| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os10g05069.2 |
| Family | GH38 |
| Protein Properties | Length: 874 Molecular Weight: 98034.5 Isoelectric Point: 6.9416 |
| Chromosome | Chromosome/Scaffold: 10 Start: 2479450 End: 2489779 |
| Description | Glycosyl hydrolase family 38 protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH38 | 1 | 219 | 7.00649e-45 |
| MHDEAAVHYIDMIDQTTLGHRVIKKQFNKIPRAGWQIDPFGHSAVQGYLLGAELGFDSMHFARIDYQDRAKRKGDKGLEVIWRGSRTFGSSSQIFTNAFP VHYSPPDGFGFEIFDDFVPVQDDMLLFDYNLKERVNDFVAAALKQANVTRTNHIMWTMGDDFNYQYAESWFRNMDRLINYVNKDGRVHALYSTPSIYTDA KHASNESWPLKYDDYFPYA | |||
| Full Sequence |
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| Protein Sequence Length: 874 Download |
| MHDEAAVHYI DMIDQTTLGH RVIKKQFNKI PRAGWQIDPF GHSAVQGYLL GAELGFDSMH 60 FARIDYQDRA KRKGDKGLEV IWRGSRTFGS SSQIFTNAFP VHYSPPDGFG FEIFDDFVPV 120 QDDMLLFDYN LKERVNDFVA AALKQANVTR TNHIMWTMGD DFNYQYAESW FRNMDRLINY 180 VNKDGRVHAL YSTPSIYTDA KHASNESWPL KYDDYFPYAD AKNAYWTGYF TSRPTFKRYI 240 RMISGYYLAA RQLEFLVGRS SLGLFTSSLE DPLGIAQHHD AVSGTAKQHT TDDYSKRLAI 300 GVSQVEKGVN TALSCLTSSK GTCTATKFSQ CQLLNISYCP STEEGISSAK SLVIVVYNPL 360 GWERSDFVRV PVNDANLIVK TSDGTSLESQ LVEVDIVTAR LRKLYIKAYL GITSDKPPKY 420 WLVFQASVPP LGWNTYFISK STGTGSNGMG YVSTMVSPSN DTIEIGPGPL KMSYSSKSGQ 480 LKRMFNSISA VDLPIQQSFL WYASSTGDSE DSQASGAYIF RPNRTTPTIV SGMAPLKVIH 540 GPLVDEVHQQ FSSWIYQVTR LYKNKEHAEV EYTIGPIPVD DDDDIGKEVV TRLTTNMATN 600 KIFYTDSNGR DFLERVRNHR DDWDLNLSQP VAGNYYPVNQ GIYVADGKYE LSVLVDHAVG 660 ASSIQDGQIE VMLHRRLSAD DGRGVGEPLN EVVCVDQKCD GLVARATYYI NVNKKGHGAH 720 WRRTYSQQVY SPFLVAFAHE DERSWKSNNI AKASTVEGNY SLPDNVAIIT LQSLDDGTTL 780 LRLAHLFQAQ EDTQYSVMAK VELRKLFGKR IIKDLTETSL SANQKKSEMK KLNWRVTGES 840 KTDPAPLKGG PVDSHALVVE LGPMEIRTFL LKF* 900 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam07748 | Glyco_hydro_38C | 3.0e-41 | 456 | 869 | 444 | + Glycosyl hydrolases family 38 C-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. | ||
| pfam01074 | Glyco_hydro_38 | 2.0e-56 | 1 | 219 | 223 | + Glycosyl hydrolases family 38 N-terminal domain. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. | ||
| cd00451 | GH38N_AMII_euk | 3.0e-60 | 1 | 183 | 183 | + N-terminal catalytic domain of eukaryotic class II alpha-mannosidases; glycoside hydrolase family 38 (GH38). The family corresponds to a group of eukaryotic class II alpha-mannosidases (AlphaMII), which contain Golgi alpha-mannosidases II (GMII), the major broad specificity lysosomal alpha-mannosidases (LAM, MAN2B1), the noval core-specific lysosomal alpha 1,6-mannosidases (Epman, MAN2B2), and similar proteins. GMII catalyzes the hydrolysis of the terminal both alpha-1,3-linked and alpha-1,6-linked mannoses from the high-mannose oligosaccharide GlcNAc(Man)5(GlcNAc)2 to yield GlcNAc(Man)3(GlcNAc)2 (GlcNAc, N-acetylglucosmine), which is the committed step of complex N-glycan synthesis. LAM is a broad specificity exoglycosidase hydrolyzing all known alpha 1,2-, alpha 1,3-, and alpha 1,6-mannosidic linkages from numerous high mannose type oligosaccharides. Different from LAM, Epman can efficiently cleave only the alpha 1,6-linked mannose residue from (Man)3GlcNAc, but not (Man)3(GlcNAc)2 or other larger high mannose oligosaccharides, in the core of N-linked glycans. Members in this family are retaining glycosyl hydrolases of family GH38 that employs a two-step mechanism involving the formation of a covalent glycosyl enzyme complex. Two carboxylic acids positioned within the active site act in concert: one as a catalytic nucleophile and the other as a general acid/base catalyst. | ||
