| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10008392 |
| Family | GH32 |
| Protein Properties | Length: 591 Molecular Weight: 65613.7 Isoelectric Point: 9.9372 |
| Chromosome | Chromosome/Scaffold: 170 Start: 179116 End: 182338 |
| Description | Glycosyl hydrolases family 32 protein |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 66 | 384 | 0 |
| HFQPPKNWINGPMIYKGIYHLFYQYNPKGAVWGNIVWAHSTSTDLVNWKPQGYGIYPSIPSDINGTWSGSATFLLDGTPAILYTGINPKNQQVQNLAVPK NASDPYLRKWVKSTKNPLMAPTPQNGINASSFRDPTTAWLGPDKTWRVIIGSKIDRKGMAILYKSKDFVNWVRANHPLHSTEGTGMWECPDFFPVLMGGY KSGIDASKLGPSVKHVLKVSLDDTKHDHYTIGKYDPIKDVYTPDHDGLAEKDFGLRYDYGKYYASKTFFDSSLNRRILLGWINESSSVADDIKKGWSGVH TIPRTIWLHRSGKQLVQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 591 Download |
| MAVLVQQHNS LLYFFALLSI SQLAFQLQAS HHVHTNFQAQ LSALSSSSSS SSSSSSKGQP 60 YRTGYHFQPP KNWINGPMIY KGIYHLFYQY NPKGAVWGNI VWAHSTSTDL VNWKPQGYGI 120 YPSIPSDING TWSGSATFLL DGTPAILYTG INPKNQQVQN LAVPKNASDP YLRKWVKSTK 180 NPLMAPTPQN GINASSFRDP TTAWLGPDKT WRVIIGSKID RKGMAILYKS KDFVNWVRAN 240 HPLHSTEGTG MWECPDFFPV LMGGYKSGID ASKLGPSVKH VLKVSLDDTK HDHYTIGKYD 300 PIKDVYTPDH DGLAEKDFGL RYDYGKYYAS KTFFDSSLNR RILLGWINES SSVADDIKKG 360 WSGVHTIPRT IWLHRSGKQL VQWPVKEIEK LRGKAVKVPA QVVNPGSRLE VSGITAAQAD 420 VEIFFGKLDL SKAERLDPSW TNLNPQLICS QRGASVRGGL GPFGFLVLGS KGLEEYTAVF 480 FRIFKVNTGK HVVLMCSDQS RSSLNNANDK TTYGTFVDID PAREYLSLRT LIDHSIVESF 540 GGFGKSCITA RVYPTIAIDG AAHLYVFNNG TQHVRIGRLN AWNMKKAHIA * 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 5.0e-47 | 60 | 398 | 366 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-61 | 59 | 555 | 518 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 4.0e-82 | 72 | 387 | 331 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 1.0e-140 | 66 | 384 | 330 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 5.0e-170 | 66 | 542 | 490 | + Glycosyl hydrolases family 32. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAT09980.1 | 0 | 32 | 589 | 19 | 575 | cell wall apoplastic invertase [Vitis vinifera] |
| GenBank | ABD28503.1 | 0 | 27 | 589 | 23 | 571 | Sialidase [Medicago truncatula] |
| DDBJ | BAB01930.1 | 0 | 20 | 590 | 19 | 581 | beta-fructofuranosidase (EC 3.2.1.26) [Arabidopsis thaliana] |
| EMBL | CBI35961.1 | 0 | 27 | 589 | 24 | 573 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002279788.1 | 0 | 27 | 589 | 24 | 574 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 54 | 590 | 1 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2qqw_A | 0 | 59 | 590 | 2 | 537 | A Chain A, Crystal Structure Of A Cell-Wall Invertase (D23a) From Arabidopsis Thaliana In Complex With Sucrose |
| PDB | 2xqr_K | 0 | 59 | 590 | 2 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 59 | 590 | 2 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 59 | 590 | 2 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
