y
Basic Information | |
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Species | Linum usitatissimum |
Cazyme ID | Lus10008067 |
Family | GH31 |
Protein Properties | Length: 1053 Molecular Weight: 116108 Isoelectric Point: 6.4093 |
Chromosome | Chromosome/Scaffold: 374 Start: 101435 End: 105776 |
Description | Glycosyl hydrolases family 31 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH31 | 425 | 893 | 0 |
YIFAGESPVDVIEQYTEFIGRPAPMPYWSFGFHQCKYGYKNLSEVEAVVAGYAKAGIPLEVMWTDIDYMDAYKDFTLDPVTFPVMQMRNFVNRLHQFGQK FVPILDPGISVNDSYGTYIRGVEADIFIKRDGAPYLGEVWPGKVYFPDFLNPNASDFWREEIQRFLYILPIDGLWIDMNELSNFITSTPTPNSTLDHPPY MINNGGNHRPIVNKTTPATCLHSGNVTEYNVHNMYGLLQARATNDALIRTTGKRPFVLSRSTFVSSGKYAAHWTGDIASTWEDLANTIPSILNSGLFGIP MVGADICGFLLNTTEELCLRWIQLGAFYPFARDHSDKNTIRQELYVWESVAEAARKALGLRYKLLPYLYTLMAEANQRGTPIARPLFFSFPEDTNTYKIN SQFLLGSGVMITPVVKQGAVSVKGYFPAGVWFDLFDHSNGTIVVSSGNYVTLEASPDHINVHLKDGYIV |
Full Sequence |
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Protein Sequence Length: 1053 Download |
MSVNKETKTF VVGTVGTTPI TASITAKFSH TPANVFFVIA NAMASNHNFM MIGSELLGLD 60 YKGLKLPLVP VLDMLNAVLL SAGVNSAGFM AQLAKNGNSH ARWNEICDNF EGYCNRGGIA 120 ILGSYVGMLI TLLITVISIV KLITMERRNK PSSNPLLPFL IFSCSFLLHI PLSYVSAAAQ 180 QVVGYGYSLG DVSVDLVTNS LTADLNLIQT SSLYGPDIPT LIFSACFDTE KRLRIRISDP 240 ETARWEIPEP IIPRRPRGHG VMLQEDTGVF SHPRSDLVIA IQNTEPFGFT VSRKSTGDVL 300 FDSSPEGSDS STYLVFKDQY IQLSSKLPGN RSSIYGLGEH TKPNFKLTPN QTLTLWNVDQ 360 ASSTLDVNLY GSHPFYIDVR SPSEDGKVAA GTTHGVLLLN SNGMDVVYTG DRITYKAIGG 420 IIDLYIFAGE SPVDVIEQYT EFIGRPAPMP YWSFGFHQCK YGYKNLSEVE AVVAGYAKAG 480 IPLEVMWTDI DYMDAYKDFT LDPVTFPVMQ MRNFVNRLHQ FGQKFVPILD PGISVNDSYG 540 TYIRGVEADI FIKRDGAPYL GEVWPGKVYF PDFLNPNASD FWREEIQRFL YILPIDGLWI 600 DMNELSNFIT STPTPNSTLD HPPYMINNGG NHRPIVNKTT PATCLHSGNV TEYNVHNMYG 660 LLQARATNDA LIRTTGKRPF VLSRSTFVSS GKYAAHWTGD IASTWEDLAN TIPSILNSGL 720 FGIPMVGADI CGFLLNTTEE LCLRWIQLGA FYPFARDHSD KNTIRQELYV WESVAEAARK 780 ALGLRYKLLP YLYTLMAEAN QRGTPIARPL FFSFPEDTNT YKINSQFLLG SGVMITPVVK 840 QGAVSVKGYF PAGVWFDLFD HSNGTIVVSS GNYVTLEASP DHINVHLKDG YIVPMQGEGM 900 TTREARATPF HLLVAVSSNG SSSSSSRGEV YLDDGERVEI GEEGGDWMKV RFEGGAIGNR 960 AYVNSEVING RRFGSRNSGW IIKRVSFLGL NKDVHQVGRE YDVSISKGSN LRGSNSVVVK 1020 VKPICHSNLV TVEISQLSLI VAQGFSLDLK LI* 1080 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06600 | GH31_MGAM-like | 4.0e-106 | 444 | 803 | 363 | + This family includes the following closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), lysosomal acid alpha-glucosidase (GAA), neutral alpha-glucosidase C (GANC), the alpha subunit of neutral alpha-glucosidase AB (GANAB), and alpha-glucosidase II. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. Alpha-glucosidase II is a GH31 enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. The MGAM-like family corresponds to subgroup 1 in the Ernst et al classification of GH31 enzymes. | ||
