| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10004340 |
| Family | GH32 |
| Protein Properties | Length: 645 Molecular Weight: 71266.4 Isoelectric Point: 6.2904 |
| Chromosome | Chromosome/Scaffold: 1134 Start: 121907 End: 124349 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
|---|
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 125 | 440 | 0 |
| HFQPEKNWMNGPLFYKGWYHFFYQYNPNAPVWGDIVWGHAVSRDLIHWLHLPLAMVADQWYDVNGVWTGSATILPNGNLVMLYTGSTNESVQVQNLAYPA DHNDPLLLNWVKYPGNPVLFPPQGIHFKDFRDPTTAWRTPEGKWRITIGSKVNKTGIALVYDTDDFINFDLKDGILHGVPGTGMWECVDFYPVAVDGGNG MDTSANGAGVKHVMKTSLDDDRHDYYAIGSYDEVNAKWVPDNPEIDVGIGIRYDYGIFYASKTFYDQSKQRRVLWGWIGESDSEAADVRKGWASLQGIPR TVVLDTKTGSNLLQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 645 Download |
| MADNKQPFLP VTGAEPSKNH APSSGSRKKL LLAAFGGIFV VCLILAGGGY DKSGLVKSAR 60 FGGGGSDLDE PAGNAIRSAV SRGKSEGVSE KANLPEHHLL RGENAVTEEF PWNNSMLSWQ 120 RTAFHFQPEK NWMNGPLFYK GWYHFFYQYN PNAPVWGDIV WGHAVSRDLI HWLHLPLAMV 180 ADQWYDVNGV WTGSATILPN GNLVMLYTGS TNESVQVQNL AYPADHNDPL LLNWVKYPGN 240 PVLFPPQGIH FKDFRDPTTA WRTPEGKWRI TIGSKVNKTG IALVYDTDDF INFDLKDGIL 300 HGVPGTGMWE CVDFYPVAVD GGNGMDTSAN GAGVKHVMKT SLDDDRHDYY AIGSYDEVNA 360 KWVPDNPEID VGIGIRYDYG IFYASKTFYD QSKQRRVLWG WIGESDSEAA DVRKGWASLQ 420 GIPRTVVLDT KTGSNLLQWP VEEAEKLRLR SQEFKNVVAK PGSVVPIEVE SATQLDVVAE 480 FELDKEAVEK AVESDEEYKC SKGAAQRGAL GPFGLLVLSE DTLSEQTPVY FYVVKGKNGT 540 LRTSFCTDQS RSSLANDVNK KLYGSYVPVL EGEKFKVRML VDHSIIESFA QGGRSVITSR 600 VYPTKAIYGA ARLFLFNNAT GAGVTASLKV WQMNSAFIRP YPNL* 660 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 3.0e-52 | 121 | 603 | 509 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 1.0e-71 | 121 | 632 | 529 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 2.0e-92 | 131 | 443 | 322 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 2.0e-150 | 125 | 440 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 0 | 125 | 593 | 482 | + Glycosyl hydrolases family 32. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004564 | beta-fructofuranosidase activity |
| GO:0004575 | sucrose alpha-glucosidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| DDBJ | BAB82419.1 | 0 | 81 | 643 | 86 | 646 | acid invertase [Citrus unshiu] |
| DDBJ | BAF34362.1 | 0 | 81 | 643 | 86 | 646 | beta-fructofuranosidase [Citrus sinensis] |
| Swiss-Prot | P29001 | 0 | 3 | 642 | 2 | 642 | INVA_PHAAU RecName: Full=Acid beta-fructofuranosidase; AltName: Full=Acid sucrose hydrolase; AltName: Full=Acid invertase; Short=AI; AltName: Full=Vacuolar invertase; Contains: RecName: Full=Acid beta-fructofuranosidase 30 kDa subunit; Contains: RecName: Full=Acid beta-fructofuranosidase 38 kDa subunit; Flags: Precursor |
| RefSeq | XP_002303519.1 | 0 | 81 | 643 | 98 | 659 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002532576.1 | 0 | 76 | 644 | 81 | 648 | Acid beta-fructofuranosidase precursor, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3ugh_B | 0 | 103 | 641 | 3 | 544 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
| PDB | 3ugh_A | 0 | 103 | 641 | 3 | 544 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
| PDB | 3ugg_B | 0 | 103 | 641 | 3 | 544 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
| PDB | 3ugg_A | 0 | 103 | 641 | 3 | 544 | A Chain A, 3.0 A Structure Of The Prunus Mume Hydroxynitrile Lyase Isozyme-1 |
| PDB | 3ugf_B | 0 | 103 | 641 | 3 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
