| Basic Information | |
|---|---|
| Species | Oryza sativa |
| Cazyme ID | LOC_Os10g34230.1 |
| Family | AA7 |
| Protein Properties | Length: 528 Molecular Weight: 58428.7 Isoelectric Point: 6.6549 |
| Chromosome | Chromosome/Scaffold: 10 Start: 18270169 End: 18274977 |
| Description | cytokinin oxidase/dehydrogenase 1 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| AA7 | 54 | 245 | 6.9e-24 |
| HSPSAVLKPQAPRDISLLLSFLSASPLGKVTVAARGAGHSIHGQAQALDGIVVEMSSLPSEIEFYRRGEGDVSYADVGGGIMWIELLEQSLKLGLAPRSW TDYLYLTIGGTLSNAGISGQTFKHGPQISNVLQLEVVTGRGEIVTCSPTKDAELFNAVLGGLGQFGIITRARILLQEAPQKVKWVRAFYDDF | |||
| Full Sequence |
|---|
| Protein Sequence Length: 528 Download |
| MEVAMVCTRV NLLILILSLC SPYKFIQSPM DFGPLNLLPT TTTASSDFGR ILFHSPSAVL 60 KPQAPRDISL LLSFLSASPL GKVTVAARGA GHSIHGQAQA LDGIVVEMSS LPSEIEFYRR 120 GEGDVSYADV GGGIMWIELL EQSLKLGLAP RSWTDYLYLT IGGTLSNAGI SGQTFKHGPQ 180 ISNVLQLEVV TGRGEIVTCS PTKDAELFNA VLGGLGQFGI ITRARILLQE APQKVKWVRA 240 FYDDFATFTK DQELLVSMPV LVDYVEGFIV LNEQSLHSSS IAFPTNVDFN PDFGTKNNPK 300 IYYCIEFAVH DYQNKNINVE QVVEVISRQM SHIASHLYSV EVSYFDFLNR VRMEEMSLRN 360 SGLWEVHHPW LNMFVPSAGI SDFRDLLMDS ISPDNFEGLI LIYPLLRHKW DTNTSVVLPD 420 SGSTDQVMYA VGILRSANPD DGCSHHCLQE LLLRHRRLAG AAASGLGAKQ YLAHHPTPAG 480 WRRHFGRRWE RFADRKARFD PRCILGPGQG IFPRDSSSSN GAFASYS* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01678 | FAD_lactone_ox | 4.0e-8 | 89 | 226 | 140 | + sugar 1,4-lactone oxidases. This model represents a family of at least two different sugar 1,4 lactone oxidases, both involved in synthesizing ascorbic acid or a derivative. These include L-gulonolactone oxidase (EC 1.1.3.8) from rat and D-arabinono-1,4-lactone oxidase (EC 1.1.3.37) from Saccharomyces cerevisiae. Members are proposed to have the cofactor FAD covalently bound at a site specified by Prosite motif PS00862; OX2_COVAL_FAD; 1. | ||
| pfam01565 | FAD_binding_4 | 3.0e-21 | 56 | 200 | 146 | + FAD binding domain. This family consists of various enzymes that use FAD as a co-factor, most of the enzymes are similar to oxygen oxidoreductase. One of the enzymes Vanillyl-alcohol oxidase (VAO) has a solved structure, the alignment includes the FAD binding site, called the PP-loop, between residues 99-110. The FAD molecule is covalently bound in the known structure, however the residue that links to the FAD is not in the alignment. VAO catalyzes the oxidation of a wide variety of substrates, ranging form aromatic amines to 4-alkylphenols. Other members of this family include D-lactate dehydrogenase, this enzyme catalyzes the conversion of D-lactate to pyruvate using FAD as a co-factor; mitomycin radical oxidase, this enzyme oxidises the reduced form of mitomycins and is involved in mitomycin resistance. This family includes MurB an UDP-N-acetylenolpyruvoylglucosamine reductase enzyme EC:1.1.1.158. This enzyme is involved in the biosynthesis of peptidoglycan. | ||
| COG0277 | GlcD | 2.0e-22 | 56 | 511 | 475 | + FAD/FMN-containing dehydrogenases [Energy production and conversion] | ||
| pfam09265 | Cytokin-bind | 2.0e-134 | 232 | 512 | 288 | + Cytokinin dehydrogenase 1, FAD and cytokinin binding. Members of this family adopt an alpha+beta sandwich structure with an antiparallel beta-sheet, in a ferredoxin-like fold. They are predominantly found in plant cytokinin dehydrogenase 1, where they are capable of binding both FAD and cytokinin substrates. The substrate displays a 'plug-into-socket' binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. | ||
| PLN02441 | PLN02441 | 0 | 1 | 515 | 534 | + cytokinin dehydrogenase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0008152 | metabolic process |
| GO:0008762 | UDP-N-acetylmuramate dehydrogenase activity |
| GO:0009056 | catabolic process |
| GO:0009653 | anatomical structure morphogenesis |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CAE05712.2 | 0 | 14 | 520 | 12 | 531 | OSJNBb0065J09.8 [Oryza sativa (japonica cultivar-group)] |
| GenBank | EAY82296.1 | 0 | 1 | 527 | 1 | 527 | hypothetical protein OsI_37507 [Oryza sativa Indica Group] |
| GenBank | EAZ19670.1 | 0 | 1 | 409 | 1 | 409 | hypothetical protein OsJ_35246 [Oryza sativa Japonica Group] |
| RefSeq | NP_001064886.1 | 0 | 1 | 527 | 1 | 527 | Os10g0483500 [Oryza sativa (japonica cultivar-group)] |
| RefSeq | XP_002467062.1 | 0 | 1 | 514 | 1 | 514 | hypothetical protein SORBIDRAFT_01g019000 [Sorghum bicolor] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2q4w_A | 0 | 44 | 514 | 50 | 522 | A Chain A, Crystal Structure Of Medicago Truncatula Ugt71g1 Complexed With Udp-Glucose |
| PDB | 2exr_A | 0 | 44 | 514 | 50 | 522 | A Chain A, X-Ray Structure Of Cytokinin OxidaseDEHYDROGENASE (CKX) FROM Arabidopsis Thaliana At5g21482 |
| PDB | 3s1e_A | 0 | 41 | 512 | 36 | 515 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| PDB | 3s1c_A | 0 | 41 | 512 | 36 | 515 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
| PDB | 3dq0_A | 0 | 41 | 512 | 36 | 515 | A Chain A, Pro427gln Mutant Of Maize Cytokinin OxidaseDEHYDROGENASE COMPLEXED With N6-Isopentenyladenine |