cd06604 | GH31_glucosidase_II_MalA | 1.0e-128 | 444 | 803 | 363 | + Alpha-glucosidase II (alpha-D-glucoside glucohydrolase) is a glycosyl hydrolase family 31 (GH31) enzyme, found in bacteria and plants, which has exo-alpha-1,4-glucosidase and oligo-1,6-glucosidase activities. Alpha-glucosidase II has been characterized in Bacillus thermoamyloliquefaciens where it forms a homohexamer. This family also includes the MalA alpha-glucosidase from Sulfolobus sulfataricus and the AglA alpha-glucosidase from Picrophilus torridus. MalA is part of the carbohydrate-metabolizing machinery that allows this organism to utilize carbohydrates, such as maltose, as the sole carbon and energy source. | ||
COG1501 | COG1501 | 4.0e-142 | 264 | 957 | 709 | + Alpha-glucosidases, family 31 of glycosyl hydrolases [Carbohydrate transport and metabolism] | ||
pfam01055 | Glyco_hydro_31 | 0 | 425 | 893 | 472 | + Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases. | ||
cd06602 | GH31_MGAM_SI_GAA | 0 | 444 | 821 | 383 | + This family includes the following three closely related glycosyl hydrolase family 31 (GH31) enzymes: maltase-glucoamylase (MGAM), sucrase-isomaltase (SI), and lysosomal acid alpha-glucosidase (GAA), also known as acid-maltase. MGAM is one of the two enzymes responsible for catalyzing the last glucose-releasing step in starch digestion. SI is implicated in the digestion of dietary starch and major disaccharides such as sucrose and isomaltose, while GAA degrades glycogen in the lysosome, cleaving both alpha-1,4 and alpha-1,6 glucosidic linkages. MGAM and SI are anchored to small-intestinal brush-border epithelial cells. The absence of SI from the brush border membrane or its malfunction is associated with malabsorption disorders such as congenital sucrase-isomaltase deficiency (CSID). The domain architectures of MGAM and SI include two tandem GH31 catalytic domains, an N-terminal domain found near the membrane-bound end, and a C-terminal luminal domain. Both of the tandem GH31 domains of MGAM and SI are included in this family. The domain architecture of GAA includes an N-terminal TFF (trefoil factor family) domain in addition to the GH31 catalytic domain. Deficient GAA expression causes pompe disease, an autosomal recessive genetic disorder also known as glycogen storage disease type II (GSDII). |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
RefSeq | XP_002298949.1 | 0 | 149 | 996 | 17 | 862 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002317679.1 | 0 | 177 | 1051 | 38 | 904 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002333838.1 | 0 | 156 | 1051 | 9 | 895 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002529880.1 | 0 | 161 | 1051 | 3 | 893 | alpha-glucosidase, putative [Ricinus communis] |
RefSeq | XP_002534242.1 | 0 | 181 | 1052 | 39 | 913 | alpha-glucosidase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3w38_A | 0 | 181 | 1051 | 39 | 908 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 3w37_A | 0 | 181 | 1051 | 39 | 908 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 3l4z_A | 0 | 201 | 936 | 65 | 774 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 3l4y_A | 0 | 201 | 936 | 65 | 774 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |
PDB | 3l4x_A | 0 | 201 | 936 | 65 | 774 | A Chain A, Heme Ligand Mutant Of Recombinant Horseradish Peroxidase In Complex With Benzhydroxamic Acid |