] |
DDBJ | BAD23559.1 | 0 | 1 | 595 | 1 | 595 | putative apoplastic invertase [Oryza sativa Japonica Group] |
GenBank | EEC84182.1 | 0 | 1 | 595 | 1 | 595 | hypothetical protein OsI_30564 [Oryza sativa Indica Group] |
RefSeq | NP_001062691.1 | 0 | 1 | 595 | 1 | 596 | Os09g0255000 [Oryza sativa (japonica cultivar-group)] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 40 | 573 | 8 | 535 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 2ade_A | 0 | 40 | 573 | 8 | 535 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 2add_A | 0 | 40 | 573 | 8 | 535 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
PDB | 1st8_A | 0 | 40 | 573 | 8 | 535 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 40 | 573 | 8 | 535 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
Metabolic Pathways | |||
---|---|---|---|
Pathway Name | Reaction | EC | Protein Name |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |