Basic Information | |
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Species | Oryza sativa |
Cazyme ID | LOC_Os09g08120.1 |
Family | GH32 |
Protein Properties | Length: 388 Molecular Weight: 42245.3 Isoelectric Point: 4.8582 |
Chromosome | Chromosome/Scaffold: 9 Start: 4190886 End: 4198627 |
Description | beta-fructofuranosidase 5 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 2 | 251 | 0 |
YHLFYKYNPHSALWDIGNLSWGHFVSGDLLNWAALDTALDPTSPFDANGCWSGSATILPGGLPAILYTGIDAGKEQVQNVAFAKNPSDPLLREWEKPAYN PVIALPPDVPGDNFRDPSTAWLGRDGLWRIAVSAEVDGVASTLVYRSEDFVRWERNAAPLHASRAAGMVECPDMFPVAENGEDGLDTSTNGAGGVRHVLK LSVMDTLQDYYMVGTYDDAADAFSPAEPERGDDCRRWRRLDYGHTFPRKM |
Full Sequence |
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Protein Sequence Length: 388 Download |
MYHLFYKYNP HSALWDIGNL SWGHFVSGDL LNWAALDTAL DPTSPFDANG CWSGSATILP 60 GGLPAILYTG IDAGKEQVQN VAFAKNPSDP LLREWEKPAY NPVIALPPDV PGDNFRDPST 120 AWLGRDGLWR IAVSAEVDGV ASTLVYRSED FVRWERNAAP LHASRAAGMV ECPDMFPVAE 180 NGEDGLDTST NGAGGVRHVL KLSVMDTLQD YYMVGTYDDA ADAFSPAEPE RGDDCRRWRR 240 LDYGHTFPRK MWIAKDGKQL LQWPIEEIET LRRKRAAGLR RGTRLGAGAV QEIVGVASSQ 300 ADVEVIDHSV VESFGGGGRA CITARVYPEH VATSSSHLYV FNNGSDDVKV AKLEAWDLAT 360 ATVNVVVGDH HGLAAPALEL EPTRTTQ* |
Functional Domains Download unfiltered results here | ||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description |
COG1621 | SacC | 2.0e-35 | 2 | 261 | 275 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] |
cd08996 | GH32_B_Fructosidase | 7.0e-57 | 2 | 267 | 310 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. |
smart00640 | Glyco_32 | 4.0e-87 | 2 | 269 | 314 | + Glycosyl hydrolases family 32. |
pfam00251 | Glyco_hydro_32N | 2.0e-87 | 2 | 264 | 310 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. |
Gene Ontology | |
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GO Term | Description |
GO:0005773 | vacuole |
GO:0005975 | carbohydrate metabolic process |
GO:0008152 | metabolic process |
GO:0016787 | hydrolase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAD38399.1 | 0 | 1 | 306 | 64 | 407 | AF155121_1 apoplastic invertase [Oryza sativa Indica Group] |
GenBank | AAD38399.1 | 2e-38 | 305 | 382 | 514 | 591 | AF155121_1 apoplastic invertase [Oryza sativa Indica Group] |
DDBJ | BAH94464.1 | 0 | 1 | 387 | 64 | 450 | Os09g0255266 [Oryza sativa Japonica Group] |
GenBank | EEC84182.1 | 0 | 1 | 306 | 63 | 406 | hypothetical protein OsI_30564 [Oryza sativa Indica Group] |
GenBank | EEC84183.1 | 0 | 1 | 351 | 61 | 439 | hypothetical protein OsI_30566 [Oryza sativa Indica Group] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2qqu_A | 0 | 1 | 306 | 29 | 372 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D239a) From Arabidopsis Thaliana In Complex With Sucrose |
PDB | 2qqu_A | 0.0000000000002 | 305 | 361 | 478 | 534 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D239a) From Arabidopsis Thaliana In Complex With Sucrose |
PDB | 2ac1_A | 0 | 1 | 306 | 33 | 376 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
PDB | 2ac1_A | 0.00000000000008 | 305 | 364 | 482 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
PDB | 2xqr_K | 0 | 1 | 306 | 29 | 372 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
CB670474 | 183 | 1 | 183 | 0 |
CB681398 | 168 | 1 | 168 | 0 |
FL914923 | 205 | 62 | 264 | 0 |
GR348533 | 234 | 1 | 231 | 0 |
DR801268 | 229 | 1 | 228 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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