| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10007526 |
| Family | GH85 |
| Protein Properties | Length: 736 Molecular Weight: 83739.4 Isoelectric Point: 5.8844 |
| Chromosome | Chromosome/Scaffold: 1519 Start: 265972 End: 269243 |
| Description | Glycosyl hydrolase family 85 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH85 | 148 | 429 | 0 |
| MQGGYVDDKWVQGGSNPDAYSIWHWYLMDVCVYFSHSLVTLPPPCWTNVAHRHGVKVLGTFITEWDEGRTISNKLLETEDSARLYAERLAELAASLGFDG WLINMEVKLDPSQIPNLKEFVRHLTWTMHSLVPGSMVIWYDSVTIDGELKWQDQLNGKNKAFFDICDGIFVNYTWKKDYPKLSAEVAGDRKFDVYMGIDV FGRNTYGGGMWDTNVALDVIKSNEVSAAIFAPGWVYETKQPPDFRTAQNRWWNLVENSWGVVQKYPRSLPFYSSFDQGVGYH | |||
| Full Sequence |
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| Protein Sequence Length: 736 Download |
| MNGCSSSLRW AHCKIAEWWC LFKQVKVSSL FKSINQSPEY MLARLLRLVI SRETLISVYN 60 LLKFLRTITK SILSRIFSMA KSEQQQQDAE PAFDPLQPSI PISYPLKTLE ELESRAYFDS 120 FHYPFNKSSV PLESPLPNRP RMLVCHDMQG GYVDDKWVQG GSNPDAYSIW HWYLMDVCVY 180 FSHSLVTLPP PCWTNVAHRH GVKVLGTFIT EWDEGRTISN KLLETEDSAR LYAERLAELA 240 ASLGFDGWLI NMEVKLDPSQ IPNLKEFVRH LTWTMHSLVP GSMVIWYDSV TIDGELKWQD 300 QLNGKNKAFF DICDGIFVNY TWKKDYPKLS AEVAGDRKFD VYMGIDVFGR NTYGGGMWDT 360 NVALDVIKSN EVSAAIFAPG WVYETKQPPD FRTAQNRWWN LVENSWGVVQ KYPRSLPFYS 420 SFDQGVGYHI SVDGEQITDA TWNNMSCQGF QPLLTYADDS KPDAIQVLVE EPSYNGGGNV 480 TFKGTLQDNA YFETRLFLGE HLLGCEPLQV LYSVKSEKDD SQLGLSLHFV SPLNNKESVI 540 LIAPWETEEP SRKFNKAIVP RLVTDSETQT GRWMVYKSKV VMNGHILREI RAVCHRLNGG 600 RPEYSAVLGH ILMKSSDQNM NLPPANLWEV TAEFVRWSTD VSSAVSLKIK WKLKEGITTF 660 RIPTYNVYYV AKQAEQRDGD SNYLGVAAVE AFYVLDLAVR SDVCSLKFIV QACSADGASL 720 KLDDCPYFEL EVEHK* 780 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam14103 | DUF4276 | 0.0007 | 548 | 608 | 62 | + Domain of unknown function (DUF4276). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria and archaea. Proteins in this family are typically between 190 and 224 amino acids in length. There is a single completely conserved residue E that may be functionally important. | ||
| COG4724 | COG4724 | 1.0e-33 | 125 | 497 | 415 | + Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism] | ||
| pfam03644 | Glyco_hydro_85 | 9.0e-114 | 148 | 432 | 304 | + Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates. | ||
| cd06547 | GH85_ENGase | 4.0e-127 | 143 | 453 | 331 | + Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0005737 | cytoplasm |
| GO:0033925 | mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | XP_002273683.1 | 0 | 79 | 733 | 1 | 688 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002315137.1 | 0 | 99 | 733 | 26 | 697 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002315138.1 | 0 | 88 | 733 | 16 | 694 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002520781.1 | 0 | 79 | 733 | 1 | 691 | endo beta n-acetylglucosaminidase, putative [Ricinus communis] |
| RefSeq | XP_002520784.1 | 0 | 78 | 733 | 2 | 685 | endo beta n-acetylglucosaminidase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 3fha_D | 1e-27 | 124 | 527 | 28 | 464 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fha_C | 1e-27 | 124 | 527 | 28 | 464 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fha_B | 1e-27 | 124 | 527 | 28 | 464 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fha_A | 1e-27 | 124 | 527 | 28 | 464 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| PDB | 3fhq_F | 1e-27 | 124 | 527 | 28 | 464 | A Chain A, Structure Of Endo-Beta-N-Acetylglucosaminidase A |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| JG074607 | 303 | 436 | 736 | 0 |
| EL437937 | 257 | 167 | 423 | 0 |
| JG621224 | 277 | 192 | 467 | 0 |
| GR107739 | 245 | 142 | 386 | 0 |
| EL452818 | 290 | 65 | 348 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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