| PLN02701 | PLN02701 | 2.0e-76 | 1 | 692 | 788 | + alpha-mannosidase | ||
| cd10810 | GH38N_AMII_LAM_like | 9.0e-104 | 1 | 183 | 185 | + N-terminal catalytic domain of lysosomal alpha-mannosidase and similar proteins; glycoside hydrolase family 38 (GH38). The subfamily is represented by lysosomal alpha-mannosidase (LAM, Man2B1, EC 3.2.1.114), which is a broad specificity exoglycosidase hydrolyzing all known alpha 1,2-, alpha 1,3-, and alpha 1,6-mannosidic linkages from numerous high mannose type oligosaccharides. LAM is expressed in all tissues and in many species. In mammals, the absence of LAM can cause the autosomal recessive disease alpha-mannosidosis. LAM has an acidic pH optimum at 4.0-4.5. It is stimulated by zinc ion and is inhibited by cobalt ion and plant alkaloids, such as swainsonine (SW). LAM catalyzes hydrolysis by a double displacement mechanism in which a glycosyl-enzyme intermediate is formed and hydrolyzed via oxacarbenium ion-like transition states. A carboxylic acid in the active site acts as the catalytic nucleophile in the formation of the covalent intermediate while a second carboxylic acid acts as a general acid catalyst. The same residue is thought to assist in the hydrolysis (deglycosylation) step, this time acting as a general base. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0004559 | alpha-mannosidase activity |
| GO:0005488 | binding |
| GO:0005618 | cell wall |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ABG65905.1 | 0 | 59 | 873 | 1 | 815 | glycosyl hydrolase family 38 protein, putative, expressed [Oryza sativa (japonica cultivar-group)] |
| GenBank | ABG65906.1 | 0 | 1 | 873 | 1 | 873 | glycosyl hydrolase family 38 protein, putative, expressed [Oryza sativa (japonica cultivar-group)] |
| GenBank | EEC66555.1 | 0 | 1 | 873 | 132 | 1004 | hypothetical protein OsI_32713 [Oryza sativa Indica Group] |
| RefSeq | NP_001064140.1 | 0 | 1 | 873 | 132 | 1004 | Os10g0140200 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002464752.1 | 0 | 1 | 873 | 134 | 1007 | hypothetical protein SORBIDRAFT_01g026390 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1o7d_D | 0 | 470 | 735 | 10 | 268 | C Chain C, The Structure Of The Bovine Lysosomal A-Mannosidase Suggests A Novel Mechanism For Low Ph Activation |
| PDB | 1ps3_A | 0 | 1 | 687 | 167 | 880 | A Chain A, Golgi Alpha-mannosidase Ii In Complex With Kifunensine |
| PDB | 1hxk_A | 0 | 1 | 687 | 137 | 850 | A Chain A, Golgi Alpha-Mannosidase Ii In Complex With Deoxymannojirimicin |
| PDB | 1hww_A | 0 | 1 | 687 | 137 | 850 | A Chain A, Golgi Alpha-Mannosidase Ii In Complex With Deoxymannojirimicin |
| PDB | 1hty_A | 0 | 1 | 687 | 137 | 850 | A Chain A, Golgi Alpha-Mannosidase Ii |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO780062 | 604 | 200 | 797 | 0 |
| HO780062 | 81 | 120 | 200 | 0 |
| CB647849 | 272 | 33 | 304 | 0 |
| CB627339 | 279 | 264 | 542 | 0 |
| HO780062 | 23 | 279 | 301 | 0.73 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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