| Basic Information | |
|---|---|
| Species | Linum usitatissimum |
| Cazyme ID | Lus10013270 |
| Family | CBM45 |
| Protein Properties | Length: 905 Molecular Weight: 101941 Isoelectric Point: 6.9512 |
| Chromosome | Chromosome/Scaffold: 372 Start: 365663 End: 370568 |
| Description | alpha-amylase-like 3 |
| View CDS | |
| External Links |
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| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| CBM45 | 318 | 394 | 5.9e-24 |
| LHWGVCRDDAKNWEIPSGPHPPETIIFKERALRTKLQAKDDGRGCSGLFTLDEDIAGFLFVLKLNDNTWFKCMGNDF | |||
| CBM45 | 136 | 219 | 6.4e-27 |
| LHWGVSYVNDSGSEWDQPPKHMRPPGSVPVKNYAIDTPLKKPSVDDLFYEVKIDIDPKSAIASINFVLKDEETGSWYQHKGRDF | |||
| GH13 | 535 | 823 | 1.9e-37 |
| EKAEDIASLGFTVIWLPPPTESVSPEGYMPKDLYNLNSRYGRIDELKDLVGRLHDSGIKVLGDAVLNHRCAHYQNKNGIWNIFGGRLSWDDRAVVADDPH FQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWMGWLREEIGYDGWRLDFVRGFWGGYVKDYLDATNPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIIDW INATNGSAGAFDVTSKGVLHAALEKCEYWRLADEKGKPPGVVGWWPSRAVTFVENHDTGSTQGHWRFPGGKEMQGYAYILTHPGTPAVF | |||
| Full Sequence |
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| Protein Sequence Length: 905 Download |
| MFSITTVEPI IRCNNRREIH GSLKATKFCS SSTSSSLNCL RQKRLVRAKF EECKLPVRRV 60 KAPAVIRASR SSSSSSSGTA LPETFESSGD VFFRQSFPVT RTETVDGKIF VRVDQSNAKG 120 DWQLTVGCNL PGKWILHWGV SYVNDSGSEW DQPPKHMRPP GSVPVKNYAI DTPLKKPSVD 180 DLFYEVKIDI DPKSAIASIN FVLKDEETGS WYQHKGRDFK VPLVDYLLDD GNFFGSNSFN 240 IWPGPFFTNI LLKSERQNNV SELNESRTED RQLEGIYEEQ PFTKHTTLEN SVTVLVRKCP 300 ETSKNLLYLE TDLSGVVLHW GVCRDDAKNW EIPSGPHPPE TIIFKERALR TKLQAKDDGR 360 GCSGLFTLDE DIAGFLFVLK LNDNTWFKCM GNDFYIPLSS SSRLLVQPGQ KHSEDAPASG 420 KAPDADQGSG TAFTDGIIKE IRNLVSGISS ENSRKVKTKE AQESILQEIE KLAAEAYSIF 480 RSSIPTFKEE VVLEEELEKA PPKKCSGTGS GYEILLQGFN WESHKSGRWY MDLKEKAEDI 540 ASLGFTVIWL PPPTESVSPE GYMPKDLYNL NSRYGRIDEL KDLVGRLHDS GIKVLGDAVL 600 NHRCAHYQNK NGIWNIFGGR LSWDDRAVVA DDPHFQGRGN KSSGDNFHAA PNIDHSQDFV 660 RKDIKEWMGW LREEIGYDGW RLDFVRGFWG GYVKDYLDAT NPYFAVGEYW DSLSYTYGEM 720 DHNQDAHRQR IIDWINATNG SAGAFDVTSK GVLHAALEKC EYWRLADEKG KPPGVVGWWP 780 SRAVTFVENH DTGSTQGHWR FPGGKEMQGY AYILTHPGTP AVFFDHIYSS NYRSEIAALI 840 AFRNRHKLHC RSVVKISKAV NDVYAAIIDD KVAMKIGPGR FEPPKGPRKW SLALEGRDYK 900 VWAA* 960 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| PRK09441 | PRK09441 | 5.0e-49 | 513 | 846 | 421 | + cytoplasmic alpha-amylase; Reviewed | ||
| PLN00196 | PLN00196 | 8.0e-136 | 513 | 902 | 403 | + alpha-amylase; Provisional | ||
| cd11314 | AmyAc_arch_bac_plant_AmyA | 2.0e-161 | 514 | 854 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
| PLN02361 | PLN02361 | 1.0e-169 | 510 | 902 | 398 | + alpha-amylase | ||
| PLN02784 | PLN02784 | 0 | 79 | 902 | 834 | + alpha-amylase | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004556 | alpha-amylase activity |
| GO:0005509 | calcium ion binding |
| GO:0005975 | carbohydrate metabolic process |
| GO:0043169 | cation binding |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAX33231.1 | 0 | 2 | 904 | 1 | 900 | plastid alpha-amylase [Malus x domestica] |
| EMBL | CAN69906.1 | 0 | 66 | 902 | 51 | 884 | hypothetical protein [Vitis vinifera] |
| EMBL | CBI32016.1 | 0 | 66 | 902 | 51 | 882 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002270049.1 | 0 | 66 | 902 | 51 | 898 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002520134.1 | 0 | 2 | 904 | 1 | 899 | alpha-amylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2qpu_C | 0 | 512 | 902 | 1 | 402 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_B | 0 | 512 | 902 | 1 | 402 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 2qpu_A | 0 | 512 | 902 | 1 | 402 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
| PDB | 3bsg_A | 0 | 512 | 902 | 1 | 402 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
| PDB | 2qps_A | 0 | 512 | 902 | 1 | 402 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EG631183 | 830 | 89 | 904 | 0 |
| HO826981 | 403 | 501 | 902 | 0 |
| ES805448 | 328 | 470 | 797 | 0 |
| DR932783 | 288 | 513 | 800 | 0 |
| HO826981 | 20 | 468 | 487 | 0.11 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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