y
Basic Information | |
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Species | Linum usitatissimum |
Cazyme ID | Lus10004031 |
Family | GH32 |
Protein Properties | Length: 661 Molecular Weight: 74012.3 Isoelectric Point: 5.1242 |
Chromosome | Chromosome/Scaffold: 1155 Start: 177319 End: 179612 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 112 | 430 | 0 |
HFQSEKNWMNDPNGPLYYKGWYHFFYQYNPFSAVWGDIAWGHAVSKDLVRWVHLPLAMVPDQWYDVNGVWTGSATILPDGKIVMLYTGSTNDSVQVQCLA YPANHDDPLLLDWVKYSGNPVLVPPPGIGIKDFRDPTTAWMTSEGKWRITVGSKIHRTGIALVYDTEDFIHYEQKNETLHGVSGTGMWECVDFYPVSMTG SDGLDTSTNGPDVKHVMKASLDDDRHDYYALGIYDDQTGKWTPDNSEIDVGIGIRYDYGVFYASKTFYDESKKRRVLWGWVGESDSEFSDVKKGWACLQG FPRTVSMDIKTKTNLIQWP |
Full Sequence |
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Protein Sequence Length: 661 Download |
MADQRTPILP VADPDYIPIP DDSTSPGRFS TRNRSKKLLL FSLFCGVALV WLIMGNGSDH 60 SVDIAHTPST ATKTTSRGKL EGVSEKSNGQ RSLFRDHDWE NKTLSWQRTA FHFQSEKNWM 120 NDPNGPLYYK GWYHFFYQYN PFSAVWGDIA WGHAVSKDLV RWVHLPLAMV PDQWYDVNGV 180 WTGSATILPD GKIVMLYTGS TNDSVQVQCL AYPANHDDPL LLDWVKYSGN PVLVPPPGIG 240 IKDFRDPTTA WMTSEGKWRI TVGSKIHRTG IALVYDTEDF IHYEQKNETL HGVSGTGMWE 300 CVDFYPVSMT GSDGLDTSTN GPDVKHVMKA SLDDDRHDYY ALGIYDDQTG KWTPDNSEID 360 VGIGIRYDYG VFYASKTFYD ESKKRRVLWG WVGESDSEFS DVKKGWACLQ GFPRTVSMDI 420 KTKTNLIQWP VEEIETLRRG KREFNEIGLN PGSILQLGID DGSQLDIVAE FELDKEAVNW 480 SSTAELADFN CADSKGAAQR GALGPFGLLV LTEETLTEQT PVYFYVTKGR NETLTTFFCV 540 DQSRSSLATD VSKVIHGSYV PTLEGEKISV RVLVDHSIVE SFVQGGRSVI TARVYPTMAI 600 YGSAKVFLFN NATEAAVKAS VQVWEMGSAY EPVSDSVRSG PRLGFVVFFY MLCYLALKFY 660 * 720 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 5.0e-57 | 108 | 596 | 504 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 8.0e-75 | 108 | 597 | 507 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-96 | 118 | 433 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 1.0e-147 | 112 | 430 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 2.0e-171 | 112 | 586 | 485 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAB82419.1 | 0 | 82 | 630 | 92 | 640 | acid invertase [Citrus unshiu] |
DDBJ | BAF34362.1 | 0 | 82 | 630 | 92 | 640 | beta-fructofuranosidase [Citrus sinensis] |
DDBJ | BAF35859.1 | 0 | 1 | 657 | 1 | 679 | soluble acid invertase [Pyrus pyrifolia var. culta] |
RefSeq | XP_002303519.1 | 0 | 1 | 630 | 1 | 653 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002532576.1 | 0 | 73 | 630 | 83 | 641 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 97 | 630 | 8 | 540 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
PDB | 3ugh_A | 0 | 97 | 630 | 8 | 540 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
PDB | 3ugg_B | 0 | 97 | 630 | 8 | 540 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
PDB | 3ugg_A | 0 | 97 | 630 | 8 | 540 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
PDB | 3ugf_B | 0 | 97 | 630 | 8 | 540 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |